Six-color real-time fluorescence quantitative PCR (Polymerase Chain Reaction) analyzer

A real-time fluorescence quantitative and analyzer technology, which is applied in the field of PCR analyzers, can solve the problems of inter-interference, weak fluorescent signal, and difficult accurate detection of weak fluorescence, and achieve the effect of reducing the power consumption of the whole machine and low power consumption

Active Publication Date: 2015-01-07
BEIJING GENOME BIOTECH
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Problems solved by technology

However, the fluorescence signal emitted by the fluorophore in the current PCR reaction system is very weak, and it is difficult to accurately detect the weak fluorescence with the above optical components
[0005] In the prior art, the light sources 100A-100D are green, green, blue and green in color respectively. In actual use, there will be problems such as inter-channel interference, that is, it is difficult to f

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  • Six-color real-time fluorescence quantitative PCR (Polymerase Chain Reaction) analyzer
  • Six-color real-time fluorescence quantitative PCR (Polymerase Chain Reaction) analyzer
  • Six-color real-time fluorescence quantitative PCR (Polymerase Chain Reaction) analyzer

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Embodiment Construction

[0022] see figure 2, the applicant designed a six-channel real-time fluorescent quantitative PCR analyzer, in which the six-color optical assembly consists of a light source excitation part A and a photodetector part C. The light source excitation part A generates a light source that excites the fluorescent groups in the sample system to emit light, and the photodetector part C is used to detect the fluorescence emitted by the excited fluorescent groups in the sample system. Based on the principle of fluorescence detection, the two parts of the light source excitation part A and the photodetector part C cannot be in the opposite position, generally at a certain angle, and the optimal angle is 90°, which ensures that the excitation radiation entering through the wall 31 Exit through wall 32 is minimal. At the same time, the 90° angle enables the maximum amount of outgoing emitted light, such as fluorescent light, to be collected through the wall 32 .

[0023] image 3 is a ...

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Abstract

The invention relates to a six-color real-time fluorescence quantitative PCR (Polymerase Chain Reaction) analyzer. The six-color real-time fluorescence quantitative PCR analyzer comprises a light source excitation part (A) and a light detector part (C), and is characterized in that the light source excitation part (A) is provided with six independent light source excitation passages, the light detector part (C) is provided with six independent detection passages, and a certain angle is formed between the light source excitation part (A) and the light detector part (C). A light path system adopts a fixed light path, light filters are superposed to realize the selection of wavelength coverage, and the wavelength coverage of each passage can be changed by changing the wavelength of the light filters so as to realize different wavelengths.

Description

technical field [0001] The invention relates to a PCR analyzer, more specifically, to a six-color real-time fluorescent quantitative PCR analyzer. Background technique [0002] Nucleic acid amplification reactions are critical for many research, medical, and industrial applications. These reactions are used in clinical and biological research, detection and monitoring of infectious diseases, detection of mutations, detection of cancer markers, environmental monitoring, genetic identification, detection of pathogens in biodefense applications, and more. In particular, the polymerase chain reaction (PCR) has been used in all of these areas, including applications to virus and bacteria detection, viral load monitoring, detection of rare and / or difficult-to-culture pathogens, rapid detection of bioterror threats, cancer Testing for minimal residual disease in patients, food pathogen testing, blood supply screening, and more. With regard to PCR, the main reasons for its widespr...

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Application Information

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IPC IPC(8): G01N21/64G01N21/01
Inventor 陈启跃陈剑飞张洪刚朱福来孙宏伟苑红丽
Owner BEIJING GENOME BIOTECH
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