Vitamin K2 and preparation process thereof

A vitamin, 24-hour technology, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve the problem that the preparation method cannot be applied to industrial production, and achieve the effects of low cost, high yield and high purity

Active Publication Date: 2014-02-12
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the deficiencies of the prior art, provide a kind of vitamin K2 and preparation method thereof, to solve the problem that the preparation method of the prior art cannot be applied to industrialized production

Method used

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  • Vitamin K2 and preparation process thereof
  • Vitamin K2 and preparation process thereof
  • Vitamin K2 and preparation process thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The preparation method of vitamin K2 provided by the present embodiment comprises the following steps:

[0031] (1) Take Flavobacterium HNA12-D and inoculate it in the slant medium, culture it at 30°C for 24 hours for activation, and then store it at 4°C for 2 days to obtain the activated slant-preserved bacteria of Flavobacterium HNA12-D, said The formula of the slant medium is: glycerol 8g / L, peptone 8g / L, yeast extract 1.5g / L, sodium chloride 2g / L, dipotassium hydrogen phosphate 2g / L, magnesium sulfate 0.1g / L, agar 20g / L L;

[0032] (2) Take the above-mentioned slant-preserved bacteria and inoculate them into the seed bottle medium, inoculate 0.1 cubic centimeters of the slant-preserved bacteria per 50 mL of the seed bottle culture medium, and culture them in shake flasks at 30°C for 24 hours at a speed of 120 rpm to obtain seed bottle cultures. The medium formula of the seed bottle is: glycerol 4g / L, peptone 4g / L, yeast extract 1.5g / L, sodium chloride 2g / L, dipotassi...

Embodiment 2

[0043] The preparation method of another kind of vitamin K2 provided by the present embodiment comprises the following steps

[0044] (1) Take Flavobacterium HNA12-D and inoculate it in the slant medium, culture it at 32°C for 24 hours for activation, and then store it at 4°C for 8 days to obtain the activated slant-preserved bacteria of Flavobacterium HNA12-D, said The formula of the slant medium is: glycerol 10g / L, peptone 10g / L, yeast extract 2g / L, sodium chloride 4g / L, dipotassium hydrogen phosphate 4g / L, magnesium sulfate 1g / L, agar 20g / L;

[0045] (2) Take the above-mentioned slant-preserved bacteria and inoculate them into the seed bottle medium, inoculate 0.3 cubic centimeters of slant-preserved bacteria per 50 mL of seed bottle culture medium, shake the flask at 32°C for 30 hours, and shake the flask at a speed of 240 rpm to obtain the seed bottle culture The medium formula of the seed bottle is: glycerol 6g / L, peptone 6g / L, yeast extract 2g / L, sodium chloride 3g / L, d...

Embodiment 3

[0056] The preparation method of another kind of vitamin K2 provided by the present embodiment comprises the following steps:

[0057] (1) Take Flavobacterium HNA12-D and inoculate it in the slant medium, culture it at 38°C for 24 hours for activation, and then store it at 4°C for 15 days to obtain the activated slant-preserved strain of Flavobacterium HNA12-D, said The formula of the slant medium is: glycerol 12g / L, peptone 12g / L, yeast extract 4.5g / L, sodium chloride 6g / L, dipotassium hydrogen phosphate 10g / L, magnesium sulfate 4g / L, agar 20g / L ;

[0058] (2) Take the above-mentioned slant-preserved bacteria and inoculate them into the seed bottle medium, inoculate 0.5 cubic centimeters of slant-preserved bacteria per 50 mL of seed bottle culture medium, shake the flask at 38°C for 48 hours, and shake the flask at a speed of 200 rpm to obtain the seed bottle culture The formula of the seed bottle culture medium is: glycerol 10g / L, peptone 10g / L, yeast extract 3.5g / L, sodium...

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Abstract

The invention discloses a vitamin K2 and preparation process thereof. According to the process, 1-hydroxyl-2-naphthoic acid resistant flavobacterium strain HNA12-D is fermented and cultured, and vitamin K2 efficiently metabolizes in the strain through the batch feeding of glycerin serving as the carbon source, peptone serving as the nitrogen source and precursor substances, and is extracted by using organic solvents and separated by macroporous resin, and the yield and purity of the product are improved. The total output of the vitamin K2 crude product is 200-600 mg/L after the flavobacterium strain HNA12-D of the invention is fermented and cultured for 120-144 hours, and the product purity reaches 80-99 percent after absorption chromatography; compared with the prior art, the yield output and the product purity are greatly improved, and the product can be used in industrial production.

Description

technical field [0001] The present invention relates to a vitamin K2, in particular to a vitamin K2 and a preparation method thereof. Background technique [0002] Vitamin K (VK) is a general term for a group of substances that contain a common chemical structure (2-methyl-1,4-naphthoquinone ring). According to the length of the chemical structure of the side chain connected to the 3' position of the naphthoquinone ring, VK is divided into There are two types of naturally occurring VK, vitamin K1 (phylloquinones, PK for short) and vitamin K2 (menaquinones, MK for short), both of which are fat-soluble vitamins and can be synthesized by microorganisms . The chemical structural formula of vitamin K2 is 2-methyl-3-enyl-1,4-naphthoquinone and the molecular formula is C 16 h 16 o 2 ﹒ (C 5 h 8 )n, according to the length of the isoprene side chain on C-3, vitamin K2 can be divided into 14 types, usually represented by MK-n, where n refers to the number of isoprene units on t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/66C12R1/20
Inventor 郑之明刘艳王丽王鹏赵根海刘会李哲敏吴荷芳刘红霞檀沐
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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