Six-color real-time fluorescence quantitative PCR (Polymerase Chain Reaction) analyzer
A real-time fluorescence quantitative and analyzer technology, which is applied in the field of PCR analyzers, can solve the problems of inter-interference, weak fluorescent signal, and difficult accurate detection of weak fluorescence, and achieve the effect of reducing the power consumption of the whole machine and low power consumption
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[0022] see figure 2, the applicant designed a six-channel real-time fluorescent quantitative PCR analyzer, in which the six-color optical assembly consists of a light source excitation part A and a photodetector part C. The light source excitation part A generates a light source that excites the fluorescent groups in the sample system to emit light, and the photodetector part C is used to detect the fluorescence emitted by the excited fluorescent groups in the sample system. Based on the principle of fluorescence detection, the two parts of the light source excitation part A and the photodetector part C cannot be in the opposite position, generally at a certain angle, and the optimal angle is 90°, which ensures that the excitation radiation entering through the wall 31 Exit through wall 32 is minimal. At the same time, the 90° angle enables the maximum amount of outgoing emitted light, such as fluorescent light, to be collected through the wall 32 .
[0023] image 3 is a ...
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