Bacillus subtilis BSn5 with bacteriostasis active to carrot soft rot Erwinia and bacteriostasis protein APn5

A Bacillus subtilis and carrot soft rot technology, applied in the direction of bacteria, bacterial peptides, fungicides, etc., can solve the problems of large-scale damage, rapid spread, and difficult control

Inactive Publication Date: 2008-04-23
HUAZHONG AGRI UNIV
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  • Application Information

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Problems solved by technology

Generally, konjac grows well in the first year of planting, with few diseases; in the second year of planting, a small amount of disease occurs; in the third year of planting, a large number of diseases occur, sometimes a large area is affected, and the disease is more likely to occur in the rainy season, and the incidence rate can reach more than 80%. , in severe cases, it will cause the seedlings to fall in pieces, resulting in no harvest in the whole field
The soft rot pathogen invades from the root of konjac and the wound on the surface of the plant. It has the characteristics of long incubation period, fast transmission and difficult control. At present, there is no effective control method

Method used

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  • Bacillus subtilis BSn5 with bacteriostasis active to carrot soft rot Erwinia and bacteriostasis protein APn5
  • Bacillus subtilis BSn5 with bacteriostasis active to carrot soft rot Erwinia and bacteriostasis protein APn5
  • Bacillus subtilis BSn5 with bacteriostasis active to carrot soft rot Erwinia and bacteriostasis protein APn5

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Embodiment 1

[0030] 1. Isolation, identification and antibacterial activity detection of Bacillus subtilis BSn5 strain

[0031] 1. Isolation and screening of endophytic antagonistic bacteria against konjac soft rot

[0032] Rinse the tubers of Amorphophallus konjac under tap water for 15 minutes to clean the surface dirt. Afterwards, the tuber surface is sterilized, and the method is as follows: soak in 0.15% (m / v) mercury chloride solution for 30 minutes, then soak for 2 minutes with 70% (v / v) alcohol, and finally rinse 3 times with sterilized water to remove residual chemical reagents on the surface . Put the surface-sterilized tubers in sterilized water to peel off the skin, and then cut into about 0.5cm 3 Blot the surface moisture with sterilized absorbent paper, inoculate in Amorphophallus konjac callus induction medium (MS basic medium+3% sucrose+0.7% agar+NAA (naphthalene acetic acid) 0.5mg / L+6-BA (6-benzylaminopurine) 0.5mg / L, add water to 1L, pH 6, sterilize at 121°C for 30min)...

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Abstract

The present invention belongs to the field of agricultural microbiological technology, and discloses Bacillus subtilis BSn5 possessing bacteriostasis activity on carrot soft rot erwinia and the bacteriostatic protein APn5. Bacillus subtilis BSn5 in the preservation number of CCTCC No. M207124 is separated from the callus of bacteria infected konjaku. Bacillus subtilis BSn5 has bacteriostasis activity on carrot soft rot erwinia, and the bacteriostatic protein APn5 has molecular weight of 31.6 kDa.

Description

technical field [0001] The invention belongs to the technical field of microorganism application. Specifically, it relates to the isolation of a strain of Bacillus subtilis that has antibacterial activity against Erwinia carotovora and the purification and identification of its antibacterial protein, and is related to the technical field of microbial pesticides. Background technique [0002] Bacillus subtilis (Bacillus subtilis) is a Gram-positive rod-shaped bacterium with no capsule, perinatal flagella, and can move. It is a non-pathogenic bacterium that widely exists in nature and is also a common plant endophyte , can colonize various organs of plants. Bacillus subtilis can produce a variety of metabolites with antibacterial biological activity. These antibacterial substances are mainly divided into two categories, one is low-molecular-weight antibacterial peptides, including cyclic peptides or cyclic lipopeptides, some are linear peptides, and a small amount is protein...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C07K4/04A01N63/02A01P3/00C12R1/125
Inventor 孙明喻子牛周盈韩冬梅
Owner HUAZHONG AGRI UNIV
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