Preparation method of lycopene

Abstract

The invention discloses a method for preparing lycopene. The crtI gene of Erwinia h erbicola is amplified through the PCR technology and is connected with an expression vector pRKR5 containing strong promoters Puc to construct an expression plasmid pRKR5-crtI which is inducted into a mutant TC72 of photosynthetic bacteria Rhodobacter s phaeroides in the mode of conjugal transfer. Due to the high-efficiency expression of the promoters Puc under the anaerobic condition, the adjustment and control on oxygen concentration can induct engineering bacteria to accumulate red pigment. After HPLC and absorption spectral analysis, the pigment synthesized in the engineering bacteria is the lycopene, the biomass (dry weight) of the engineering bacteria is 2.36gDCW / L, and the lycopene content is 1.52mg / gDCW. The method which lays the preliminary foundation for realizing industrial production of lycopene has potential application value in the production practice.

Description

A kind of preparation method of lycopene technical field The invention belongs to the field of bioengineering, in particular to a method for biosynthesizing lycopene. Background technique Lycopene is an important carotenoid with a molecular formula of C40H56 and a molecular weight of 536.85. Recent studies have shown that the antioxidant properties of lycopene are the strongest among natural carotenoids, and its unique physiological functions have attracted more and more attention: it can efficiently quench singlet oxygen and scavenge peroxide free radicals; through Affect the activity of human cells and the absorption and circulation of nutrients, control the growth and metabolism of cells; as a low-cholesterol agent, inhibit cholesterol biosynthesis enzymes, regulate cholesterol metabolism, and prevent and treat cardiovascular diseases. Therefore, lycopene has important applications in the fields of food, cosmetics and medicine. At present, lycopene can be obtained th...

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Problems solved by technology

Studies have shown that although the crt gene family in the mutant strain TC72 is complete, the carotenoid synthesis pathway stays at phytoene due to the insertion of Tn5, and the expression of crtI and subsequent genes is interrupted (as shown in Figure 1 Show)

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