Lactic acid bacterium composition for treating vaginitis and preparation method thereof

A technology of lactic acid bacteria and composition, applied in the field of probiotics, can solve problems such as easy recurrence, toxic effects of lactobacilli, and unsatisfactory effects, and achieve the effect of treating vaginitis without toxic side effects

Inactive Publication Date: 2017-06-16
JIANGXI GOOD ALLY TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

② Certain spermicidal contraceptive creams have toxic effects on Lactobacillus
At present, hospitals mostly use western medicine antibiotics for treatment, but the effect is not very satisfactory, and it is easy to relapse, which brings great pain to patients

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Activation of bacteria

[0022] Screened Lactobacillus salivarius AP-32 strain resistant to 0.3% pig bile salt and acidic environment with a pH of 2.5, preservation number CCTCC NO: M2011127; Lactobacillus reuteri TE-33 strain, preservation number CCTCC NO: M2011126; Lactobacillus lysosus CT-53 strain, deposit number CCTCC NO: M2011129; Lactobacillus acidophilus F-1 strain, deposit number CCTCC NO: M2011124. Inoculate the four selected bacteria into the MRS medium at an inoculation amount of 1ml / 100ml, and culture them at 45°C for 12 hours to obtain activated Lactobacillus strains;

[0023] Among them, the formula of MRS medium is: add 10g peptone, 8g beef extract powder, 20g glucose, 4g yeast extract, 0.05g manganese sulfate tetrahydrate, 1mL Tween 80, 2g dipotassium hydrogen phosphate, 5g acetic acid in 1000mL distilled water Sodium, 0.2g magnesium sulfate, 2g diammonium citrate;

[0024] (2) Preparation of combined bacteria

[0025] Mix the four activated stra...

Embodiment 2

[0031] Screened Lactobacillus salivarius AP-32 strain resistant to 0.3% pig bile salt and acidic environment with a pH of 2.5, preservation number CCTCC NO: M2011127; Lactobacillus reuteri TE-33 strain, preservation number CCTCC NO: M2011126; Lactobacillus lysosus CT-53 strain, deposit number CCTCC NO: M2011129; Lactobacillus acidophilus F-1 strain, deposit number CCTCC NO: M2011124. Inoculate the four selected bacteria into the MRS medium at an inoculation amount of 1ml / 100ml, and culture them at 35°C for 24 hours to obtain activated Lactobacillus strains;

[0032] Among them, the formula of MRS medium is: add 10g peptone, 8g beef extract powder, 20g glucose, 5g yeast extract, 0.06g manganese sulfate tetrahydrate, 1mL Tween 80, 2g dipotassium hydrogen phosphate, 5g acetic acid in 1000mL distilled water Sodium, 0.2g magnesium sulfate, 2g diammonium citrate;

[0033] (2) Preparation of combined bacteria

[0034] Mix the four activated strains in parts by weight: 40g of Lactob...

Embodiment 3

[0040] Screened Lactobacillus salivarius AP-32 strain resistant to 0.3% pig bile salt and acidic environment with a pH of 2.5, preservation number CCTCC NO: M2011127; Lactobacillus reuteri TE-33 strain, preservation number CCTCC NO: M2011126; Lactobacillus lysosus CT-53 strain, deposit number CCTCC NO: M2011129; Lactobacillus acidophilus F-1 strain, deposit number CCTCC NO: M2011124. Inoculate the four screened bacteria into the MRS medium at an inoculation amount of 1ml / 100ml, and culture them at 42°C for 13 hours to obtain activated Lactobacillus strains;

[0041] Among them, the formula of MRS medium is: add 10g peptone, 8g beef extract powder, 20g glucose, 4g yeast extract, 0.05g manganese sulfate tetrahydrate, 1mL Tween 80, 2g dipotassium hydrogen phosphate, 5g acetic acid in 1000mL distilled water Sodium, 0.2g magnesium sulfate, 2g diammonium citrate;

[0042] (2) Preparation of combined bacteria

[0043] Mix the activated four strains in parts by weight: 35g of Lactob...

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PUM

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Abstract

The invention discloses a lactic acid bacterium composition for treating vaginitis. The lactic acid bacterium composition comprises a lactic acid bacterium combined kernel which is coated by a coating layer. The coating layer comprises, by weight, 30 to 50 parts of isomaltooligosaccharide, 30 to 50 parts of a protein substance, 5 to 10 parts of cranberry powder, 5 to 10 parts of cellulose nitrate. The lactic acid bacterium combined kernel comprises the following strains by weight: 30 to 40 parts of Lactobacillus salivarius, 20 to 30 part of Lactobacillus reuteri, 10 to 20 parts of Lactobacillus rhamnosus and 10 to 20 parts of Lactobacillus acidophilus. The invention also discloses a preparation method for the composition. The lactic acid bacterium composition provided by the invention has the advantages of substantial treatment effect on vaginitis and no toxic and side effect.

Description

technical field [0001] The invention relates to probiotics, in particular to a lactic acid bacteria composition for treating vaginitis that can significantly treat vaginitis without any toxic side effects and a preparation method thereof. Background technique [0002] Vaginitis is inflammation of the vagina. Due to the characteristics of anatomical tissue, the vagina of normal healthy women has a natural defense function against the invasion of pathogens. For example, the closure of the vaginal opening, the close contact of the front and rear walls of the vagina, the hyperplasia of vaginal epithelial cells and keratinization of surface cells under the influence of estrogen, and the balance of vaginal pH balance inhibits the reproduction of pathogens that adapt to alkaline, while the cervical mucus appears Alkaline, when the natural defense function of the vagina is destroyed, pathogens are easy to invade, which will lead to vaginal inflammation. There are many reasons for ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A61K47/42A61P15/02A61K35/744
CPCA61K35/747A61K35/744A61K47/42A61K2300/00
Inventor 刘冠环刘冠中张雪东
Owner JIANGXI GOOD ALLY TECH CO LTD
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