Lactobacillus salivarius and application thereof

A technology of Lactobacillus salivarius and bacteria agent, which is applied in the field of microorganisms, can solve the problem of incomplete restoration of tooth status, and achieve the effect of preventing dental caries, important application value, and inhibiting streptococci

Inactive Publication Date: 2017-02-15
INST OF PLA FOR DISEASE CONTROL & PREVENTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The inventors discovered this type of microorganism called Leposporium luteum (L.) Saliva) can help fight against teeth-related diseases such as plaque buildup on their surfaces. This discovery could be useful for developing better ways to protect our mouths from germs like those caused by acidic environments.

Problems solved by technology

The technical problem addressed by this patented technology relates to finding ways to reduce plaque formation caused by acid refluxing from teeth during eating while also promoting regenerative growth of streptococcidium Mutanum Bacteriophage type 1(Sporodexonella sp.) at home without causing damage through long periods afterward.

Method used

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  • Lactobacillus salivarius and application thereof
  • Lactobacillus salivarius and application thereof
  • Lactobacillus salivarius and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Embodiment 1, isolation and identification of Lactobacillus salivarius

[0068] 1. Separation

[0069] The saliva of three preschool children (all volunteers who gave informed consent to the test content) was taken, and evenly spread on the MRS solid plate, and then placed in a carbon dioxide incubator for static culture at 37°C. After culturing until the 2nd day, a single colony was produced. Pick a single colony and repeat purification more than three times. The three strains of bacteria isolated were named Bacteria LJ, Bacteria A and Bacteria B, respectively.

[0070] 2. Identification

[0071] 1. Morphological identification

[0072] The bacteria (bacteria LJ, bacteria A or bacteria B) isolated in step 1 were inoculated on the MRS solid plate, then placed in a carbon dioxide incubator, cultured at 37°C, and the morphology of a single colony was observed after 2 days.

[0073] Bacteria LJ, Bacteria A, and Bacteria B were identified as Gram-positive bacteria, sho...

Embodiment 2

[0082] Embodiment 2, the inhibitory effect of Lactobacillus salivarius to Streptococcus mutans

[0083] The experiment was repeated three times, and the average value was taken. The specific steps of each experiment are as follows:

[0084] 1. Inoculate a single colony of Lactobacillus salivarius LJ into a test tube containing 10 mL of MRS liquid medium, and then place it in a carbon dioxide incubator. After static culture at 37°C for 24 hours, the bacterial liquid of Lactobacillus salivarius LJ was obtained (OD 600nm value around 0.5).

[0085] According to the above method, the single colony of Lactobacillus salivarius LJ is replaced by the single colony of Lactobacillus salivarius A, and other steps are all unchanged, and the bacterium liquid of Lactobacillus salivarius A (OD 600nm value around 0.5).

[0086] According to the above method, the single colony of Lactobacillus salivarius LJ is replaced by the single colony of Lactobacillus salivarius B, and other steps are...

Embodiment 3

[0101] Embodiment 3, the experimental effect of Lactobacillus salivarius LJ on each bacterial genus in the oral flora

[0102] The experiments in this example were all carried out on the premise of the informed consent of the volunteers, and all signed informed consent forms. There are a variety of harmful bacteria in the oral cavity. The names of some harmful bacteria and their relationship with diseases are shown in Table 3.

[0103] table 3

[0104] Harmful bacteria name relationship with disease Streptococcus mutans cause dental caries Prevotella It is related to the occurrence and development of dental caries, periodontal disease and pulpitis Facultative diplococci infective endocarditis Actinomycetes Related to the occurrence and development of dental caries and periodontal disease ciliate fungus Root granuloma and ciliate mycotic sepsis

[0105] 1. Preparation of tablets

[0106] 1. Preparation of Tablet A

[0107] (...

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Abstract

The invention discloses a strain of lactobacillus salivarius and application thereof. The collection number of the lactobacillus salivarius LJ provided by the invention in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No.12857. Experiments prove that the lactobacillus salivarius LJ provided by the invention plays a vital role in preventing caries and/or inhibiting streptococcus and/or reducing harmful bacteria in an oral cavity and/or increasing beneficial bacteria in the oral cavity, and has an important application value.

Description

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Claims

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Application Information

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Owner INST OF PLA FOR DISEASE CONTROL & PREVENTION
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