Chemical and biological agents for the control of molluscs

Inactive Publication Date: 2010-10-21
POINT FINANCIAL
View PDF2 Cites 29 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0023]In a related aspect, the invention is directed to the use of an inert material for increasing the efficacy of one or more substances for controlling one or more molluscs, particularly a class of molluscs selected from the group consisting of Bivalvia, particularly, mussels (e.g., Dreissana sp.) and/or Gastropoda, particularly, snails, which includes but is not limited to aquatic snails (e.g., Biomphalaria sp.) and garden snails, including but not limited to brown garden snails, white garden snails (e.g., Cantareus sp., Cornu sp., Theba sp.), and/or slugs, including but not limited to gray garden slug (e.g., Deroceras sp.), the banded or three-band slug (e.g., Lehmannia sp.), the tawny slug (e.g., Limacus sp.), and the greenhouse slug (e.g., Milax sp.), in a location where control is desired. The location may be a liquid (e.g., a body of water or paint) or solid surface, such as plastic, concrete, wood, fiberglass, pipes made of iron and polyvinyl chloride, surfaces covered with coating materials and/or paints. In p

Problems solved by technology

The ability of the mussels to quickly colonize new areas, rapidly achieve high densities and attach to any hard substratum (e.g., rocks, logs, aquatic plants, shells of native mussels, and exoskeletons of crayfish, plastic, concrete, wood, fiberglass, pipes made of iron and polyvinyl chloride and surfaces covered with conventional paints) make them to cause serious adverse consequences.
These consequences include damages of water-dependent infrastructure, increased millions of dollars in the operating expense and significant damage of the ecological systems [O'Neill, C. R., Jr.
However, it is unlikely that mussel population will be controlled by natural predation, especially in man-made structures such as pipes or pumping plants.
However, mussels can withstand this treatment for several days by closing their shells and chlorine can be only used in pipes or ducts that contain pressure sensing or other equipment due to environmen

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Chemical and biological agents for the control of molluscs
  • Chemical and biological agents for the control of molluscs
  • Chemical and biological agents for the control of molluscs

Examples

Experimental program
Comparison scheme
Effect test

example 1

Molluscicide Studies

Materials and Methods

[0083]1. Allow quagga mussels to acclimate in the small Petri dishes for 24 hrs.[0084]Pour the mussels into a Petri dish to determine if the mussels are alive or not. Toss out the dead and empty mussels.[0085]Count out 10 live / healthy mussels.[0086]Put 10 live / healthy mussels in each small Petri dish with hard water.[0087]Keep a separate plate of mussels. These are the “extra mussels” that will be used to replace dead or empty mussels after 24 hrs in the other experimental plates.[0088]Aeration is not needed due to the low volume of water (DO is high).

[0089]2. Day of mussel treatment:[0090]Check the mussels (Use the rubber policemen at all times when checking the mussels. Only use the tweezers to remove dead mussels).[0091]Count to make sure there are 10 live / healthy mussels per small Petri dish.[0092]Get the sample(s) ready.[0093]Dilute appropriately with hard water in a 50 ml falcon tube for each sample. Vortex to mix prior to dosing.[0094]...

example 2

Erwinia Extracts

[0122]Erwinia carotovora is grown on LB broth (per liter: 10 g tryptone, 5 g yeast extract, 10 g NaCl, pH=7.5). Inoculum is grown by streaking a TSA (tryptic soy agar) plate from a glycerol stock. Purity of the culture is confirmed through visual inspection of colony morphology. Using a sterile 10 μL loop, colonies are collected from the agar surface and resuspended in 50 ml of LB broth in a 250 ml non-baffled Erlenmeyer flask with screw cap. The liquid culture is incubated for 48-72 hours at 200 rpm and 25° C.

[0123]After 72 hr, the whole broth is extracted with ethyl acetate. The organic phase is dried under vacuum. The dried extracted is made a 5.0 mg / mL solution in dimethyl sulfoxide (DMSO). Then, such solution (100 μL) is added into 45 mL hard water. The final concentration of ethyl acetate extracts is 11.1 ppm.

[0124]Data shown in Table 3 indicates that bioactive compounds against the quagga mussels are produced in Erwinia carotovora when grown in the LB media. T...

example 3

Isolation of Molluscicidal Compounds from Pseudomonas

Study A

Fractionation of Compounds

[0125]The following procedure is used for the fractionation of compounds extracted from washed cells of Pseudomonas fluorescens CL-145A:

The cell pellet derived from the 10-L fermentationP. fluorescens CL 145A (ATCC 55799) in FM2 growth medium is suspended in dilution buffer and extracted with Amberlite XAD-7 resin (Asolkar, R. N., Jensen, P. R., Kauffman, C. A., Fenical, W. 2006. Daryamides A-C, Weakly Cytotoxic Polyketides from a Marine-Derived Actinomycete of the Genus Streptomyces strain CNQ-085 J. Nat. Prod. 69:1756-1759; Williams, P. G., Miller, E. D., Asolkar, R. N., Jensen, P. R., Fenical, W. 2007. Arenicolides A-C, 26-Membered Ring Macrolides from the Marine Actinomycete Salinispora arenicola. J. Org. Chem. 72:5025-5034) by shaking the cell suspension with resin at 225 rpm for two hours at room temperature. The resin and cell mass are collected by filtration through cheesecloth and washed ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Fractionaaaaaaaaaa
Fractionaaaaaaaaaa
Timeaaaaaaaaaa
Login to view more

Abstract

Compositions and methods for controlling molluscs, members of the Gastropoda and Bivalvia classes which includes but is not limited to lactones, lactams, carbamates, amides, and/or carboxylic acid containing compounds as active ingredients and/or compounds derived from Pseudomonas and/or Erwinia. Also provided are methods and compositions for increasing the efficacy of chemical and biological control for invasive molluscs in open waters, power plants, and drinking water treatment facilities under coldwater conditions.

Description

PRIORITY CLAIM[0001]This application claims priority from U.S. application Ser. No. 61 / 170,686, filed Apr. 20, 2009, U.S. application Ser. No. 61 / 170,790, filed Apr. 20, 2009 and U.S. application Ser. No. 61 / 170,790, filed Dec. 10, 2010, the contents of which are incorporated herein by reference.FIELD OF THE INVENTION[0002]Compositions and methods for controlling molluscs, such as mussels and / or snails and / or slugs which includes but is not limited to lactones, lactams, carbamates, amides, and / or carboxylic acid containing compounds as active ingredients and / or compounds derived from a microbe (e.g., Pseudomonas and / or Erwinia). Also provided are methods and compositions for increasing the efficacy of chemical and biological control for molluscs, such as mussels and / or snails and / or slugs in open waters, power plants, and drinking water treatment facilities under coldwater conditions or solid surfaces.BACKGROUND OF THE INVENTION[0003]The Zebra mussel, Dreissena polymorpha, was origi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01N63/02C09D5/16C07D307/20C07C59/01C07D309/12C07C233/02C07D207/16C07D211/60C07D223/06A01N43/08A01N43/16A01N37/02A01N37/18A01N43/36A01N43/40A01N43/46A01P9/00A01N63/20A01N63/27
CPCA01N37/18C07D211/16A01N37/46A01N43/08A01N43/36A01N43/76A01N49/00A01N63/02C07C59/42C07C233/06C07C233/09C07D207/04C07D211/06C07D223/04C07D295/18C07D307/33C07D309/30C09D5/1625A01N37/02A01N43/16A61K35/74C07D207/06A01N37/36A01N63/27A01N63/20Y02A90/40A01N63/10A61K35/618
Inventor ASOLKAR, RATNAKARDOW, SARAHANNHUANG, HUAZHANGKOIVUNEN, MARJAMARRONE, PAMELASHU, STEPHANIE
Owner POINT FINANCIAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products