Lactobacillus fermentum, culture of lactobacillus fermentum and preparation method of culture

A technology for fermenting lactobacillus and culture, applied in the field of microorganisms, can solve the problems of small audience, the application scope and use methods of probiotics are not expanded more widely, and the use methods are limited, so as to reduce the degree of damage and improve skin health. , the effect of inhibiting the invasion of pathogenic microorganisms

Active Publication Date: 2021-06-11
山东凤凰生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The target audience of the above technical solutions is relatively small, and the way of use is limited to oral administration. The scope of application and way of use of probiotics has not yet been broadened.

Method used

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  • Lactobacillus fermentum, culture of lactobacillus fermentum and preparation method of culture
  • Lactobacillus fermentum, culture of lactobacillus fermentum and preparation method of culture
  • Lactobacillus fermentum, culture of lactobacillus fermentum and preparation method of culture

Examples

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Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: Select freshly fermented Xinjiang yoghurt, use the MRS medium solid plate streaking method, culture in a 37°C incubator for 36 hours, pick a single colony for repeated purification and culture once, and observe the single colony morphology of Lactobacillus fermentum with the naked eye. Plates were prepared by Gram staining method for electron microscopy observation.

[0045] The results showed that the biological characteristics of Lactobacillus fermentum LF8005 (Lactobacillus fermentum LF8005) were as follows: the cell size of the strain was (0.5μm~0.7μm)×(0.8μm~3.0μm), the bacteria were rod-shaped, single or adult Right arrangement, Gram-positive; this strain can grow on MRS agar medium, the colony is milky white, round, with a moist surface, opaque, neat edges, and the bacteria are viscous to a certain extent when picked; the optimum growth temperature is 30 ~40℃, facultative anaerobic, grow at pH 4.5-9.0, the optimum pH is around 6.5.

Embodiment 2

[0046] Embodiment 2: Viscosity analysis of lactobacillus fermentum

[0047] Lactic acid bacteria from different sources were inoculated into MRS liquid medium, cultured at 37°C for 24 hours, and the viscosities of different fermentation broths were measured with a viscometer.

[0048] The test results are shown in Table 1:

[0049] Table 1 Viscosity analysis results of lactic acid bacteria fermentation broth from different sources

[0050] strain separate source place of origin Viscosity / cP LF8005 sour milk Xinjiang 24418 CK-1 sour milk Inner Mongolia 15740 CK-2 cheese Inner Mongolia 18636 CK-3 Tibetan Ling Mushroom Tibet 22015 CK-4 sour milk Tibet 18902 CK-5 Sauerkraut Heilongjiang 12841 CK-6 Pickle sichuan 15027

[0051] Comparing the fermentation liquid of lactic acid bacteria from different sources, the fermentation liquid of Lactobacillus fermentum LF8005 isolated from Xinjiang yogh...

Embodiment 3

[0052] Embodiment 3: Preparation of Lactobacillus fermentum LF8005 culture

[0053] A preparation method of Lactobacillus fermentum LF8005 culture, said preparation method comprising the steps of:

[0054] (1) Strain activation: transfer Lactobacillus fermentum LF8005 to MRS solid slant medium, and culture at 35-40°C for 12-16h;

[0055] (2) Primary seed preparation: take the cultivated slant strain, transfer it to MRS liquid medium under aseptic conditions, and culture it statically at 35-40°C for 12-20 hours to obtain the primary seed liquid;

[0056] (3) Secondary seed preparation: Take the primary seed liquid and transfer it to MRS liquid medium for expanded cultivation, the transfer amount is 2%-10%, and culture at 35-40°C for 12-20h to obtain the secondary seed liquid ;

[0057] (4) Fermentation tank culture: transfer the secondary seed liquid to the sterilized medium, the inoculum size is 5-10%, the culture temperature is 35-40°C, the static culture time is 10-24h, an...

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Abstract

The invention discloses a lactobacillus fermentum strain LF8005 which is preserved in China Center for Type Culture Collection on November 9, 2020, the address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M2020704. The invention also provides a lactobacillus fermentum culture and a preparation method thereof, and the lactobacillus fermentum culture has the effects of inhibiting escherichia coli, staphylococcus aureus and candida albicans, scavenging free radicals, resisting oxidation and preventing skin inflammation. The lactobacillus fermentum is separated from a traditional fermented dairy product, the safety of the lactobacillus fermentum is guaranteed, macromolecular active matter such as polysaccharide, glycoprotein and biotin can be secreted, so that the viscosity of fermentation liquor is increased, and the lactobacillus fermentum serves as a natural active component to be added and applied to skin care products. The product is low in irritation, high in safety and not prone to dependence, mainly improves the skin environment and performs prevention and protection on the skin, adapts to the development requirements of current skin care cosmetics and can be safely added and applied to skin care products.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, in particular to a lactobacillus fermentum, a culture medium of lactobacillus fermentum and a preparation method thereof. Background technique [0002] The surface of human skin is rich in microbial diversity, including a variety of beneficial bacteria, pathogenic bacteria and neutral bacteria. The skin micro-ecological balance formed by these microorganisms directly affects the health of the skin. Skin beneficial microbial flora and their metabolites can regulate skin function by inhibiting pathogenic microorganisms on the skin surface, maintaining skin moisture, and enhancing skin surface immunity, which is of great significance for establishing and maintaining skin health and homeostasis. [0003] Lactic acid bacteria are a type of beneficial bacteria that widely exist in animals and plants, fermented foods, and the environment, and play a decisive role in the balance, stability and hea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K8/99A61Q19/08A61Q17/00A61P17/00A61P31/02A61P31/04A61P31/10C12R1/225
CPCC12N1/20A61K8/99A61Q19/08A61Q17/005A61P17/00A61P31/02A61P31/04A61P31/10Y02A50/30
Inventor 王静单宝龙任宝涛张化朋张颜廷庄金丽刘虹
Owner 山东凤凰生物科技股份有限公司
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