Sample preparation apparatus

A technology for sample preparation and sample materials, which is applied in the field of sample preparation devices and can solve problems such as limited quantities
CN101636230AInactive Publication Date: 2010-01-27SMITHS DETECTION WATFORD LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SMITHS DETECTION WATFORD LTD
Publication Date
2010-01-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides an apparatus for preparing a biological sample for PCR analysis has an inlet (4) for receiving the sample (15) and a sieve (13) through which the sample is forced by screwing down an inlet cap (16). A lysis solution between two rupturable seals (11 and 12) is effective to disrupt the cells of the sample and release nucleic acid. The apparatus is mounted releasably on a PCR machine (2) having a motor (2') releasably coupled with a drive mechanism (3, 36,40) in the apparatus to effect a rotary and a vertical up and down movement of its components (30, 31, 32, 42). The apparatus has a transparent cuvette (5) and a needle (71) extending to the lower end of the cuvette, by which the prepared sample is dispensed to the cuvette for PCR analysis.
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Description

technical field

[0001] The invention relates to a sample preparation device having an inlet piece through which liquid and semi-solid sample material can be introduced into said sample preparation device, said inlet piece having a Grooves for sample material.

[0002] More particularly but not exclusively, the present invention relates to a device for preparing a sample containing nucleic acid such as DNA or RNA suitable for use in PCR (Polymerase Chain Reaction) techniques. Format for amplification, detection and analysis. Background technique

[0003] Traditional techniques for preparing samples for PCR analysis include manually handling the sample and transferring the sample between containers containing different processing solutions. These traditional techniques require skilled workers and are difficult to implement in the wild. For example, in terms of medical, veterinary or agricultural applications, in many cases it is necessary to perform rapid DNA analysis on sa...

Claims

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