Anti-CD5 protein monoclonal antibody as well as cell strain, preparation method and application thereof

A monoclonal antibody and protein technology, applied in the field of biomedical engineering, can solve problems such as unclear mechanism and achieve high specificity
CN113105547AActive Publication Date: 2021-07-13FUZHOU MAIXIN BIOTECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
FUZHOU MAIXIN BIOTECH CO LTD
Publication Date
2021-07-13

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Abstract

The invention relates to a monoclonal antibody capable of recognizing a human CD5 antigen, a secretory cell strain, a preparation method of the monoclonal antibody and application in immunodetection. According to the technical scheme, amino acids from the 398th site to the 495th site of the C terminal of the CD5 protein are selected as antigen peptides, codon optimization is carried out, a gene segment suitable for being expressed in escherichia coli BL21 is formed, and the finally obtained recombinant protein comprises a CD5 protein segment and a histidine protein tag. A mouse is immunized by the recombinant protein, and the mouse hybridoma cell strain 11H3 capable of efficiently secreting the anti-CD5 protein monoclonal antibody and the anti-CD5 protein monoclonal antibody secreted by the cell strain are obtained through cell fusion, screening and subcloning. The antibody obtained by the scheme has high specificity and sensitivity, can specifically recognize cells expressing CD5 protein, and is suitable for immunological detection, especially immunohistochemical detection.
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Description

technical field

[0001] The invention relates to the field of biomedical engineering, in particular to an anti-CD5 protein monoclonal antibody and its cell line, preparation method and application. Background technique

[0002] The human CD5 gene is located on chromosome 11q12.2. The CD5 molecule is a 67KD transmembrane glycoprotein expressed in a subclass of T cells and virgin B cells. CD5 is a macrophage scavenger receptor rich in cysteine (SRCR) Domain homology domain receptor family members, these CD5-positive B cells can produce autoantibodies, and can also spread in autoimmune diseases and lymphoid malignancies, such as leukemia, MCL and CD5-positive The DLBCL. Unlike mouse B1 cells that express CD5, human B1 cells do not have CD5 expression. Experiments in chicken egg lysozyme transgenic models in mice and chicken CD5-knockout cells showed that CD5 expression promotes tolerance to self-reactive antigens in vitro, while the absence of CD5 molecules leads to enhanced c...

Claims

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