Composite microbial agent and application thereof
A technology of microbial inoculants and bacilli, applied in the field of agricultural microorganisms, can solve the problems of poor ability to absorb nutrients and transfer substances, inability of roots to penetrate deeply into the field, and limited expansion of surface root systems, so as to shorten the growth period and improve the resistance to bacteria. The ability to fall down, the effect of reducing chemical residues
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Embodiment 1
[0028] The preparation of embodiment 1 microbial bacterial agent
[0029] 1. Screening of functional strains
[0030] (1) Hydrogen oxidizing bacteria
[0031] Hydrogen oxidizing bacteria can use H 2 , with the participation of hydrogenase, CO assimilation 2 and H 2 Synthesize substances and perform chemoautotrophy. In the present invention, measure the H of strain with gas chromatography 2 Absorptive capacity. Purified Xanthobacterium: Xanthobacter autotrophicus, Xanthobacter flavus, Xanthobacter aminoxidans or Xanthobacter tagetidis; Pseudomonas: Phage Pseudomonas hydrogenovora, Rhodopseudomonas palustris; Rhodoparacoccus: Paracoccus sediminis, Paracoccus marcusii connected to R2A slope, etc. After the moss was sealed, the R2A slant inoculated with water was used as a control. Introduce a certain amount of H into the test tube 2 , fully mixed, in order to determine the aforementioned various hydrogen oxidizing bacteria on the H 2 Absorption, in a closed test tube,...
Embodiment 2
[0046] Embodiment 2 The impact of microbial inoculant of the present invention on rice lodging
[0047] 1. Pre-broadcast preparation
[0048] Variety selection: suitable for direct seeding in dry land, choose Longqingdao No. 2 for planting.
[0049] Land selection: flat land, the land should have under-mulch drip irrigation equipment, and the previous crops should not have phytotoxicity.
[0050] Seed fertilizer configuration: pay attention to the supplement of phosphorus and potassium, monoammonium phosphate and potassium chloride (suitable for alkaline soil), and apply farmyard manure together.
[0051] Deep loosening of the soil: Rotary plowing the land twice, after the land preparation, the soil should be crushed and leveled, and the land with phytotoxicity must be plowed in autumn and harrowed in autumn to reduce the phytotoxicity of the previous stubble.
[0052] 2. Sowing
[0053] Seed treatment: Float grains and other debris with salt water, remove the debris, was...
Embodiment 3
[0068] Embodiment 3 The impact of microbial inoculant of the present invention on rice yield
[0069] Plant Longqing Rice No. 2 according to the method of Example 2, observe and record the output of paddy rice, the result is as follows figure 1 , as shown in Table 4 and 5:
[0070] Table 4
[0071] Group (n=20) Thousand grain weight (g) Grains per spike Single panicle weight (g) Effective spike number (10 4 hm -2 )
control group 25.43±1.25 145.18±6.21 3.01±0.21 276.65±5.84 treatment group 29.55±2.33* 177.42±5.11* 3.76±0.85* 291.47±4.23*
[0072] "*" indicates the significance between the treatment group and the control group, P<0.05.
[0073] table 5
[0074]
[0075] As can be seen from Table 4, compared with the control group, the treatment group increased the thousand-grain weight of rice by 8%, the number of grains per panicle by 14%, the number of single panicles by 10%, and the effective panicle number by 2%. This sh...
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