The invention relates to a reflective non-labeled super-resolution optical microscopic
system and method. The reflective non-labeled super-resolution optical microscopic
system comprises a large-numerical-aperture conical super-resolution focusing module, a super-resolution focusing
light field illumination and collection module, a sample and mechanical scanning module, a super-resolution imaging module and a wide-field
microscopic imaging module, the characteristics of super-resolution focal spot, super-long focal depth, high radial
spatial frequency, narrow frequency
passband and the like of a large-numerical-aperture conical super-resolution
light beam are utilized, projection of a super-resolution
light field on front focal planes of two objective lenses is realized by adopting a 4f
system, and a marking-free reflective far-field super-resolution optical microscopic system and method are realized by combining a
confocal working principle; non-marked far-field super-resolution two-dimensional
microscopic imaging is realized through two-dimensional scanning; and performing non-
label far-field super-resolution two-dimensional
microscopic imaging on object planes at different axial positions to obtain super-resolution two-dimensional microscopic imaging of different cross sections of the sample, thereby realizing three-dimensional
chromatography. According to the invention, non-
label, super-resolution and
tomography of the sample can be realized.