Paper chip, making method thereof, and bio-molecule detection method
A detection method and paper chip technology, which is applied in the field of paper chips, can solve problems such as unsuitability for clinical medical analysis, limitation of quantum dot application, quantum dot toxicity, etc., and achieve the effects of direct detection, reduced detection cost, and high accuracy
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[0065] The present invention also provides a method for preparing a paper chip, comprising:
[0066] forming a detection zone on a paper-based material;
[0067] adding a fluorescent donor material solution to the detection area, the fluorescent donor material includes up-conversion fluorescent nanomaterials and surface markers marked on the up-conversion fluorescent nanomaterials;
[0068] removing the solvent in the fluorescent donor material solution to obtain a paper-based material immobilized with the fluorescent donor material;
[0069] The paper-based material with the fluorescent donor material immobilized and the fluorescent quencher form a paper chip.
[0070] The present invention uses a paper-based material as the basic structure of the paper core, and the paper-based material is also called a paper-based carrier, which can be whatman No. 1 filter paper, office printing paper, etc., preferably office printing paper. For paper-based materials, since the additives ...
Embodiment 1
[0118] Take 2 mL of 0.25mol / L rare earth nitrate solution, wherein the rare earth ions are yttrium ions, ytterbium ions and erbium ions with a molar ratio of 80:18:2, add 18 mL of absolute ethanol, and then add 0.9000 g of polyacrylic acid ( 8 mL of an aqueous solution with a molar ratio of polyacrylic acid and rare earth ions of 1:1), stirred for 10 min; then added 0.2100 g of sodium fluoride (F - / Rare earth ions molar ratio of 10:1) aqueous solution 8mL, after stirring for 20min, placed in a high-pressure reactor, and reacted at 200 ℃ for 10h under stirring conditions; stop heating and keep stirring to cool to room temperature, centrifuge to separate the solid product, Washed with absolute ethanol and ultrapure water for 3 times each, and vacuum-dried for 12 h at room temperature to obtain polyacrylic acid-modified upconversion fluorescent nanomaterials;
[0119] Dissolve 5 mg of polyacrylic acid-modified up-conversion fluorescent nanomaterials in 5 mL of 10 mM MES buffer ...
Embodiment 2
[0123] Take 2 mL of 0.25mol / L rare earth nitrate solution, wherein the rare earth ions are yttrium ions, ytterbium ions and erbium ions with a molar ratio of 80:18:2, add 18 mL of absolute ethanol to it, and then add 0.3400 g of polyethylene oxide. Aqueous solution of amine 8mL, stir for 10min; then add 0.1260g sodium fluoride (F - / Rare earth ions molar ratio of 6:1) aqueous solution 8mL, after stirring for 20min, placed in the autoclave, under stirring conditions at 200 ℃ for 4h reaction; stop heating and keep stirring to cool to room temperature, centrifuge to separate the solid product, Washing with absolute ethanol and ultrapure water for 3 times, vacuum drying at room temperature for 12 h to obtain polyethyleneimine-modified upconversion fluorescent nanomaterials;
[0124] Dissolve 5 mg of polyethyleneimine-modified upconversion fluorescent nanomaterials in 5 mL of 10 mM MES buffer with a pH of 5.5, add 3.2 mg of EDC·HCl and 6.6 mg of Sulfo-NHS, and incubate at 30 °C fo...
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