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Self-oxygen-supply hollow Prussian blue nanoparticle as well as preparation method and application thereof

A technology of Prussian blue and self-supplying oxygen, which is applied in the fields of medical formula, photodynamic therapy, medical preparations containing active ingredients, etc., can solve problems such as human side effects, weak anti-oxidation ability of hemoglobin, and reduced anti-tumor effect of PDT, etc., to achieve Effects of promoting apoptosis, improving oxygen supply deficiency, and increasing drug loading

Active Publication Date: 2021-10-01
HUAQIAO UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]The hypoxic microenvironment of the tumor leads to a markedly reduced tumor suppressive effect of PDT, especially in cases requiring continued therapy
Hemoglobin can combine with oxygen to supply oxygen to various tissues and organs, but free hemoglobin has weak antioxidant capacity and has side effects on the human body, so it cannot be directly used as an oxygen carrier for cancer treatment

Method used

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  • Self-oxygen-supply hollow Prussian blue nanoparticle as well as preparation method and application thereof
  • Self-oxygen-supply hollow Prussian blue nanoparticle as well as preparation method and application thereof
  • Self-oxygen-supply hollow Prussian blue nanoparticle as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Preparation of Prussian Blue Nanoparticles

[0036] Weigh 528 mg of potassium ferricyanide and 12.0 g of polyvinylpyrrolidone in a beaker, add 160 mL of hydrochloric acid with a concentration of 0.01 M, stir magnetically for 0.5 h, transfer the reaction solution to a 200 mL reaction kettle, and place it in an oven at 80 °C for constant temperature reaction for 20 h After centrifugation, the obtained precipitate was washed three times with ultrapure water, and Prussian blue nanoparticles were obtained after freeze-drying (such as figure 1 shown).

Embodiment 2

[0037] Example 2: Preparation of hollow Prussian blue nanoparticles

[0038] 20 mg of Prussian blue nanoparticles obtained in Example 1 were weighed and dispersed in 20 mL of hydrochloric acid solution with a concentration of 1 M, and 100 mg of polyvinylpyrrolidone was added. Then the reaction system was placed in an oven and reacted at 140° C. for 4 h. After the reaction, the hollow Prussian blue nanoparticles were collected by centrifugation, washed 3 times with deionized water, and then freeze-dried to obtain the hollow Prussian blue nanoparticles (such as figure 1 shown).

[0039] According to the X-ray diffraction patterns of the products of Example 1 and Example 2 ( figure 2 ), the positions of all diffraction peaks correspond to the diffraction surfaces of Prussian blue nanoparticles, respectively, showing the formation of Prussian blue nanoparticles; TEM images of Prussian blue nanoparticles ( image 3 ), it can be seen that the Prussian blue nanoparticles have a u...

Embodiment 3

[0040] Example 3: Loading of Drugs

[0041]Take 5 mg of the lyophilized powder of hollow Prussian blue nanoparticles prepared in Example 2 in a flask, add ultrapure water to disperse. Weigh an appropriate amount of hemoglobin into a centrifuge tube, add ultrapure water to dissolve to a concentration of 1 mg / mL, drop 5 mL or 10 mL of Hb solution into the flask dropwise, and stir for 12 h in the dark under nitrogen atmosphere. After the reaction, centrifuge, add ultrapure water and wash 2 times to obtain self-oxygenated hollow Prussian blue nanoparticles loaded with hemoglobin (such as figure 1 and Figure 5 shown). Weigh 5 mg of IR783 into a brown glass bottle, add an appropriate amount of ultrapure water to dissolve, and prepare a solution of IR783 with a concentration of about 200 μg / mL. Then weigh 5 mg of hemoglobin-loaded self-oxygenated hollow Prussian blue nanoparticles into a beaker, and add ultrapure water to disperse. Subsequently, the IR783 solution was added drop...

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Abstract

The invention discloses a self-oxygen-supply hollow Prussian blue nanoparticle and a preparation method and application thereof. The self-oxygen-supply hollow Prussian blue nanoparticle is provided with a hollow Prussian blue nanoparticle body, hemoglobin and IR783 are loaded on a mesoporous shell layer and an internal hollow structure of the hollow Prussian blue nanoparticle body, the drug loading capacity of the hemoglobin is 61.5%-63.5%, and the drug loading capacity of the IR783 is 24.2%-27.5%. The self-oxygen-supply hollow Prussian blue nanoparticle loaded with hemoglobin and IR783 prepared by the invention can improve the condition of insufficient oxygen supply in photodynamic therapy; in addition, since the half-life period of active oxygen generated by the photodynamic therapy is very short and the active oxygen is effective only in a relatively short distance, loading of IR783 with a mitochondrial targeting function is conducted in the invention, the self-oxygen-supply hollow Prussian blue nanoparticle body loaded with hemoglobin and IR783 is conveyed to mitochondria, so a mitochondrial function is destroyed, photodynamic therapy effect is remarkably improved, and tumor cell apoptosis is promoted.

Description

technical field [0001] The invention belongs to the technical field of drug carriers, in particular to a self-oxygenated hollow Prussian blue nanoparticle and a preparation method and application thereof. Background technique [0002] Malignant tumors seriously threaten human life and health. It is reported that in 2020, there will be 19.3 million new cancer cases and 10 million deaths. Traditional cancer treatment methods such as surgery, radiotherapy and chemotherapy have been verified by many cases and are highly reliable. However, malignant tumors are easy to metastasize and have strong drug resistance, and their anti-tumor effects are not satisfactory. Photodynamic and photothermal therapy have attracted the attention of researchers as a class of non-invasive cancer treatment methods. They have the characteristics of local therapy and can treat tumor tissues, killing tumor cells and reducing damage to normal tissues. . Among them, photodynamic therapy uses a laser to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K33/26A61K9/51A61K47/02A61K47/42A61P35/00
CPCA61K41/0057A61K41/0052A61K33/26A61K9/5115A61K9/5169A61K9/5192A61P35/00A61K2300/00
Inventor 刘源岗王士斌龙瑞敏朱明智
Owner HUAQIAO UNIVERSITY
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