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Anthropogenic lactobacilus fermentum grx07 and application thereof

A technology for Lactobacillus fermentum and fermented dairy products, which is applied in the application fields of human Lactobacillus fermentum, fermented dairy products and functional foods or medicines to achieve the effects of reducing blood lipids, enhancing expression, and reducing the percentage of apoptosis of hepatocytes

Active Publication Date: 2014-07-23
YANGZHOU YANGDA KANGYUAN DAIRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are relatively few research reports on human-sourced probiotics in alleviating alcoholic liver injury, and related functional foods are even rarer on the market.

Method used

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  • Anthropogenic lactobacilus fermentum grx07 and application thereof
  • Anthropogenic lactobacilus fermentum grx07 and application thereof
  • Anthropogenic lactobacilus fermentum grx07 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0172] Embodiment 1: the preparation of Lactobacillus fermentum grx07 starter

[0173] The original strain of Lactobacillus fermentum grx07 was inoculated in 12% skim milk (sterilized at 115°C for 20 minutes), cultured at 37°C for 18-24 hours until the curdled milk was activated for two generations, and used as a mother starter. Inoculate the mother starter into 12% skim milk (sterilized at 115°C for 20 minutes) at an inoculum amount of 3% to 5%, and incubate at 37°C for 18 to 24 hours until the curdled milk, at which time the number of viable bacteria can reach 10 9 ~10 10 cfu / mL to obtain Lactobacillus fermentum grx07 starter.

Embodiment 2

[0174] Example 2: Preparation of functional fermented milk using Lactobacillus fermentum grx07

[0175]Heat the pretreated milk to about 60°C, add 6% sucrose, fully dissolve, and homogenize under 20MPa pressure. After heat treatment at 95°C / 5min, cool to 38°C. Add the Lactobacillus fermentum grx07 starter prepared according to Example 1 according to the inoculum amount of 5%, ferment at 37°C for 14-18h to curd milk, and then refrigerate at 4°C after cooling to obtain Lactobacillus fermentum grx07 functional fermented milk.

Embodiment 3

[0176] Embodiment 3: Utilize Lactobacillus fermentum grx07 to prepare probiotic milk beverage

[0177] The skim milk powder is made into 350kg of 12% reconstituted skim milk, heat-treated at 95°C / 8-10min, and then cooled to 37-40°C. Inoculate Lactobacillus fermentum grx07 starter with 3%~5% inoculum amount, culture at 37°C for 20~24h, and control the terminal acidity at 155~170°T; add 650kg of sugar sterilized at 90~110°C / 5~10s, stabilize (the composition of the mixture is 0.9%~1.4% pectin and 13.8%~15.4% sucrose), after mixing evenly, it is homogeneous at 20~25MPa, then heat treatment at 72℃ / 15s, and cool to 15~ 20°C, adopt aseptic or hygienic filling to obtain 1000kg of Lactobacillus fermentum grx07 milk beverage.

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Abstract

The invention relates to anthropogenic lactobacilus fermentum grx07 with a protection role on chronic alcoholic liver damage and application thereof. The lactobacilus fermentum grx07 strain disclosed by the invention is a probiotic strain separated from intestinal tracts of people in a Rugao longevity village of Jiangsu, the strain is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.8874. The lactobacilus fermentum grx07 strain has significant in vitro antioxidant ability and pathogenic bacteria inhibition ability, the rat blood fat of chronic alcoholic liver damage and the toxin level in serum can be significantly reduced, expression of liver inflammatory cytokines is reduced, the inflammatory reaction of the liver is relieved, the expression quality of antioxidant specific protein Nrf2 of the liver is increased, and the liver cell apoptosis percentage is reduced. Therefore, the anthropogenic lactobacilus fermentum grx07 has a significant protective effect on the chronic alcoholic liver damage.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a strain of human Lactobacillus fermentum grx07 which has a protective effect on chronic alcoholic liver injury and the application of the strain in fermented milk products, functional foods or medicines. technical background [0002] Chronic liver disease caused by long-term heavy drinking is one of the important causes of human liver damage. In my country, with the improvement of people's living standards, the number of alcoholics has increased, and long-term alcoholism has also become the cause of liver damage after viral hepatitis. The second leading cause. Alcoholic liver injury usually manifests as fatty liver in the early stage, and then can develop into alcoholic hepatitis, alcoholic liver fibrosis and alcoholic liver cirrhosis. Therefore, it is of great significance to explore the pathogenesis and preventive interventions of alcoholic liver disease. [0003] The pathog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23C9/12A23L1/29A61K35/74A61P1/16C12R1/225A23L33/00A61K35/747
Inventor 顾瑞霞刘东黄玉军陈霞陈大卫郭飞翔赵堂彦蒋欣荣
Owner YANGZHOU YANGDA KANGYUAN DAIRY
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