37 results about "Ammoniacum" patented technology

The invention discloses a method for detecting quality of alkaloids in a Tibetan medicine herba aconiti tangutici and preparations thereof. The method comprises the steps of identification, check and content determination, wherein thin layer chromatography is adopted for identification and is characterized by taking a herba aconiti tangutici control medicine as the control and normal hexane-ethyl acetate-methanol-ammonia water as a developer; high performance liquid chromatography is adopted for check and is characterized by taking a mixed solution of an aconitine control substance, a hypaconitine control substance and a new aconitine control substance as the control, acetonitrile-tetrahydrofuran as a mobile phase A and an ammonium acetate water solution with glacial acetic acid as a mobile phase B to carry out gradient elution; high performance liquid chromatography is adopted for hordenine content determination and is characterized by taking a hordenine control substance as the control and an acetonitrile-potassium dihydrogen phosphate water solution as a mobile phase; and back titration is adopted for total alkaloid content determination. The method is stable and reliable, has strong specificity, can ensure the safety, effectiveness and quality controllability of the alkaloids in the Tibetan medicine herba aconiti tangutici and the preparations thereof, and has higher scientificity and application values.

The invention discloses an alkaloid extract of trembling poplar as well as an extraction method and application thereof. The extraction method of the alkaloid extract of trembling poplar comprises the following steps: baking a trembling poplar plant material at 35 DEG C, crushing to obtain fine powder with the granularity of about 40 meshes; infiltrating with 10% ammonia water for 12 hours, and filtering to obtain residues; and 1 hour later, refluxing by use of 2L of chloroform for 2 hours, filtering, concentrating, extracting five times, 100mL per time, by use of 1-2% hydrochloric acid, regulating the pH value of the hydrochloric acid extract to be more than 8 by use of 10% ammonia water, extracting with chloroform for 3 times, 100mL per time, filtering, combining, and evaporating to dryness, thus obtaining the alkaloid extract. The extract method is simple and convenient and the yield of alkaloid is high; the alkaloid extract of the trembling poplar has excellent capability of inhibiting growth, proliferation and transfer of tumor cells, has wide application in preparing a medicament for treating cancer cells, has certain practicality, and can generate good economical and social benefits.

The invention provides a method for extracting polysaccharides from morinda root and the application of the polysaccharides in cigarettes. The method for extracting the polysaccharides comprises the steps as follows: morinda root is dried, and pulverized into powder of 10 to 100 meshes; the morinda root powder is immersed and extracted by adding water at 50 to 100 DEG C; subsequently, the mixtureof the morinda root powder and water is filtered and the filtrate is concentrated; the concentrated filtrate is added to ethanol to ensure the ethanol to account for 80 percent in volume; the mixtureof the concentrated filtrate and the ethanol are allowed to be settled out and filtered, thus getting the raw polysaccharides; the raw polysaccharides are put in water for redissolution; a sevag reagent 0.1 to 1 time of the raw polysaccharide solution in volume is added, and is shaken and allowed to be separated into layers; the mixture of the thick sevag and protein on the lower layer is removed; the pH value is adjusted to 9 to 10 with ammonia; oxydol 30 percent of the solution in mass is added slowly until the solution is turned into straw yellow; after the solution is decolored, the solution is condensed before ethanol with the concentration from 95 to 100 percent is added, the volume percentage of the ethanol is kept to be 80 percent; the solution is allowed to be settled out for 24 to 48 hours, thus getting the polysaccharides from morinda root through filtration.

The invention relates to a method for rejuvenating and culturing seedlings of sianchau, which comprises the following steps: 1, culturing a mother plant of the sianchau, namely culturing a sinachau plant which is robust without insect disease in a vigorous growth period of the sianchau; and 2, culturing the seedlings by cutting, namely from February to April of the next year, selecting good mother plants, shearing cutting wood, performing slurry dipping treatment on base parts of the cutting wood, arranging the cutting wood in a dug channel on a sianchau culturing field, covering soil to makethe length of the cutting wood exposed out of the ground half to one fourth of the total length, watering rooting water thoroughly, covering a thin film and a sunshade net to keep the soil moist, after 9 to 12 days, removing the thin film and the sunshade net, thinly watering 0.1 to 0.5 percent aqueous solution of ammonium carbonate to strengthen the seedlings, and after 15 to 25 days, digging the seedlings with the soil and transplanting the seedlings to a planting field. The method ensures that the seedlings of the sianchau have high survival rate, good quality, consistent growth in the field at a later stage, low disease rate, high yield and convenient to manage, and can greatly reduce cost, labor force investment and consumption of agricultural chemicals, and improve product quality.