The present application relates to a kind of methods for calculating
cell picture definition value based on saturation value, provide a kind of methods for calculating
cell picture definition value based on saturation value, comprising the following steps: (1) the color conversion of
cell picture obtained by shooting is carried out, and cell picture is converted from RGB (red green blue) channel to HSV (color, saturation, brightness);(2) separate out saturation channel picture S, remove part of
noise by
Gaussian filtering (or mean filtering), obtain picture S1;(3) select suitable size kernel to picture S1 and carry out
Laplace transform and calculate absolute value, find out the position of change in picture, obtain picture S2;(4) set appropriate threshold, carry out
thresholding processing to picture S2,
record saturation value, find out corresponding
bright spot position;(5) the
bright spot position of multiple cell pictures is counted, and the saturation value of corresponding picture S2 is recorded.This
algorithm is simple, effective, combined with
Gaussian filtering (or mean filtering),
Laplace transform,
decision tree, morphological operation etc., so that the result of judging cell picture definition value is more accurate.