Probiotic drops for improving infantile diarrhea
A technology of infant diarrhea and probiotics, which is applied in the field of medicine and biology, can solve the problems of low efficiency of emulsification machine, poor emulsification effect, insufficient stirring and mixing, etc., and achieve the effect of enhancing emulsification effect, easy cleaning, and easy stirring
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Embodiment 1
[0083] S1:
[0084] (1) Select animal Bifidobacterium strains and dilute them with warm water at 25-40°C;
[0085] (2) In a sterile environment, take out the strain with an inoculation needle and put it into a conical flask equipped with a culture medium, seal the mouth of the bottle and put it into an incubator, adjust the temperature of the incubator to 35°C, and let it stand for 12 hours;
[0086] (3) Carry out empty sterilization of the fermenter, then adjust the sterilization temperature to 125°C, adjust the pressure inside the tank to 0.05MPa, put it into the medium for sterilization, and keep it for 15min, finally cool down to 35°C, and reduce the pressure to 0.01MPa;
[0087] (4) Inoculate probiotics, pass sterile air into the fermenter, place the inoculation flame circle at the inoculation port, open the inoculation cover after igniting, and place the triangular flask above the flame circle, open the triangular flask, and put the bacteria into it In the tank, tighte...
Embodiment 2
[0102] S1:
[0103] (1) Select animal Bifidobacterium strains and dilute them with warm water at 25-40°C;
[0104] (2) In a sterile environment, use an inoculation needle to take out the strain and put it into a conical flask equipped with a medium, seal the mouth of the bottle and put it into an incubator, adjust the temperature of the incubator to 38°C, and let it stand for 24 hours;
[0105] (3) Carry out empty sterilization of the fermenter, then adjust the sterilization temperature to 130°C, adjust the pressure inside the tank to 0.10MPa, put it into the medium for sterilization, and keep it for 30min, finally cool down to 38°C, and reduce the pressure to 0.01MPa;
[0106] (4) Inoculate probiotics, pass sterile air into the fermenter, place the inoculation flame circle at the inoculation port, open the inoculation cover after igniting, and place the triangular flask above the flame circle, open the triangular flask, and put the bacteria into it In the tank, tighten the ...
Embodiment 3
[0121] S1:
[0122] (1) Select animal Bifidobacterium strains and dilute them with warm water at 25-40°C;
[0123] (2) In a sterile environment, use an inoculation needle to take out the strain and put it into a conical flask equipped with a medium, seal the mouth of the bottle and put it into an incubator, adjust the temperature of the incubator to 40°C, and let it stand for 48 hours;
[0124] (3) Carry out empty sterilization of the fermenter, then adjust the sterilization temperature to 135°C, adjust the pressure in the tank to 0.15MPa, put it into the medium for sterilization, and keep it for 45min, finally cool down to 40°C, and reduce the pressure to 0.01MPa;
[0125] (4) Inoculate probiotics, pass sterile air into the fermenter, place the inoculation flame circle at the inoculation port, open the inoculation cover after igniting, and place the triangular flask above the flame circle, open the triangular flask, and put the bacteria into it In the tank, tighten the inoc...
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