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Personalized tissue repairing scaffold capable of realizing pulsed sustained release and preparation method thereof

A tissue repair and pulse-type technology, which is applied in the field of personalized tissue repair scaffolds and its preparation, can solve the problems of short release period of sustained-release microspheres, achieve good biocompatibility and degradability, prolong the sustained-release period, Effect of long release period

Active Publication Date: 2015-05-20
RESEARCH INSTITUTE OF TSINGHUA UNIVERSITY IN SHENZHEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the release period of the existing slow-release microspheres is generally short, usually only a few weeks to a few months

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Preparation of sustained-release microspheres with BMP-2 loaded core layer material being PLGA, middle layer material being Eudragit L100-55, and outer layer material being chitosan.

[0036] Take 0.6g of PLGA with an average molecular weight of 100kDa and dissolve it in 6mL of 100mg / mL DCM solution, then add 0.3mL of 2mg of BMP-2 in PBS solution; emulsify the DCM solution with a high-speed disperser at 4500rpm for 1min , Prepare the inner emulsion; add the inner emulsion to 120 mL of an aqueous solution containing 1.2 mL of emulsifier T-80, and emulsify it with a high-speed disperser at 8000 rpm for 1 min to prepare a double emulsion; the double emulsion is at room temperature at 500 rpm Stir mechanically at a rotating speed overnight to volatilize the DCM therein; stand still, centrifuge, wash, and freeze-dry to obtain nuclear layer microspheres formed by PLGA dispersed with BMP-2.

[0037] Take 60 mg of the above core layer microspheres and disperse them in 6 mL metha...

Embodiment 2

[0044] (1) Preparation of sustained-release microspheres in which the core layer material carrying DOX is PLGA, the middle layer material is Eudragit L100-55, and the outer layer material is chitosan.

[0045] Take 0.6g of PLGA75 / 25 with an average molecular weight of 100kDa and dissolve it in 6mL of 100mg / mL DCM solution, then add 0.3mL of dimethyl sulfoxide (hereinafter referred to as DMSO) solution containing 6mg of DOX; The DCM solution was emulsified for 1 minute at a rotation speed of 4500 rpm to prepare an inner emulsion; the inner emulsion was added to 120 mL of an aqueous solution containing 1.2 mL of emulsifier T-80, and a high-speed disperser was used to emulsify at a rotation speed of 8000 rpm for 1 minute to prepare a double emulsion; The double emulsion was mechanically stirred overnight at a speed of 500 rpm at room temperature to volatilize the DCM in it; standing, centrifuged, washed, and freeze-dried to obtain core layer microspheres formed by DOX-dispersed PLGA....

Embodiment 3

[0053] The steps of this embodiment are basically the same as the above-mentioned embodiment 1, the difference is that after three-dimensional printing and forming the matrix, it further includes: filling the holes of the matrix with a polymer hydrogel, and forming it by a low-temperature freeze-drying method , Forming a personalized tissue repair scaffold with hierarchical structure.

[0054] Specifically: add 2 parts of 3% chitosan acetic acid / sodium acetate aqueous solution to 100 parts of 0.3% collagen-containing acetic acid / sodium acetate aqueous solution, stir and mix, and then add 2 parts of 20% glutaraldehyde to it The aqueous solution is stirred and mixed to make the collagen and glutaraldehyde cross-linked to form a polymer compound hydrogel; the personalized tissue repair scaffold obtained by three-dimensional printing is placed in a PTFE template, and then the polymer compound water The gel is cast into the matrix hole of the personalized tissue repair scaffold until ...

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PUM

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Abstract

The invention provides a personalized tissue repairing scaffold for bone, cartilage, skin injury and other injury repairing fields. The personalized tissue repairing scaffold is molded by means of a three-dimensional printing technology, and comprises a substrate and a plurality of sustained-release microspheres dispersed in the substrate, wherein each sustained-release microsphere is of a three-layer structure, namely a core layer, an intermediate layer and an outer layer, and growth factor and / or drugs are loaded on the core layer and outer layer of each sustained-release microsphere. According to the characteristics of treatment positions, degradable high-molecular compound hydrogel or degradable high-molecular / inorganic hybrid hydrogel can be further poured into holes of the substrate, and molding is achieved by means of a low-temperature freeze-drying method. The invention further provides a method for preparing the personalized tissue repairing scaffold. The personalized tissue repairing scaffold has excellent biocompatibility and degradability by adopting the sustained-release microspheres with three-layer structures, is used for pulsed sustained release of growth factor and / or drugs to achieve the effect of long-time stable sustained release, and is beneficial to prolonging of sustained-release cycle of growth factors and / or drugs.

Description

Technical field [0001] The invention relates to the field of biomedical materials, in particular to a personalized tissue repair scaffold applied in the field of bone, cartilage, skin defect and other trauma repair and a preparation method thereof. Background technique [0002] Bone and cartilage repair materials, skin trauma, burn repair materials, etc. are currently the most clinically demanded biomedical materials. Although these materials have great differences in composition, they all need to meet the structural characteristics of porous scaffolds in structure. The ideal tissue repair scaffold material should have good biocompatibility, three-dimensional connected pore structure and suitable mechanical and degradation properties, which can promote cell adhesion, growth, proliferation, differentiation and metabolism, and can carry and coordinate biologically active factors. The interaction with cells affects the expression of cell surface receptors and cell differentiation, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/48A61L27/42A61L27/54
Inventor 陈昌盛王明波佘振定
Owner RESEARCH INSTITUTE OF TSINGHUA UNIVERSITY IN SHENZHEN
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