Detection method applied to membrane separation and membrane transparent liquid

A detection method and technology for transparent liquids, which are applied in semi-permeable membrane separation, chemical instruments and methods, membranes, etc., and can solve problems such as inability to count, inability to carry out, and difficulty.

Active Publication Date: 2012-07-18
HUNAN TECH NEW MEDICAL SYST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In many experiments, it is necessary to observe and count the concentrated substances, but in the existing detection experiments, due to the non-transparent or translucent filter membrane, it is necessary to directly observe and count the substances concentrated on the filter membrane. difficult or even impossible
For example, in the process of tuberculosis testing, membrane separation can be used to concentrate Mycobacterium tuberculosis, and the Mycobacterium tuberculosis concentrated by membrane separation adheres to the filter membrane. It becomes difficult to observe and count Mycobacterium tuberculosis; another example is the air quality detection experiment, which ge

Method used

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  • Detection method applied to membrane separation and membrane transparent liquid

Examples

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Embodiment 1

[0029] Embodiment one: the present invention is used for tubercle bacillus detection experiment:

[0030] The experimental process includes: making the sample to be tested→→using the membrane separation device to concentrate the sample, and the Mycobacterium tuberculosis in the sample to be tested adheres to the filter membrane→→staining and cleaning the bacteria on the filter membrane→→adding to the membrane separation device Alcohol, remove other liquids in the filter membrane pores, at this time, there is only alcohol in the filter membrane pores → → volatilize the alcohol in the filter membrane pores, the filter membrane pores are filled with air, and the filter membrane is opaque at this time → → Take out the filter membrane from the membrane separation device, add the membrane transparent liquid of the present invention on the filter membrane, fill the membrane transparent liquid in the filter membrane pores, and make the filter membrane transparent → → observe and count....

Embodiment 2

[0032] Embodiment two: the present invention is used for cell detection experiment:

[0033] The experimental process includes: making the sample to be tested→→using the membrane separation device to concentrate the sample, and the cells in the sample to be tested are attached to the filter membrane→→staining and cleaning the cells on the filter membrane→→take out the filter membrane from the membrane separation device , add the membrane transparent liquid of the present invention on the filter membrane, the filter membrane hole is filled with the membrane transparent liquid, the filter membrane is transparent→→observe and analyze.

[0034] The composition of the film transparent liquid of the present invention in the above examples is: mixed with water and glycerin, and the volume ratio between water and glycerin is 1:3.0.

Embodiment 3

[0035] Embodiment three: for air quality detection experiment:

[0036] The experimental process includes: air sampling → → use the membrane separation device to concentrate the sample, the suspended matter in the air sample adheres to the filter membrane, and the filter membrane is opaque at this time → → take out the filter membrane from the membrane separation device, pour it into the filter membrane Add membrane transparent liquid of the present invention, filter membrane hole is filled with membrane transparent liquid, filter membrane is transparent→→observe and count.

[0037] The film transparent liquid of the present invention in the above-mentioned embodiment is composed of: water and glycerin are mixed, and the volume ratio between water and glycerin is 1: 3.0; The composition of the film transparent liquid of the present invention can also be: paraffin oil and methyl alcohol Silicone oil is mixed, and the volume ratio between paraffin oil and methyl silicone oil is ...

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Abstract

The invention discloses a detection method which is used for making a filtering membrane transparent in the process of performing inspection and detection by using a membrane separation technology in fields of medical science, biology, environtology and the like and is applied to membrane separation and membrane transparent liquid. According to the detection method applied to the membrane separation, the membrane transparent liquid is added into the filtering membrane in the process of detecting ingredients entrapped by the filtering membrane, so that the filtering membrane is in the transparent state; the filtering membrane has the performance of a transparent material, and the prepared filtering membrane is in the nontransparent or semitransparent state; and an error of the refractive index of the membrane transparent liquid and the refractive index of the material of the filtering membrane is within +/-10 percent. By the membrane transparent liquid, the filtering membrane becomes transparent, so that substances which are adhered to the filtering membrane can be observed and counted directly.

Description

technical field [0001] The invention relates to a membrane separation detection method and a membrane transparent liquid for making a filter membrane transparent in the fields of medicine, biology, environmental science and the like during the inspection and detection process. Background technique [0002] Membrane separation refers to the method of separating the components in the liquid material by using the pressure difference as the driving force, using the membrane as the separation medium, and relying on the selectivity of the membrane; including filtration membranes, ultrafiltration membranes, etc. For example, if the filter membrane is used as the separation medium, by applying a certain pressure difference on both sides of the filter membrane, the substances whose volume is smaller than the pore size of the filter membrane in the component of the raw liquid will pass through, while the substances in the component of the raw solution whose volume is larger than the po...

Claims

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Application Information

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IPC IPC(8): B01D65/10
CPCB01D65/10B01D2325/44B01D61/10
Inventor 彭钧
Owner HUNAN TECH NEW MEDICAL SYST
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