Medicine-loaded nanoparticle vector and temperature-sensitive gel compounding system and preparation method thereof

A temperature-sensitive gel and composite system technology, which is applied in the field of nanoparticle carrier temperature-sensitive gel composite system loaded with drugs or genes and its preparation, can solve the problems of long-term safety that need further research, and achieve the extension of drug residence time and slow down the drug retention time. Inflammation, enhances the effect of the repair process

Inactive Publication Date: 2017-03-22
SUZHOU UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

However, the long-term safety of this cell preparati

Method used

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  • Medicine-loaded nanoparticle vector and temperature-sensitive gel compounding system and preparation method thereof
  • Medicine-loaded nanoparticle vector and temperature-sensitive gel compounding system and preparation method thereof
  • Medicine-loaded nanoparticle vector and temperature-sensitive gel compounding system and preparation method thereof

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Embodiment 1

[0036] Polyamide-amine dendrimer (PAMAM) polymer loaded with Kartogenin (KGN) is PAMAM-NH 2 - Preparation of KGN

[0037] Dissolve Kartogenin and triethylamine in 4 mL of dimethylformamide (DMF), add 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC.HCl), and nitrogen protection , reacted at room temperature for 1 h in the dark, then added N-hydroxysuccinimide (NHS), continued the reaction for 12 h under the same conditions, and finally added PAMAM-NH 2 (Molar ratio, the molar ratio of KGN and PAMAM is 25:1) continue to react for 24h. After the reaction was completed, DMF was removed by dialysis with water, purified by Sephadex G25 gel column after ultrafiltration and concentration, and the macromolecular part that flowed out first was collected, concentrated by ultrafiltration again, and freeze-dried to obtain a solid that is PAMAM-NH2 -KGN. figure 1 for synthetic products 1 H NMR characterization. Such as figure 1 As shown, 7.3-8.3ppm is the characteristic...

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Abstract

The invention discloses a medicine-loaded nanoparticle vector and temperature-sensitive gel compounding system and a preparation method thereof. The preparation method includes the following steps that nanoparticle vectors are prepared; medicine molecules are connected with amino groups or hydroxyl groups at the ends of polyamidoamine dendrimers in a covalent bonding mode, and a medicine-loaded polyamidoamine dendrimer polymer is prepared; temperature-sensitive gel is prepared, the temperature-sensitive gel is a PLGA-PEG-PLGA copolymer, PLGA is a lactide-glycolide copolymer, and PEG is a polyethylene glycol copolymer; a copolymer solution is prepared by putting the PLGA-PEG-PLGA copolymer into a solvent; and under the condition of stirring, the medicine-loaded polyamidoamine dendrimer polymer is added into the copolymer solution, stirring is continued, and the medicine molecule-loaded nanoparticle vector and temperature-sensitive gel compounding system is obtained. The medicine-loaded nanoparticle vector and temperature-sensitive gel compounding system has a dual slow release function, and can stimulate synovial membrane mesenchymal cells to develop into cartilage cells, relieve the inflammation of a cartilage injured part, and promote the injury repair process of articular cartilages.

Description

technical field [0001] The invention belongs to the technical field of medicine, and more specifically relates to a nanoparticle carrier temperature-sensitive gel composite system loaded with drugs or genes and a preparation method thereof. Background technique [0002] Knee osteoarthritis (OA) is the most common chronic osteoarthritis characterized by the destruction of articular cartilage and bone hyperplasia, and is one of the main causes of joint pain and dysfunction in the elderly. According to the statistics of the World Health Organization, the incidence rate of OA is 50% among people over 50 years old, and 80% among people over 55 years old. The No. 2 killer after cardiovascular disease. The pain and dysfunction caused by OA will affect the quality of life of patients and bring great economic burden to families and society. Therefore, it is of great significance and social value to develop an effective treatment plan for OA. [0003] The place where the bone conne...

Claims

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Application Information

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IPC IPC(8): A61K47/59A61K47/34A61K9/06C08G63/664
CPCA61K47/34A61K9/06C08G63/664
Inventor 程丽芳胡青陈大为
Owner SUZHOU UNIV
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