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Paper chip, making method thereof, and bio-molecule detection method

A detection method and paper chip technology, which is applied in the field of paper chips, can solve problems such as unsuitability for clinical medical analysis, limitation of quantum dot application, quantum dot toxicity, etc., and achieve the effects of direct detection, reduced detection cost, and high accuracy

Active Publication Date: 2015-07-29
GUANGZHOU IMPROVE MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] (1) The detection of biological macromolecules on a paper chip is usually performed by enzyme-linked immunosorbent reaction, which requires washing and separation, which is cumbersome and time-consuming. High testing cost;
[0006] (2) The additives in the paper substrate will produce a rather high background signal. The current fluorescence detection method on the paper chip cannot avoid this defect, which limits the improvement of its sensitivity;
[0007] (3) Quantum dots have certain toxicity and are not suitable for clinical medical analysis, which limits the application of quantum dots in the development of paper chip methods

Method used

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  • Paper chip, making method thereof, and bio-molecule detection method
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  • Paper chip, making method thereof, and bio-molecule detection method

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preparation example Construction

[0065] The present invention also provides a method for preparing a paper chip, comprising:

[0066] forming a detection zone on a paper-based material;

[0067] adding a fluorescent donor material solution to the detection area, the fluorescent donor material includes up-conversion fluorescent nanomaterials and surface markers marked on the up-conversion fluorescent nanomaterials;

[0068] removing the solvent in the fluorescent donor material solution to obtain a paper-based material immobilized with the fluorescent donor material;

[0069] The paper-based material with the fluorescent donor material immobilized and the fluorescent quencher form a paper chip.

[0070] The present invention uses a paper-based material as the basic structure of the paper core, and the paper-based material is also called a paper-based carrier, which can be whatman No. 1 filter paper, office printing paper, etc., preferably office printing paper. For paper-based materials, since the additives ...

Embodiment 1

[0118] Take 2 mL of 0.25mol / L rare earth nitrate solution, wherein the rare earth ions are yttrium ions, ytterbium ions and erbium ions with a molar ratio of 80:18:2, add 18 mL of absolute ethanol, and then add 0.9000 g of polyacrylic acid ( 8 mL of an aqueous solution with a molar ratio of polyacrylic acid and rare earth ions of 1:1), stirred for 10 min; then added 0.2100 g of sodium fluoride (F - / Rare earth ions molar ratio of 10:1) aqueous solution 8mL, after stirring for 20min, placed in a high-pressure reactor, and reacted at 200 ℃ for 10h under stirring conditions; stop heating and keep stirring to cool to room temperature, centrifuge to separate the solid product, Washed with absolute ethanol and ultrapure water for 3 times each, and vacuum-dried for 12 h at room temperature to obtain polyacrylic acid-modified upconversion fluorescent nanomaterials;

[0119] Dissolve 5 mg of polyacrylic acid-modified up-conversion fluorescent nanomaterials in 5 mL of 10 mM MES buffer ...

Embodiment 2

[0123] Take 2 mL of 0.25mol / L rare earth nitrate solution, wherein the rare earth ions are yttrium ions, ytterbium ions and erbium ions with a molar ratio of 80:18:2, add 18 mL of absolute ethanol to it, and then add 0.3400 g of polyethylene oxide. Aqueous solution of amine 8mL, stir for 10min; then add 0.1260g sodium fluoride (F - / Rare earth ions molar ratio of 6:1) aqueous solution 8mL, after stirring for 20min, placed in the autoclave, under stirring conditions at 200 ℃ for 4h reaction; stop heating and keep stirring to cool to room temperature, centrifuge to separate the solid product, Washing with absolute ethanol and ultrapure water for 3 times, vacuum drying at room temperature for 12 h to obtain polyethyleneimine-modified upconversion fluorescent nanomaterials;

[0124] Dissolve 5 mg of polyethyleneimine-modified upconversion fluorescent nanomaterials in 5 mL of 10 mM MES buffer with a pH of 5.5, add 3.2 mg of EDC·HCl and 6.6 mg of Sulfo-NHS, and incubate at 30 °C fo...

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Abstract

A paper chip, a preparation method thereof and a detection method for biological molecules are provided. The paper chip includes a paper-based material, a fluorescent donor and a fluorescent quenching agent. A detection zone is arranged on the paper-based material, and the fluorescent donor material is secured within the detection zone of the paper-based material. The fluorescent donor material includes an up-conversion luminescence nano-material and a surface marker marked on the up-conversion luminescence nano-material. The fluorescence quenching agent includes an organic dye or a fluorescent receptor. The combination of the up-conversion luminescence analysis and the paper chip technology decreases the amount of reactants used, reduces testing costs, speeds up the reaction rate of the reactants, and increases the possibility of applying the paper chips to the clinical testing.

Description

technical field [0001] The invention belongs to the technical field of paper chips, and in particular relates to a paper chip, a preparation method thereof and a detection method of biomolecules. Background technique [0002] The paper chip is a device proposed by the whitesides research group in 2007 to meet the development requirements of the POCT diagnostic platform. It has the potential to develop into a new type of inexpensive testing platform, providing medical diagnosis and health monitoring that were not available in developing countries and remote areas, and playing a huge role in promoting the popularization of personalized medicine. [0003] There are many methods for making paper chips. Common methods mainly include photolithography, paraffin printing, lithography, drawing, inkjet printing, plasma etching and cutting. So far, the method of making paper chips has become more and more mature, and the research focus has shifted to the use of paper chips for biologi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N21/64
CPCG01N21/6428G01N2021/6432G01N33/521G01N33/542
Inventor 刘志洪何梦媛吴正俊杨利
Owner GUANGZHOU IMPROVE MEDICAL TECH CO LTD
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