Rapid Methods for the Extraction of Nucleic Acids from Biological Samples

US20160333339A1Active Publication Date: 2016-11-17LONGHORN VACCINES & DIAGNOSTICS LLC
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Patent Information

Authority / Receiving Office
US · United States
Current Assignee / Owner
LONGHORN VACCINES & DIAGNOSTICS LLC
Publication Date
2016-11-17

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Abstract

The invention is directed to compositions and methods for rapidly and efficiently extracting nucleic acids and / or targeted nucleic acids sequences from biological samples. The methods of the invention comprise combining the sample with a buffer and magnetic silicon beads and concentrating the beads with a magnet or other electrical field. Liquid may be removed, or not, and an alkaline buffer is added followed by magnetic carboxy beads in a binding buffer so that nucleic acids transfer to the carboxy beads, which can be easily and quickly isolated once again with a magnet. Total nucleic acid extraction is greatly enhanced. Extracted nucleic acids can be analyzed, for example, by PCR wherein the nucleic acids can be identified and characterized. Carboxy beads may also contain a ligand so as to target specific nucleic acid sequences. The invention is also directed to kits comprising the tools and compositions for performing the methods of the invention.
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Description

REFERENCE TO RELATED APPLICATIONS

[0001] This application claims priority to U.S. Provisional Application No. 62 / 232,666 filed Sep. 25, 2015, and U.S. Provisional Application No. 62 / 161,527 filed May 14, 2015, the entirety of each of which is specifically incorporated herein.FIELD OF THE INVENTION

[0002] The invention is directed to rapid methods for the extraction of total and / or targeted nucleic acids from a biological sample and, in particular, to tools, methods and compositions containing components that facilitate concentration and isolation / extraction of nucleic acids from samples.BACKGROUND OF THE INVENTION

[0003] The ability to maintain the integrity of nucleic acids in a biological sample (and in particular, those contained in diagnostic samples obtained from human patients), whether the specimen is taken in a remote field location, a doctor's office or in a laboratory, often determines whether the nucleic acids can be successfully analyzed. Typically, nucleic acids in a biologic...

Claims

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