Sample preparation apparatus

A technology for sample preparation and sample materials, which is applied in the field of sample preparation devices and can solve problems such as limited quantities

Inactive Publication Date: 2010-01-27
SMITHS DETECTION WATFORD LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, difficulties in sample preparation limit t

Method used

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Embodiment Construction

[0017] First refer to figure 1 and Figure 3, which show the sample preparation device for bringing solid or semi-solid sample material into a form suitable for nucleic acid analysis. The sample preparation device has a cylindrical housing 1 with a height of about 200 mm and a diameter of about 120 mm. When in use, the sample preparation device is directly installed on the PCR amplification analysis machine in the vertical direction through the connecting piece 1' located at the lower end of the sample preparation device, and the PCR amplification analysis machine is generally indicated by numeral 2. PCR machine 2 has electric motor 2 ', and this electric motor 2 ' is connected with driving shaft 3 (referring to Figure 3A and Figure 3B ) connection, the drive shaft 3 is located at the center of the sample preparation device and extends along the axial direction of the sample preparation device. PCR machine 2 provides power to the sample preparation device. The sample mate...

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Abstract

The invention provides an apparatus for preparing a biological sample for PCR analysis has an inlet (4) for receiving the sample (15) and a sieve (13) through which the sample is forced by screwing down an inlet cap (16). A lysis solution between two rupturable seals (11 and 12) is effective to disrupt the cells of the sample and release nucleic acid. The apparatus is mounted releasably on a PCR machine (2) having a motor (2') releasably coupled with a drive mechanism (3, 36,40) in the apparatus to effect a rotary and a vertical up and down movement of its components (30, 31, 32, 42). The apparatus has a transparent cuvette (5) and a needle (71) extending to the lower end of the cuvette, by which the prepared sample is dispensed to the cuvette for PCR analysis.

Description

technical field [0001] The invention relates to a sample preparation device having an inlet piece through which liquid and semi-solid sample material can be introduced into said sample preparation device, said inlet piece having a Grooves for sample material. [0002] More particularly but not exclusively, the present invention relates to a device for preparing a sample containing nucleic acid such as DNA or RNA suitable for use in PCR (Polymerase Chain Reaction) techniques. Format for amplification, detection and analysis. Background technique [0003] Traditional techniques for preparing samples for PCR analysis include manually handling the sample and transferring the sample between containers containing different processing solutions. These traditional techniques require skilled workers and are difficult to implement in the wild. For example, in terms of medical, veterinary or agricultural applications, in many cases it is necessary to perform rapid DNA analysis on sa...

Claims

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Application Information

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IPC IPC(8): B01L3/00G01N1/28
CPCG01N1/28B01L3/5082B01L2300/046B01L2400/0683B01L2300/0681B01L3/502B01L2300/087G01N2035/1086
Inventor M·R·费尔斯T·D·福特P·S·哈丁G·S·霍华德B·博伊斯J·W·查杰卡D·J·格林J·莱温顿C·沃尔普C·菲施特J·贝特利C·米尔斯W·R·马韦尔
Owner SMITHS DETECTION WATFORD LTD
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