Medical application of berberine derivative
A berberine and drug technology, which is applied in the field of berberine derivatives, can solve the problems of unseen glioma migration and invasion, and unsatisfactory drug efficacy.
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[0030] Synthesis and Identification of Berberine Derivatives
[0031]Berberine hydrochloride was purchased from Xi’an Xiaocao Plant Technology Co., Ltd., with a purity of 97%; bromododecane, bromohexadecane, bromooctadecane, and benzyl bromide were all analytically pure, purchased from Aladdin Reagent Co., Ltd. Company; N, N-dimethylformamide (DMF) is analytically pure, dried with molecular sieves, and purchased from Tianjin Fuyu Fine Chemical Co., Ltd.; neutral alumina for chromatography is a product of Sinopharm Group; DMEM medium (R10 -013-cv), fetal bovine serum (FBS), trypsin, penicillin and streptomycin were all purchased from Cellgro, USA; tetramethylazozolium salt (MTT) and dimethyl sulfoxide (DMSO) were purchased from Sigma Company of the United States; 4% paraformaldehyde was purchased from Guangzhou Jingxin Technology Co., Ltd.; the experimental water was ultrapure water. Rat glioma C6 cells and human glioma U87 cells were from the ATCC cell bank in the United St...
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