Stimuli-responsive astaxanthin nanoparticles, preparation method thereof and application of stimuli-responsive astaxanthin nanoparticles to directions of mitochondrial targeting and colitis relieving

A nanoparticle and stimuli-responsive technology, which is applied in applications, anti-inflammatory agents, microcapsules, etc., can solve the problems of lack of stimuli-responsive loading capacity and difficulty in targeted and concentrated release of nutritional functional factors, so as to improve absorption Utilization, enhanced targeting, effect of alleviating colonic inflammation

Pending Publication Date: 2021-04-02
DALIAN POLYTECHNIC UNIVERSITY
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned traditional delivery system does not have a stimulus-responsive delivery capacity, and it is also difficult to achieve ta

Method used

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  • Stimuli-responsive astaxanthin nanoparticles, preparation method thereof and application of stimuli-responsive astaxanthin nanoparticles to directions of mitochondrial targeting and colitis relieving
  • Stimuli-responsive astaxanthin nanoparticles, preparation method thereof and application of stimuli-responsive astaxanthin nanoparticles to directions of mitochondrial targeting and colitis relieving
  • Stimuli-responsive astaxanthin nanoparticles, preparation method thereof and application of stimuli-responsive astaxanthin nanoparticles to directions of mitochondrial targeting and colitis relieving

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preparation example Construction

[0034] A method for preparing astaxanthin nanoparticles with pH response, mitochondrial targeting and inhibition, and relief of colonic inflammation, comprising the following steps:

[0035] S1. Preparation of TPP carboxyl activation solution: Weigh (3-carboxypropyl) triphenylphosphine bromide (TPP) and dissolve it in 2-(N-morpholine) ethanesulfonic acid (MES) buffer solution, so that the final TPP The concentration is 4~8mg / mL, magnetically stirred at 600~800rpm, fully dissolved; then add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxy Succinimide, magnetic stirring 600-800rpm, reaction time 3h-5h;

[0036] S2. Preparation of chitosan-TPP complex: take chitosan and fully dissolve it in glacial acetic acid solution, so that the final concentration of chitosan is 4-8 mg / mL; gradually add chitosan solution to the In the TPP carboxyl activation solution, the magnetic stirring is 600-800rpm, and the reaction time is 6-10h; after the reaction, the chitosa...

Embodiment 1

[0042] An astaxanthin nanoparticle with pH response, mitochondrial targeting, and colonic inflammation relief, comprising the following components: casein content 66.22% w / w, chitosan-TPP complex content 8.27% w / w , the content of sodium alginate is 24.83% w / w, and the content of astaxanthin is 0.66% w / w.

[0043] The preparation method of the astaxanthin nanoparticles with pH response, mitochondrial targeting and relief of colonic inflammation comprises the following steps:

[0044] S1. Preparation of TPP carboxyl activation solution: Weigh (3-carboxypropyl)triphenylphosphine bromide (TPP) and dissolve it in 0.1M pH 5.0 2-(N-morpholine)ethanesulfonic acid (MES) buffer solution , so that the final concentration of TPP is 8mg / mL, fully stirred and dissolved under the condition of 600rpm; then add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride to the final concentration of TPP solution The final concentration of N-hydroxysuccinimide is 8mg / mL and 4mg / mL, and the re...

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Abstract

The invention discloses astaxanthin nanoparticles as well as a preparation method and application thereof. The astaxanthin nanoparticles comprise 58%-68% w/w of casein, 7%-11% w/w of a chitosan-TPP compound, 24%-28% w/w of sodium alginate and 0.5%-7% w/w of astaxanthin. According to the astaxanthin nanoparticles, casein I is used for primary embedding of the astaxanthin, the chitosan-TPP compoundand the sodium alginate are further subjected to layer-by-layer self-assembly through electrostatic interaction, and pH response type and mitochondrial targeting type nanoparticles are constructed andformed. According to the method, gastric acid escape can be achieved, the release rate of the astaxanthin in intestinal tract is increased, compared with the mode that free astaxanthin can be concentrated and enriched in the colons of mice, the colitis of the mice is relieved, and the targeting effect on cell mitochondria is achieved, the embedding protection mode of the method constructs a functional characteristic nanometer carrying system, and the absorption and utilization degree of nutrients is fully improved.

Description

technical field [0001] The invention relates to the technical field of food and health food, in particular to an astaxanthin nanoparticle with pH response, mitochondrial targeting and colonic inflammation relief and a preparation method thereof. Background technique [0002] Astaxanthin (3,3'-dihydroxy-4,4'-diketo-β,β'-carotene) is a ketone carotenoid, the appearance of astaxanthin is a red solid powder, it has lipid Soluble, insoluble in water, astaxanthin mostly exists in the form of monoester and diester, the free form of astaxanthin is relatively low, mostly exists in microalgae and aquatic animals, and has color development. It has a variety of biological activities such as anti-oxidation, anti-inflammation, anti-lipid peroxidation, anti-aging and nerve protection, etc., especially anti-oxidation is the most prominent biological activity of astaxanthin, which is mainly due to the variety of astaxanthin Saturated conjugated double bond structure, and there are ketone gr...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K47/36A61K47/42A61K31/122A61P1/00A61P29/00A23L33/00
CPCA61K9/5161A61K9/5169A61K31/122A61P1/00A61P29/00A23L33/00A23V2002/00A23V2200/30A23V2250/54246A23V2250/5026A23V2250/2104A23V2300/20A23V2300/10A23V2200/32
Inventor 谭明乾张雪迪赵雪铁珊珊李佳璇吕玥琪袁龙
Owner DALIAN POLYTECHNIC UNIVERSITY
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