Culture method of biological cleaning strains in low-concentration volatile mixed organic waste gas

An organic waste gas, biological purification technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems of weak purification ability, difficult to control the growth balance of various microorganisms, etc., and achieve high-efficiency degradation. Effect

Active Publication Date: 2009-09-23
GUANGZHOU HUANFA ENVIRONMENTAL ENG CO LTD
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AI Technical Summary

Problems solved by technology

However, the growth balance between multiple microorganisms is difficult to control, so it is easy to cause only a single type of microorganism to grow dominant
Therefore, the purification ability of the biological purification system for industrial waste gas of various organic compounds mixed together is weak

Method used

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  • Culture method of biological cleaning strains in low-concentration volatile mixed organic waste gas
  • Culture method of biological cleaning strains in low-concentration volatile mixed organic waste gas

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Embodiment Construction

[0020] The cultivation operation of the low-concentration volatile and volatile mixed organic waste gas biological purification strains is carried out at normal temperature and pressure, and an aerobic shaking bottle cultivation method is adopted. It includes three steps of strain preparation 1, inoculation operation and cultivation and acclimatization.

[0021] (1) Prepare strain one. It can be divided into three sub-steps.

[0022] (a) Prepare the liquid culture medium of strain one.

[0023] Take 5.0 mL of phosphate buffer, MgSO 4 Solution 3.0mL, CaCl 2 Solution 1.0mL, FeCl 3 Mix 1.0mL of solution and 1.0mL of trace element solution, add distilled water to dilute to 1.0L, and adjust the pH value to 7.0-7.2. After high-pressure steam sterilization and aseptic subpackaging, 0.5g of liquid xylene and 0.5g of diesel oil are added as the only carbon source and energy source, and sealed for storage.

[0024] Wherein, the composition of each solution of the liquid culture me...

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Abstract

The invention relates to a culture method of biological cleaning strains in low-concentration volatile mixed organic waste gas. The method comprises the following steps: a), culturing a first strain; b), carrying out the combined inoculation of the first strain and a second strain and using tap water to prepare the sludge concentration of the mixed strains; and c), using mixed organic matter in the waste gas as a substrate to be cast into an insition culture medium so as to culture and train insitions. The first strain is prepared by culturing and training from activated mud taken from a printing and dyeing sewage treatment plant and is a mixed strain of flavobacterium and Bacillus cereus, and the second strain is prepared by collecting activated mud collected from a secondary sedimentation tank of a petrochemical sewage treatment plant and is a mixed strain comprising the flavobacterium, the Bacillus cereus, xylose oxidase Achromobacter and Pseudomonas stutzeri. The method facilitates the balanced growth of various cultured microorganisms and can simultaneously decompound various target configuration compounds at high efficiency.

Description

technical field [0001] The invention belongs to the technical field of biological strain cultivation, and in particular relates to a cultivation method for biological purification strains of low-concentration volatile mixed organic waste gas. Background technique [0002] Air pollution is one of the most prominent environmental problems at present. The industrial waste gas of volatile organic compounds is the most extensive and most diverse air pollutant except dust. Biological purification of low-concentration industrial waste gas is a new method of industrial waste gas purification, and its extensive research and application has only about 20 years of history. This method uses the characteristics of microorganisms that have strong and fast adaptability to pollutants, and domesticates them so that microorganisms can use target pollutants as carbon sources and energy sources to convert pollutants into harmless, simple Substances (such as carbon dioxide, water, etc.). Micr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B01D53/84C12R1/085C12R1/025C12R1/38C12R1/20
CPCY02A50/20
Inventor 左红影林晓生程汉林
Owner GUANGZHOU HUANFA ENVIRONMENTAL ENG CO LTD
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