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Aflatoxin-removed feed prepared from kitchen waste and production technology of aflatoxin-removed feed

A technology for aflatoxin and kitchen waste, applied in animal feed, animal feed, food processing, etc., can solve the problems of reduced economic and practical value, large feed safety problems, accumulation of aflatoxin, etc., and reduce the residence time. , reduce the content, accelerate the effect of intestinal peristalsis

Inactive Publication Date: 2018-05-11
季瑶忆
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most feeds are produced by microbial fermentation of food waste. Under the action of mold, it is easy to cause a large amount of aflatoxin to accumulate in the feed. AFB1 can reach up to 80μg / kg, which is four times higher than the national standard. Therefore, how to produce Aflatoxin-free feed is an urgent problem to be solved
[0003] The patent application number is CN201410125531.3, which uses cutinase, neutral protease, polyphenol oxidase, hemicellulase, glucose oxidase, Polygonum cuspidatum extract, vitamin A and vitamin D to prepare aflatoxin Detoxification enzyme, the detoxification enzyme can not only degrade aflatoxin B1 in the feed, but also hydrolyze macromolecular substances, but the enzyme has strict requirements on the environment, and when the conditions are not suitable, it will be volatile and inactivated, and the cost of the enzyme is relatively high. and reduced practical value
The patent with the application number CN201610602469.1 uses a variety of microorganisms to ferment food waste, but does not degrade aflatoxin and toxic substances. Therefore, the feed produced by this method has a large safety problem

Method used

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  • Aflatoxin-removed feed prepared from kitchen waste and production technology of aflatoxin-removed feed
  • Aflatoxin-removed feed prepared from kitchen waste and production technology of aflatoxin-removed feed
  • Aflatoxin-removed feed prepared from kitchen waste and production technology of aflatoxin-removed feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Step 1: Pre-treat the food waste so that the water content is ≦40% and the protein content is ≧25%;

[0026] Step 2: crush 40% of kitchen waste and 30% of wheat bran, and pass through a 25-mesh sieve to obtain a fermentation substrate;

[0027] Step 3: Mixing Bacillus subtilis, Lactobacillus casei and Flavobacterium aurantiacus according to the weight ratio of 1.5:2:1.8 to obtain mold-removing bacteria;

[0028] Step 4: At 35°C, after passing the air through the microporous ultrafiltration membrane, continuously pass it into the fermentation substrate, and mix it with 4% citric acid to adjust the pH to 6.3, add 10% mold removal bacteria, and stir for 30 minutes every 2 days , fermented for 10 days to obtain roughage;

[0029] Step 5: After immersing 11-15 parts of attapulgite in dilute hydrochloric acid with a concentration of 0.5 mol / L for 3 hours, the modified attapulgite is obtained, and mixed with 6 parts of glucomannan, 12 parts of sorbic acid, 8 parts Sodium pro...

Embodiment 2

[0032] The operation is the same as in Example 1, wherein the mass percentages of food waste and wheat bran in step 2 are 50% and 45% respectively, and passed through a 37.5 mesh sieve; The temperature is 37°C, the pH is 6.5, the mass percentages of citric acid and mold-removing bacteria are 6% and 12.5% ​​respectively, and fermentation is carried out for 12 days; The parts by weight of the components are 9 parts, 13 parts, 14 parts, 11 parts and 14 parts; in the step 6, the mass percentages of the mildew-removing agent, tea polyphenols and trace elements are 10%, 4.5%, and 0.2% respectively.

Embodiment 3

[0034] The operation is the same as in Example 1, wherein the mass percentages of food waste and wheat bran in step 2 are 60% and 37.5% respectively, and passed through a 50-mesh sieve; The temperature is 38°C, the pH is 6.8, the mass percentages of citric acid and mold-removing bacteria are 8% and 15% respectively, and fermentation is carried out for 15 days; in step 5, the concentration of dilute hydrochloric acid is 1mol / L, the soaking time is 6h, and the weight of each component of the mold-removing agent is The number of parts is 12 parts, 15 parts, 16 parts, 14 parts and 15 parts; in the step 6, the mass percentages of mold remover, tea polyphenols and trace elements are 12%, 6%, 0.3% respectively.

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Abstract

The invention discloses an aflatoxin-removed feed prepared from kitchen waste and a production technology of the aflatoxin-removed feed. The aflatoxin-removed feed is prepared from the following raw materials in percentage by mass: 40-60% of kitchen waste, 30-45% of wheat bran, 4-8% of citric acid, 3-6% of tea polyphenols, 0.1-0.3% of trace elements, 8-12% of a mildew removing agent, and 10-15% ofmildew removing bacteria, wherein the mildew removing agent comprises the following components in parts by weight: 6-12 parts of glucomannan, 11-15 parts of modified attapulgite, 12-16 parts of sorbic acid, 8-14 parts of sodium propionate, and 13-15 parts of quercetin; and the mildew removing bacteria are prepared by mixing bacillus subtilis with lactobacillus casei and orange flavobacterium in the weight ratio of the bacillus subtilis to the lactobacillus casei to the orange flavobacterium being (1.5-2.5) to (2-3) to (1.8-2.6). According to the aflatoxin-removed feed, the growth and the propagation of mould can be effectively restrained, the metabolic pathway generating AFB1 can be restrained, the removing rate of AFB1 can achieve 91.7%, the removing rate of AFB2 can achieve 83.5%, the removing rate of AFG1 can achieve 61.8%, the removing rate of AFG2 can achieve 86.2%, the content of aflatoxins in the feed can be obviously reduced, and the safety and the feasibility for treating thekitchen waste for the feed can be improved.

Description

technical field [0001] The invention belongs to the technical field of turning kitchen waste into feed, and in particular relates to aflatoxin-free feed prepared from kitchen waste and a production process thereof. Background technique [0002] Aflatoxin, classified as a Class 1 carcinogen by the World Health Organization, is 68 times more toxic than arsenic and 10 times more toxic than potassium cyanide. It is extremely destructive to liver tissue. AFB1 is one of the known aflatoxins The most carcinogenic one. At present, most feeds are produced by microbial fermentation of food waste. Under the action of mold, it is easy to cause a large amount of aflatoxin to accumulate in the feed. AFB1 can reach up to 80μg / kg, which is four times higher than the national standard. Therefore, how to produce Aflatoxin-free feed is an urgent problem to be solved. [0003] The patent application number is CN201410125531.3, which uses cutinase, neutral protease, polyphenol oxidase, hemicel...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/26A23K10/37A23K20/105A23K20/10A23K20/20A23L5/20
CPCA23K10/12A23K10/26A23K10/37A23K20/10A23K20/105A23K20/30A23L5/27A23L5/273A23L5/28Y02P60/87
Inventor 季瑶忆
Owner 季瑶忆
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