Viscosity fluorescent probe with mitochondrial targeting function as well as preparation method and application thereof

Abstract

The invention discloses a viscosity fluorescent probe with a mitochondrial targeting function as well as a preparation method and application thereof. The structural formula of the fluorescent probe is shown in the specification. The preparation method comprises the following steps: dissolving 5-methyl-3-(2'-benzothiazolyl)-4-hydroxybenzaldehyde and 2-pyridylacetonitrile onium iodide into absoluteethyl alcohol, adding piperidine, heating and refluxing the mixture for 6 hours, cooling, filtering, precipitating, and recrystallizing the solid with ethanol to obtain a yellow solid, namely the probe (Z)-2-(2-(3-(2-benzothiazolyl)-4-hydroxy-5-methylphenyl)-1-cyanovinyl)-1-methylpyridine-1-iodide. The fluorescent probe has the advantages of excellent viscosity detection performance, excellent anti-interference capability and good mitochondria targeting positioning function, can be used for monitoring the viscosity change of mitochondria, cells, animal tissues and organs and mouse living bodies, and has a wide application prospect in the field of organism viscosity detection.

Description

technical field [0001] The invention relates to the field of functional materials, in particular to a viscous fluorescent probe with mitochondrial targeting function and its preparation method and application. Background technique [0002] Cellular viscosity is an important microenvironmental parameter that plays an important role in signaling as well as interactions between biomolecules. The abnormality of mitochondrial viscosity will cause the corresponding changes in physiological functions or the occurrence of diseases. Therefore, it is of great significance to accurately measure the changes of local microviscosity in the mitochondria of living cells. Using fluorescent probes to detect viscosity changes in living organisms is one of the current research hotspots. It can overcome the shortcomings of traditional viscosity measurement devices that can only be used for macroscopic fluids and cannot visually monitor the viscosity changes in the microenvironment of living orga...

AI Technical Summary

Problems solved by technology

Using fluorescent probes to detect viscosity changes in living organisms is one of the current research hotspots. It can overcome the shortcomings of traditional viscosity measurement devices that can only be used for macroscopic fluids and cannot visually monitor the viscosity changes in the microenvironment of living organisms. In recent years, it has been widely accepted. focus on

[0003] Although many fluorescent probes that respond to viscosity have been reported, most of the reported viscosity-responsive fluorescent probes have short emission wavelengths, cannot detect the viscosity of specific organelles, and still have fluorescence interference in low-viscosity media. Unable to realize all-round viscosity detection from subcellular organelle-cell-organ-living body and other shortcomings

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