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Lactobacillus salivarius and application thereof

A technology of Lactobacillus salivarius, which is applied in the direction of Lactobacillus, application, bacteria, etc., can solve problems such as human health and environmental hazards, and achieve the effects of high cure rate, high adhesion, and good surface properties

Active Publication Date: 2020-03-13
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although antibiotics play a certain positive role in disease prevention and resistance, their long-term use brings harm to human health and the environment is self-evident

Method used

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  • Lactobacillus salivarius and application thereof
  • Lactobacillus salivarius and application thereof
  • Lactobacillus salivarius and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Isolation and purification of embodiment one bacterial strain

[0021] 1.1 Sample collection

[0022] Collect 120 parts of fresh feces from healthy pigs, cattle, and sheep in several farms in Pingliang City, Gansu Province, store them in 50 mL sterile centrifuge tubes, add ice packs, and transport them to the laboratory at low temperature.

[0023] 1.2 Isolation and purification of strains

[0024] Weigh 10 g of feces sample, add it to 90 mL sterile PBS (phosphate buffered saline), mix well and filter, and dilute the filtrate to 10 -4 、10 -5 、10 -6 After doubling, 0.1mL was spread on the MRS plate containing calcium, and cultured anaerobically at 37°C for 24 hours; the suspected colonies of lactic acid bacteria that produced calcium-dissolving circles on the plate were picked and stained with Gram, and the Gram-positive bacteria were picked. A single colony of a rod-shaped strain was streaked and purified on the MRS solid plate, and numbered accordingly; a total of ...

Embodiment 2

[0029] Example 2 Screening and Identification of Probiotic Candidate Beads

[0030] 2.1 Screening of highly stress-resistant lactic acid bacteria

[0031] Determination of acid resistance ability: Inoculate 1 mL of the fresh culture solution of the above 1126 strains of newly isolated lactic acid bacteria into sterile PBS with pH = 2.5. -4 、10 -5 , draw 0.1mL of the diluted solution and spread it on the MRS plate, count and measure the number of viable bacteria after anaerobic culture at 37°C for 24 hours, and calculate the survival rate with the number of viable bacteria at 0h as the control.

[0032] Determination of tolerance to bile salts: Inoculate 1 mL of the fresh culture solution of the above-mentioned 1126 isolates into sterile PBS containing 0.3% bile salts, count and determine the number of viable bacteria after enduring at 37°C for 3 hours, and count the number of viable bacteria as 0h The number was used as the control to calculate the survival rate.

[0033] T...

Embodiment 3

[0053] The biological characteristic research of embodiment Lactobacillus trisalivarius ZLp4b

[0054] 3.1 Acid tolerance study of Lactobacillus salivarius ZLp4b

[0055] The fresh ZLp4b bacterial solution was inoculated in sterile PBS with different pH values ​​(pH gradient 2.0, 2.5, 3.0, 3.5, 4.0) with 5% inoculum, and placed at 37°C for static culture for 0, 2, and 4 hours. Each treatment has 3 replicates. Take 1mL bacterial solution in 9mL PBS, mix and serially dilute to make 10 -4 、10 -5 、10 -6 For the dilution solution, take 0.1 mL of the dilution solution and spread it on the MRS solid plate for counting, and record the number of colonies on the plate after incubating at 37°C for 24 hours. The results of the tolerance of strain ZLp4b to acidic environment are shown in Table 2. ZLp4b can grow under different pH conditions. When the pH is 3.0 or above, the number of viable bacteria after 2h and 4h of cultivation is higher than 1.0×10 8 CFU / mL and basically unchanged,...

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Abstract

The invention discloses a strain of lactobacillus salivarius ZLp4b and its application in preventing and treating diarrhea of young animals. The strain has broad-spectrum bacteriostasis and can inhibit the growth of various pathogenic bacteria such as Escherichia coli, Salmonella, Staphylococcus aureus and PasteureUa multocida, etc. In addition, the strain has good in-vitro probiotic characteristics, including high gastrointestinal tolerance, high temperature tolerance and adhesion. As the lactobacillus salivarius of the invention is applied to feeding of mice and diarrhea piglets, lambs and calves, the secretion of immunoglobulin and cytokines in the serum of mice can be promoted, and the diarrhea symptoms of young animals can be cured or obviously alleviated.

Description

technical field [0001] The present invention relates to a strain of bacteria and its application. Background technique [0002] In animal husbandry, as a new type of feed additive, probiotic preparations have the characteristics of being green, healthy, non-toxic, non-residual, and not easy to produce drug resistance, and can be used as feed additives for a long time. With the continuous research on probiotics, we realize that probiotics are not only normal microorganisms that exist in the body, but also important substances that regulate various functions of the human body and animals. The application of probiotics in medical treatment, food, and breeding is increasing. more extensive. Probiotics are live microorganisms that, when ingested in sufficient quantities, confer a health effect on the host. Most of the probiotics come from the gastrointestinal tract of humans or animals, and play an important physiological role in the body. At present, the most widely used and ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P1/12A23K50/30A23K50/10A23K10/18C12R1/225
CPCC12N1/20A23K50/30A23K50/10A23K10/18A61K35/747A61P1/12C12R2001/225C12N1/205A23V2400/181
Inventor 李有全贾丹关贵全刘军龙李贺海王佳慧朱跃明刘爱红王锦明殷宏罗建勋乃比江李东平刘志杰独军政高闪电任巧云杨吉飞刘光远
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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