A dwarfing planting method for Pueraria lobata

Abstract

The invention discloses a method for dwarfing kudzu vine, and relates to the field of planting technology, including: step one: raising seedlings, applying basal fertilizer, preparing land, ridging, and covering film; wherein, before raising seedlings, a mixed solution of chlormequat and carbendazim is used to soak the kudzu vine seed stem; the mixed solution also includes modified chitosan oligosaccharide, and its preparation method includes: mixing chitosan oligosaccharide and methanesulfonic acid, adding phosphorus pentoxide, stirring for 1-3h at 3-10°C, adding ether after the reaction is completed, centrifugal separation, washing, and drying to obtain; step two: transplanting and promoting seedlings; step three: wiping buds, scaffolding, and topping; step four: chemical bud control, root shearing, and topdressing; step five: vine control, and step six: harvesting. The present invention effectively shortens the nutrient transport distance from the aboveground part to the underground part on the one hand, and is convenient for using a vine control method combining artificial pruning with paclobutrazol chemical control to turn kudzu vine from vegetative growth to reproductive growth, thereby increasing kudzu vine yield.

Description

Technical Field

[0001] The invention relates to the technical field of planting, in particular to a method for dwarfing kudzu vines. Background Art

[0002] Pueraria lobata is a perennial deciduous vine of the genus Pueraria in the Fabaceae family. The roots are large. The stems and branches are covered with yellow-brown short hairs or mixed with long hard hairs. The leaves are trifoliate, with long petioles; the stipules are lanceolate and oblong, with hairs. The racemes are axillary; the bracts are ovate; the calyx is bell-shaped. The pods are oblong and flat; 10-12 cm long, 1-1.2 cm wide, densely covered with yellow-brown long hard hairs. The seeds are kidney-shaped or round. The flowering period is from June to September, and the fruiting period is from August to October.

[0003] Dwarfing is a cultivation technique that makes the plant significantly shorter than normal growth. It is mostly used in the production of fruit trees, mulberry trees and ornamental plants. Its purpose is to reduce unnecessary nutrient consumption, so as to make full use of light energy and soil fertility, bear fruit earlier, increase yield or increase ornamental effect. Therefore, how Pueraria lobata can benefit from dwarfing technology to increase root yield is a topic worth studying. Summary of the invention

[0004] The purpose of the present invention is to solve at least one of the technical problems existing in the prior art and to provide a method for dwarfing kudzu vine.

[0005] The technical solution of the present invention is as follows: a method for dwarfing kudzu vines, comprising the following steps:

[0006] Step 1: raising seedlings, applying base fertilizer, preparing the land, forming ridges, and covering with film; before raising seedlings, soaking the kudzu seed stems in a mixed solution of chlormequat chloride and carbendazim;

[0007] The mixed solution also includes modified chitosan oligosaccharide, and the preparation method thereof includes: mixing chitosan oligosaccharide and methanesulfonic acid, adding phosphorus pentoxide, stirring at 3-10°C for 1-3h, adding ether after the reaction, centrifuging, washing, and drying;

[0008] The reaction process involved:

[0009]

[0010] Step 2: Transplant and promote seedlings: Dip the lower end of the kudzu seedlings with a solution of rooting and seedling strengthening agent before transplanting, and water them with enough rooting water and apply foliar fertilizer after transplanting;

[0011] Step 3: Remove buds, build a frame, and top the plants. When the kudzu seedlings grow to 15-20 cm long, leave 1-2 strong vines per plant as the main vines, remove the excess vines, and build a 1-1.2m high vertical frame between every two kudzu seedlings. When the main vines reach the top of the frame, top the plants.

[0012] Step 4: Chemical bud control, root pruning, and topdressing; when the side branches on the main vine grow to about 30cm, use chemical vine control to control buds on the main vine within 30cm of the kudzu head, and select kudzu roots after 4-6 days. Select 1-3 tubers for each plant and leave them. Then use hole application to topdress and cover with soil immediately;

[0013] Step 5: Control vines: Prune the tops of the lateral branches of Pueraria lobata, and then immediately spray a mixed solution of 5-6g / L paclobutrazol and 1-2g / L potassium dihydrogen phosphate;

[0014] Step 6: Harvesting: Wait until the kudzu leaves turn yellow before harvesting.

[0015] Furthermore, in the step 1, the mixed solution also includes an anionic surfactant.

[0016] Furthermore, the anionic surfactant is sodium dodecylbenzene sulfonate or fatty acid salt.

[0017] Furthermore, the modified chitosan oligosaccharide accounts for 1-5wt% of the mixed solution.

[0018] Furthermore, in the step 1, the chlormequat is a 250-300 mg / L aqueous solution with an active ingredient content of 50%, and the carbendazim is a 1000-fold dilution of a 40-50 wt% carbendazim wettable powder.

[0019] Furthermore, in the step 1, applying base fertilizer specifically includes applying 100-125 kg potassium sulfate compound fertilizer and 150-200 kg organic fertilizer per mu of red soil dry land, and land preparation specifically includes plowing first and then rotary tillage, forming ridges with wide and narrow rows, and using black film for covering.

[0020] Furthermore, in step 2, the foliar fertilizer is a mixed solution of 1-2 g / L potassium dihydrogen phosphate and 5-7 g / L urea.

[0021] Furthermore, in step 4, the chemical vine control method is specifically spraying 10-20 mL / L of pendimethalin, with an active ingredient content of 330 g / L, and the dosage form is emulsifiable concentrate.

[0022] Furthermore, in step 4, topdressing is specifically: applying 10-20g of potassium sulfate fertilizer per plant.

[0023] Furthermore, in step five, pruning and spraying are performed 3 to 4 times, each time at an interval of one month.

[0024] Furthermore, step 1 is performed in January to February, step 2 is performed in early March, step 3 is performed in April to May, step 4 is performed in June, step 5 is performed in July to October, and step 6 is performed in late November to December.

[0025] The beneficial effects of the present invention are:

[0026] (1) A method for dwarfing kudzu vines of the present invention comprises adding modified chitosan oligosaccharide to a mixed solution. On the one hand, chitosan itself has a good cell activation effect. On the other hand, the phosphorylated chitosan oligosaccharide obtained after modification can stimulate the plant to produce extracellular matrix phosphoglycoprotein, improve cell proliferation ability and regulate phosphate outside the plant cells. At the same time, the amino groups on the chitosan oligosaccharide molecular structure can chelate the heavy metal ions in the plant stems, thereby reducing the influence of the heavy metals in the stems themselves on the germination rate of the stems and the subsequent dwarfing process.

[0027] (2) In the method for dwarfing kudzu vine cultivation of the present invention, an anionic surfactant is further added to the mixed solution in step 1. This is mainly because the modified chitosan oligosaccharide is a positively charged cationic alkaline amino oligosaccharide. It is combined with the anionic surfactant and enriched on the surface of the mixed solution containing chlormequat chloride and carbendazim and the anionic surfactant. On the one hand, the modified chitosan oligosaccharide has the function of activating plant cells and promoting the plant to absorb nutrients. On the other hand, it is combined with the anionic surfactant, which is more conducive to carrying chlormequat chloride and carbendazim to act as medicine on kudzu vine cultivation, which is beneficial to the subsequent dwarfing cultivation.

[0028] (3) The present invention provides a dwarfing planting method for Pueraria lobata, which adds chlormequat to treat the seed stems during the seedling raising process, inhibiting the elongation of plant cells, thereby achieving a dwarfing effect. At the same time, it cooperates with carbendazim to carry out sterilization, reducing its impact on the Pueraria lobata seed stems and improving the survival rate.

[0029] (4) The dwarfing planting method of Pueraria lobata of the present invention adopts wide and narrow row ridge-making with high efficiency, which is conducive to ventilation and light transmission, improves the lodging resistance and drought resistance of Pueraria lobata, is suitable for increasing density and yield, and is easy to operate in field farming.

[0030] (5) A dwarfing planting method for Pueraria lobata of the present invention. Currently, Pueraria lobata planting is basically ground-climbing planting and conventional elevated planting (the height of the trellis is about 2.5m). Ground-climbing planting is mostly cultivated by humans. Although the planting cost is low, it takes more than two years to harvest, the yield is low, and the annual income is low. Conventional elevated planting is generally harvested once a year, and the annual yield is much higher than that of ground-climbing planting, but the planting cost is high, especially the labor cost of trellis is high, and the cost of bamboo is also high. In the later stage of planting, the kudzu vine biomass is large and easy to slide. Pueraria lobata dwarfing planting is dwarfed on the basis of the original conventional elevated planting. The height of the trellis is 1-1.2m. The trellis efficiency is high and the bamboo cost is saved. In the later stage, when the kudzu vine biomass is large, it is not easy to slide.

[0031] (6) The present invention provides a dwarfing planting method for Pueraria lobata. On the one hand, dwarfing planting effectively shortens the nutrient transport distance from the above-ground part to the underground part. On the other hand, it facilitates the use of a vine control method that combines manual pruning with paclobutrazol chemical control to make Pueraria lobata switch from vegetative growth to reproductive growth, thereby increasing Pueraria lobata yield.

[0032] (7) In the method for dwarfing kudzu vine of the present invention, pruning is added before spraying paclobutrazol, and potassium dihydrogen phosphate is added to paclobutrazol to enhance the control effect of paclobutrazol.

[0033] (8) In the dwarfing planting method of Pueraria lobata of the present invention, topdressing is mainly controlled after the Pueraria lobata roots are exposed and pruned, and no nitrogen fertilizer is applied, but only 20g / plant of potassium sulfate fertilizer is applied, thereby controlling the nutritional growth of the plants and achieving a dwarfing effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 The dwarfing image of Example 1 (left) and the conventional image without the dwarfing technology of the present invention (right). DETAILED DESCRIPTION

[0035] This section will describe in detail the specific embodiments of the present invention. The preferred embodiments of the present invention are shown in the accompanying drawings. The purpose of the accompanying drawings is to supplement the description of the text part of the specification with graphics, so that people can intuitively and vividly understand each technical feature and the overall technical solution of the present invention, but it cannot be understood as a limitation on the scope of protection of the present invention.

[0036] The technical solution of the present invention is further described below with reference to specific embodiments. Figure 1 The flow chart of the experiment was used;

[0037] Example 1

[0038] Step 1: From January to February, seedling raising, basal fertilizer application, land preparation, ridge formation, and film covering: In early January, soak the kudzu seed stems for 6 hours in a mixed solution of 270mg / L chlormequat (aqueous solution, with an active ingredient content of 50%) and 50% carbendazim wettable powder 1000 times diluted carbendazim, modified chitosan oligosaccharide, and sodium dodecylbenzene sulfonate in a mass ratio of 3:1:1:0.5, and then cut and raise seedlings. Apply 115kg of potassium sulfate compound fertilizer and 180kg of organic fertilizer (a mixture of one or more of rapeseed cake, rice husk ash, or commercial organic fertilizer) per mu, first plow and then rotary tillage, wide and narrow row ridge formation (1.5m wide row, 1.0m narrow row), and the black film used for film covering is 2.0m wide;

[0039] The preparation method of modified chitosan oligosaccharide is as follows: chitosan oligosaccharide and methanesulfonic acid are mixed, phosphorus pentoxide is added, and the mixture is stirred at 5°C for 2 hours. After the reaction is completed, ether is added, and the mixture is centrifuged, washed, and dried to obtain the modified chitosan oligosaccharide.

[0040] The amount of modified oligochitosan added is 2wt% of the mixed solution; the amount of sodium dodecylbenzene sulfonate added is 0.5wt% of the mixed solution;

[0041] Step 2: In early March, transplant and promote seedlings: the spacing between kudzu seedlings is 45 cm. Before transplanting, dip the lower end of the kudzu seedlings with 4g / L rooting and seedling strengthening agent solution. After transplanting, water them in time to establish roots. After 10-15 days of transplanting, apply foliar fertilizer three times with an interval of 7 days. The foliar fertilizer is a mixed solution of 2g / L potassium dihydrogen phosphate and 7g / L urea.

[0042] Step 3: April to May, remove buds, build frames, and top the plants: When the kudzu seedlings grow to 20 cm long, leave one strong vine as the main vine for each plant, remove the excess vines, and insert a bamboo pole about 1.2m long and 3cm in diameter between every two kudzu plants for vertical frame building. When the main vine grows to the top of the bamboo pole, top the plants;

[0043] Step 4: In June, chemical bud control, root pruning, and topdressing: When the side branches on the upper part of the main vine grow to about 30cm, the main vine within 30cm from the kudzu head is controlled by chemical bud control, that is, spraying 20mL / L of pendimethalin (active ingredient content 330g / L, dosage form is emulsifiable concentrate), and after an interval of 4 to 6 days, the root selection of kudzu is carried out, and one tuber is selected for each plant. Then, topdressing is carried out by hole application, and about 20g of potassium sulfate fertilizer is applied to each plant, and the soil is covered immediately;

[0044] Step 5: July to October, control vines: Use motorized hedge shears to trim the tops of the lateral branches of Pueraria lobata from July to October, and then immediately spray a mixed solution of 6g / L paclobutrazol and 2g / L potassium dihydrogen phosphate. Trim and spray three times, with an interval of one month each.

[0045] Step 6: Harvest from late November to December: Wait until the leaves of Pueraria lobata turn yellow before harvesting.

[0046] Example 2

[0047] Step 1: From January to February, seedling raising, basal fertilizer application, land preparation, ridge formation, and film covering: In early January, soak the kudzu seed stems for 5 to 6 hours with a mixed solution of 300 mg / L chlormequat (aqueous solution, with an active ingredient content of 50%) and 50% carbendazim wettable powder 1000 times diluted carbendazim, modified chitosan oligosaccharide, and sodium dodecylbenzene sulfonate in a mass ratio of 3:1:1:1, and then cut and raise seedlings. Apply 125 kg of potassium sulfate compound fertilizer and 180 kg of organic fertilizer (a mixture of one or more of rapeseed cake, rice husk ash, or commercial organic fertilizer) per mu, first plow and then rotary tillage, wide and narrow row ridge formation (1.5 m wide row, 1.0 m narrow row), and the black film used for film covering is 2.0 m wide;

[0048] The preparation method of modified chitosan oligosaccharide is as follows: chitosan oligosaccharide and methanesulfonic acid are mixed, phosphorus pentoxide is added, and the mixture is stirred at 10°C for 1.5 hours. After the reaction is completed, ether is added, and the mixture is centrifuged, washed, and dried to obtain the modified chitosan oligosaccharide.

[0049] The amount of modified oligochitosan added is 3wt% of the mixed solution; the amount of sodium dodecylbenzene sulfonate added is 0.8wt% of the mixed solution;

[0050] Step 2: In early March, transplant and promote seedlings: the spacing between kudzu seedlings is 41cm. Before transplanting, dip the lower end of the kudzu seedlings with 4g / L rooting and seedling strengthening agent solution. After transplanting, water them in time to ensure that they will take root. After 10-15 days of transplanting, apply foliar fertilizer 2-3 times at intervals of 6 days. The foliar fertilizer is a mixed solution of 2g / L potassium dihydrogen phosphate and 8g / L urea.

[0051] Step 3: From April to May, remove buds, build frames, and top the plants: When the kudzu seedlings grow to 15-20 cm long, leave 1-2 strong vines per plant as the main vines, remove the excess vines, and insert a bamboo pole about 1.2m long and 3cm in diameter between every two kudzu plants for vertical frame building. When the main vine grows to the top of the bamboo pole, top the plants;

[0052] Step 4: In June, chemical bud control, root pruning and topdressing: When the side branches on the upper part of the main vine grow to about 30cm, the main vine within 30cm from the kudzu head is controlled by chemical vine control, that is, spraying 20mL / L of pendimethalin (active ingredient content 330g / L, dosage form is emulsifiable concentrate), and after an interval of 5 days, the root selection of kudzu is carried out, and 2 tubers are selected for each plant. Then, topdressing is carried out by hole application, and about 20g of potassium sulfate fertilizer is applied to each plant, and the soil is covered immediately;

[0053] Step 5: July to October, control vines: Use motorized hedge shears to trim the tops of the lateral branches of Pueraria lobata from July to October, and then immediately spray a mixed solution of 5g / L paclobutrazol and 3g / L potassium dihydrogen phosphate. Trim and spray three times, with an interval of one month each.

[0054] Step 6: Harvest from late November to December: Wait until the leaves of Pueraria lobata turn yellow before harvesting.

[0055] Example 3

[0056] Step 1: From January to February, seedling raising, basal fertilizer application, land preparation, ridge formation, and film covering: In early January, soak the kudzu seed stems for 6 hours with a mixed solution of 260mg / L chlormequat (aqueous solution, with an active ingredient content of 50%) and 50% carbendazim wettable powder 1000 times diluted carbendazim, modified chitosan oligosaccharide, and sodium dodecylbenzene sulfonate in a mass ratio of 3:1:1:1, and then cut and raise seedlings. Apply 115kg of potassium sulfate compound fertilizer and 180kg of organic fertilizer (a mixture of one or more of rapeseed cake, rice husk ash, or commercial organic fertilizer) per mu, first plow and then rotary tillage, wide and narrow row ridge formation (1.5m wide row, 1.0m narrow row), and the black film used for film covering is 2.0m wide;

[0057] The preparation method of modified chitosan oligosaccharide is as follows: chitosan oligosaccharide and methanesulfonic acid are mixed, phosphorus pentoxide is added, and the mixture is stirred at 6°C for 2 hours. After the reaction is completed, ether is added, and the mixture is centrifuged, washed, and dried to obtain the modified chitosan oligosaccharide.

[0058] The amount of modified oligochitosan added is 2 wt% of the mixed solution; the amount of sodium dodecylbenzene sulfonate added is 0.9 wt% of the mixed solution;

[0059] Step 2: In early March, transplant and promote seedlings: the spacing between kudzu seedlings is 41cm to 48cm. Before transplanting, dip the lower end of the kudzu seedlings with 4g / L rooting and seedling strengthening agent solution. After transplanting, water them in time to ensure that they will take root. After 12 days of transplanting, apply foliar fertilizer twice, with an interval of 6 days. The foliar fertilizer is a mixed solution of 2g / L potassium dihydrogen phosphate and 7g / L urea.

[0060] Step 3: April to May, remove buds, build frames, and top the plants: When the kudzu seedlings grow to 17 cm long, leave 1 to 2 strong vines as the main vines for each plant, remove the excess vines, and insert a bamboo pole about 1.2m long and 3cm in diameter between every two kudzu plants for vertical frame building. When the main vine grows to the top of the bamboo pole, top the plants;

[0061] Step 4: In June, chemical bud control, root pruning and topdressing: When the side branches on the upper part of the main vine grow to about 30cm, the main vine within 30cm from the kudzu head is controlled by chemical vine control, that is, 18mL / L of pendimethalin (active ingredient content 330g / L, dosage form is emulsifiable concentrate) is sprayed, and after an interval of 5 days, the root selection of kudzu is carried out, and 2 tubers are selected for each plant. Then, topdressing is carried out by hole application, and 18g of potassium sulfate fertilizer is applied to each plant, and the soil is covered immediately;

[0062] Step 5: July to October, control vines: Use motorized hedge shears to trim the tops of the lateral branches of Pueraria lobata from July to October, and then immediately spray a mixed solution of 6g / L paclobutrazol and 2g / L potassium dihydrogen phosphate. Trim and spray 4 times, with an interval of 1 month each time.

[0063] Step 6: Harvest from late November to December: Wait until the leaves of Pueraria lobata turn yellow before harvesting.

[0064] Example 4

[0065] Step 1: From January to February, seedling raising, basal fertilizer application, land preparation, ridge formation, and film covering: In early January, soak the kudzu seed stems for 5 hours in a mixed solution of 300mg / L chlormequat (aqueous solution, with an active ingredient content of 50%) and 50% carbendazim wettable powder 1000 times diluted carbendazim, modified chitosan oligosaccharide, and sodium dodecylbenzene sulfonate in a mass ratio of 3:1:1:1, and then cut and raise seedlings. Apply 125kg of potassium sulfate compound fertilizer and 200kg of organic fertilizer (rice husk ash) per mu, first plow and then rotary tillage, wide and narrow row ridge formation (wide row 1.5m, narrow row 1.0m), and the black film used for film covering is 2.0m wide;

[0066] The preparation method of modified chitosan oligosaccharide is as follows: chitosan oligosaccharide and methanesulfonic acid are mixed, phosphorus pentoxide is added, and the mixture is stirred at 4°C for 3 hours. After the reaction is completed, ether is added, and the mixture is centrifuged, washed, and dried to obtain the modified chitosan oligosaccharide.

[0067] The amount of modified oligochitosan added is 5wt% of the mixed solution; the amount of sodium dodecylbenzene sulfonate added is 1wt% of the mixed solution;

[0068] Step 2: In early March, transplant and promote seedlings: the spacing between kudzu seedlings is 48cm. Before transplanting, dip the lower end of the kudzu seedlings with 4g / L rooting and seedling strengthening agent solution. After transplanting, water them in time to establish roots. After 15 days of transplanting, apply foliar fertilizer 3 times with an interval of 6 days. The foliar fertilizer is a mixed solution of 2g / L potassium dihydrogen phosphate and 7g / L urea.

[0069] Step 3: April to May, remove buds, build frames, and top the plants: When the kudzu seedlings grow to 15-20 cm long, leave two strong vines on each plant as the main vines, remove the excess vines, and insert a bamboo pole about 1.2m long and 3cm in diameter between every two kudzu plants for vertical frame building. When the main vine grows to the top of the bamboo pole, top the plants;

[0070] Step 4: In June, chemical bud control, root pruning and topdressing: When the side branches on the upper part of the main vine grow to about 30cm, the main vine within 30cm from the kudzu head is controlled by chemical vine control, that is, spraying 20mL / L of pendimethalin (active ingredient content 330g / L, dosage form is emulsifiable concentrate), and after an interval of 6 days, the root selection of kudzu is carried out, and 3 tubers are selected for each plant. Then, topdressing is carried out by hole application, and about 20g of potassium sulfate fertilizer is applied to each plant, and the soil is covered immediately;

[0071] Step 5: July to October, control vines: Use motorized hedge shears to trim the tops of the lateral branches of Pueraria lobata from July to October, and then immediately spray a mixed solution of 6g / L paclobutrazol and 2g / L potassium dihydrogen phosphate. Trim and spray 4 times, with an interval of 1 month each time.

[0072] Step 6: Harvest from late November to December: Wait until the leaves of Pueraria lobata turn yellow before harvesting.

[0073] Example 5

[0074] Step 1: From January to February, seedling raising, basal fertilizer application, land preparation, ridge formation, and film covering: In early January, soak the kudzu seed stems for 6 hours in a mixed solution of 250-300 mg / L chlormequat (aqueous solution, with an active ingredient content of 50%) and 50% carbendazim wettable powder 1000 times diluted carbendazim, modified chitosan oligosaccharide, and sodium dodecylbenzene sulfonate in a mass ratio of 3:0.5:1:1, and then cut and raise seedlings. Apply 100 kg of potassium sulfate compound fertilizer and 200 kg of organic fertilizer (rapeseed cake) per mu, first plow and then rotary tillage, wide and narrow row ridge formation (1.5 m wide row, 1.0 m narrow row), and the black film used for film covering is 2.0 m wide;

[0075] The preparation method of modified chitosan oligosaccharide is as follows: chitosan oligosaccharide and methanesulfonic acid are mixed, phosphorus pentoxide is added, and the mixture is stirred at 10°C for 1 hour. After the reaction is completed, ether is added, and the mixture is centrifuged, washed, and dried to obtain the modified chitosan oligosaccharide.

[0076] The amount of modified oligochitosan added is 5wt% of the mixed solution; the amount of sodium dodecylbenzene sulfonate added is 1.5wt% of the mixed solution;

[0077] Step 2: In early March, transplant and promote seedlings: the spacing between kudzu seedlings is 41cm. Before transplanting, dip the lower end of the kudzu seedlings with 4g / L rooting and seedling strengthening agent solution. After transplanting, water them in time to ensure that they will take root. 15 days after transplanting, apply foliar fertilizer 3 times with an interval of 7 days. The foliar fertilizer is a mixed solution of 2g / L potassium dihydrogen phosphate and 7g / L urea.

[0078] Step 3: April to May, remove buds, build frames, and top the plants: When the kudzu seedlings grow to 15 cm long, leave two strong vines on each plant as the main vines, remove the excess vines, and insert a bamboo pole about 1.2m long and 3cm in diameter between every two kudzu plants for vertical frame building. When the main vine grows to the top of the bamboo pole, top the plants;

[0079] Step 4: In June, chemical bud control, root pruning and topdressing: When the side branches on the upper part of the main vine grow to about 30cm, the main vine within 30cm from the kudzu head is controlled by chemical vine control, that is, spraying 20mL / L of pendimethalin (active ingredient content 330g / L, dosage form is emulsifiable concentrate), and after an interval of 6 days, the root selection of kudzu is carried out, and 2 tubers are selected for each plant. Then, topdressing is carried out by hole application, and about 20g of potassium sulfate fertilizer is applied to each plant, and the soil is covered immediately;

[0080] Step 5: July to October, control vines: Use motorized hedge shears to trim the tops of the lateral branches of Pueraria lobata from July to October, and then immediately spray a mixed solution of 6g / L paclobutrazol and 2g / L potassium dihydrogen phosphate. Trim and spray three times, with an interval of one month each.

[0081] Step 6: Harvest from late November to December: Wait until the leaves of Pueraria lobata turn yellow before harvesting.

[0082] Comparative Example 1

[0083] Different from Example 1, the mixed solution in step 1 does not contain modified oligochitosan and anionic surfactant.

[0084] Comparative Example 2

[0085] The difference from Example 1 is that unmodified oligochitosan and anionic surfactant are added to the mixed solution in step 1.

[0086] Comparative Example 3

[0087] Different from Example 1, no anionic surfactant is added to the mixed solution in step 1.

[0088] Comparative Example 4

[0089] Different from Example 1, no mixed solution treatment is used in step 1.

[0090] The dynamic changes of the height of dwarfing kudzu vines in the embodiment and the comparative example were observed, as shown in Table 1:

[0091] Table 1 Dynamic table of dwarfing planting of Pueraria lobata in the examples and comparative examples

[0092]

[0093]

[0094] It was also found that the stem thickness of the Pueraria lobata in the embodiment did not change much before topping, but gradually became thicker after topping, indicating that it played an obvious dwarfing role.

[0095] The thickness of the harvest period is 1.0-1.4 cm. From bottom to top, the internode distances of the first to eighth nodes of Example 1 are 2.58 cm, 2.93 cm, 3.32 cm, 4.07 cm, 4.76 cm, 5.59 cm, 6.58 cm, and 7.34 cm, respectively. Figure 1 (left), while the actual picture of the harvest period without the dwarfing technology of Example 1 is referenced Figure 1 (right);

[0096] In addition, the cell division index (division index = number of cells in the division phase / total number of cells × 100%) of the kudzu seed stems was tested after the embodiment and the comparative example were treated with the mixed solution of step 1. The test method is as follows. The test results are shown in Table 2.

[0097] The test method is as follows: digest the cells and transfer the cell suspension to a culture dish containing a coverslip. Culture in a CO2 incubator for 48 hours to allow the cells to grow on the coverslip. Take out the coverslip and follow the following sequence: rinse with PBS for 3 minutes, fix in a fixative solution of methanol: glacial acetic acid = 3:1 for 30 minutes, and stain with Giemsa solution for 10 minutes. Finally, rinse with tap water. After the coverslip is dried, turn it upside down on a slide and examine it under a microscope.

[0098] Table 2 Cell division index of examples and comparative examples

[0099] Sample Cell division index Example 1 0.21 Example 2 0.22 Example 3 0.25 Example 4 0.23 Example 5 0.22 Comparative Example 1 0.09 Comparative Example 2 0.13 Comparative Example 3 0.15 Comparative Example 4 0.05

[0100] It can be seen from Table 1 and Table 2 that the dwarfing planting technology of the present invention can dwarf the kudzu vine well, make the stem thicker, and increase the yield. The dwarfing effect of the embodiment is better than that of the comparative example. The main reason may be that, from the analysis of comparative examples 1 and 2, it can be seen that modified chitosan oligosaccharide is added to the mixed solution in step 1 in the embodiment. On the one hand, chitosan itself has a good cell activation effect. On the other hand, the phosphorylated chitosan oligosaccharide obtained after modification can stimulate the plant to produce extracellular matrix phosphoglycoprotein, improve cell proliferation ability and phosphate regulation outside plant cells, and at the same time, the amino group thereon can complex the heavy metal ions of the plant seed stem, reducing the influence of the heavy metals in the seed stem itself on the subsequent dwarfing process. From the analysis of comparative example 3, it can be seen that anionic surfactant is also added to the mixed solution, mainly because the modified chitosan oligosaccharide is a positively charged cationic alkaline amino oligosaccharide, combined with anionic surfactant, enriched on the surface of the mixed solution containing chlormequat and carbendazim and anionic surfactant, on the one hand, chitosan oligosaccharide has the effect of activating plant cells and promoting plant absorption of nutrients, on the other hand, the anionic surfactant used in combination is more conducive to carrying chlormequat and carbendazim to act on the kudzu seeds, which is conducive to subsequent dwarfing planting. From the analysis of comparative example 4, it can be seen that a mixed solution containing chlormequat and carbendazim is used for treatment in the embodiment, wherein chlormequat inhibits the elongation of plant cells, thereby achieving a dwarfing effect, and simultaneously cooperates with carbendazim for sterilization, reducing its effect on the kudzu seeds and improving the survival rate.

[0101] Under the premise that no conflict occurs, those skilled in the art may freely combine and superimpose the above-mentioned additional technical features.

[0102] The above description is only a preferred embodiment of the present invention. Any technical solution that achieves the purpose of the present invention by substantially the same means belongs to the protection scope of the present invention.

Claims

1. A method for dwarfing kudzu vine, characterized in that: The following steps are involved: Step 1: raising seedlings, applying base fertilizer, preparing the land, forming ridges, and covering with film; before raising seedlings, soaking the kudzu seed stems in a mixed solution of chlormequat chloride and carbendazim; The mixed solution also includes modified chitosan oligosaccharide, and the preparation method thereof includes: mixing chitosan oligosaccharide and methanesulfonic acid, adding phosphorus pentoxide, stirring at 3-10°C for 1-3h, adding ether after the reaction, centrifuging, washing, and drying; Step 2: Transplant and promote seedlings: Dip the lower end of the kudzu seedlings with a solution of rooting and seedling strengthening agent before transplanting, and water them with enough rooting water and apply foliar fertilizer after transplanting; Step 3: Remove buds, build frames, and top the plants. When the kudzu seedlings grow to 15-20 cm long, leave 1-2 strong vines per plant as the main vines, remove the excess vines, and build a 1-1.2m high vertical frame between every two kudzu seedlings. When the main vines reach the top of the frame, top the plants. Step 4: Chemical bud control, root pruning, and topdressing; when the side branches on the upper part of the main vine grow to about 30cm, the main vine within 30cm from the kudzu head is controlled by chemical vine control, and after an interval of 4 to 6 days, the root is exposed and kudzu is selected. 1 to 3 tubers are selected for each plant, and then topdressing is carried out by hole application, and soil is covered immediately; Step 5: Control vines: Prune the tops of the lateral branches of Pueraria lobata, and then immediately spray a mixed solution of 5-6g / L paclobutrazol and 1-2g / L potassium dihydrogen phosphate; Step 6: Harvest: Harvest after the leaves of Pueraria lobata turn yellow; In the step 1, the mixed solution also includes an anionic surfactant; The anionic surfactant is sodium dodecylbenzene sulfonate or fatty acid salt.

2. The method for dwarfing kudzu vine according to claim 1, characterized in that: The modified chitosan oligosaccharide accounts for 1-5wt% of the mixed solution.

3. The method for dwarfing kudzu vine according to claim 1, characterized in that: In the step 1, the chlormequat is an aqueous solution of 250-300 mg / L, with an active ingredient content of 50 wt%, and the carbendazim is a 1000-fold dilution of a carbendazim wettable powder with a concentration of 40-50 wt%.

4. The method for dwarfing kudzu vine according to claim 1, characterized in that: In the step 1, applying base fertilizer specifically includes applying 100-125 kg potassium sulfate compound fertilizer and 150-200 kg organic fertilizer per mu of red soil dry land, and preparing the land specifically includes plowing first and then rotary tillage, forming ridges with wide and narrow rows, and using black film for covering.

5. The method for dwarfing kudzu vine according to claim 1, characterized in that: In the step 2, the foliar fertilizer is a mixed solution of 1-2 g / L potassium dihydrogen phosphate and 5-7 g / L urea.

6. The method for dwarfing kudzu vine according to claim 1, characterized in that: In the step 4, the chemical vine control method is specifically spraying 10-20 mL / L of pendimethalin, with an active ingredient content of 330 g / L, and the dosage form is emulsifiable concentrate.

7. The method for dwarfing kudzu vine according to claim 1, characterized in that: In step 4, topdressing is specifically: applying 10-20g of potassium sulfate fertilizer per plant.

8. The method for dwarfing kudzu vine according to claim 1, characterized in that: In the step 5, pruning and spraying are performed 3 to 4 times, each time at an interval of 1 month.

Images