Lactobacillus salivarius and freeze-dried preparation thereof and application of freeze-dried preparation

A technology of Lactobacillus salivarius and freeze-dried preparations, applied in the field of microorganisms, can solve problems such as threats to animal and human health, intestinal imbalance, etc.

Inactive Publication Date: 2012-09-12
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for a long time, the extensive use of antibiotics as feed additives not only inhibits and kills pathogenic bacteria, but also inhibits and kills the normal symbiotic flora in animals, making the intestinal tract unbalanced for a long time, which poses a threat to animal health and even human health. direct threat

Method used

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  • Lactobacillus salivarius and freeze-dried preparation thereof and application of freeze-dried preparation
  • Lactobacillus salivarius and freeze-dried preparation thereof and application of freeze-dried preparation
  • Lactobacillus salivarius and freeze-dried preparation thereof and application of freeze-dried preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1, the preparation of good lactobacillus of pig source

[0031] 1.1 Isolation, screening and identification of pig-derived lactic acid bacteria

[0032] Piglets were dissected, 1g of mucous membrane was scraped aseptically, placed in a triangular flask with glass beads filled with 9ml of sterile normal saline, fully shaken and shaken, and then 1.0ml of bacterial liquid was absorbed into 9.0ml of sterile normal saline In the test tube, this dilution is 10 -1 , repeat the above process, and make 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 of bacteria liquid. choose 10 -4 、10 -5 、10 -6 For three dilutions, absorb 0.1ml of the bacterial solution and drop it on the MRS medium plate, place it in an anaerobic tank at 37°C for 24-48 hours, pick colonies with different shapes and inoculate them on the MRS medium for pure culture , and then stored in a 4°C refrigerator for later use.

[0033] Then pick the pure cultures for staining, microscopic examination, and selec...

Embodiment 2

[0086] Embodiment 2, the influence of porcine-derived Lactobacillus salivarius preparation on performance indicators of nursery pigs and growing pigs

[0087] 2.1 Experimental animals and grouping

[0088] A total of 81 nursery pigs (Du×Chang×Da Sanyuan hybrid pigs) with similar age and body weight were selected and randomly divided into 3 groups, with 3 replicates in each group and 9 pigs in each replicate. Group 1 is the control group, which is fed with basal diet + antibiotics (add 100 mg of ampicillin arsenic acid per kilogram of feed), and test group 1 and test 2 add 0.2% and 0.4% Lactobacillus salivarius to the basal diet respectively Preparations (contain 7.00×10 per kilogram of complete feed 9 cfu and 1.40×10 10 cfu Lactobacillus salivarius), the test period was 60 days, the first 30 days were fed with the nursery diet, and the last 30 days were fed with the growth stage diet. The experiment was carried out in a closed pig house with a concrete floor. The pigs were ...

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Abstract

The invention discloses Lactobacillus salivarius and a freeze-dried preparation thereof and application of the freeze-dried preparation, and relates to an animal probiotic strain and a freeze-dried preparation thereof. The name of the Lactobacillus salivarius is Lactobacillus salivarius ZLS006; and the collection number of the Lactobacillus salivarius is CGMCC No. 5761. The invention also provides the freeze-dried preparation which is prepared from the Lactobacillus salivarius ZLS006. The test proves that the freeze-dried preparation can improve the conversion efficiency of feeds for swinery, increase the viable count of lactic acid bacteria in manure of the swinery, and reduce the content of Escherichia coli, and has the effect of improving the immunity of organisms.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Lactobacillus salivarius derived from porcine and its freeze-dried preparation and application. Background technique [0002] There are about 30 genera and more than 500 kinds of microorganisms in the intestinal tract of animals, which depend on each other and compete with each other to maintain the microecological balance of the intestinal tract. However, for a long time, the extensive use of antibiotics as feed additives not only inhibits and kills pathogenic bacteria, but also inhibits and kills the normal symbiotic flora in animals, making the intestinal tract unbalanced for a long time, which poses a threat to animal health and even human health. direct threat. Therefore, it is imminent to accelerate the development of safe, high-quality and efficient green feed additives. Scientific research in recent years has found that the addition of live probiotic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04A23K1/16C12R1/225
Inventor 张董燕季海峰刘辉王四新王晶王雅民单达聪
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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