Preparation method and application of GR/WS2-AuNPs-WS2 compound molecular imprinting sensor

A ws2-aunps-ws2, molecular imprinting technology, applied in the intersection of chemistry and materials, to achieve the effect of simple preparation process, mild conditions, high-sensitivity detection and separation

Active Publication Date: 2015-08-12
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the preparation of GR / WS 2 -AuNPs-WS 2 There are no domestic and foreign literatures and related patent reports on the application of composite materials to the detection of L-phenylalanine by molecular imprinting

Method used

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  • Preparation method and application of GR/WS2-AuNPs-WS2 compound molecular imprinting sensor

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Experimental program
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Embodiment 1

[0030] Embodiment 1: a kind of GR / WS 2 -AuNPs-WS 2 The preparation method of composite molecularly imprinted sensor, such as figure 1 shown, including the following steps:

[0031] (1) Take 1g of sodium tungstate and 1.2g of thioacetamide, add 30ml of secondary water and mix evenly, stir with a magnetic stirrer for 2h, then transfer to a 50mL stainless steel reaction kettle, react at 180°C for 36h, take it out and cool it down naturally Centrifuge at room temperature at 5000r / min, wash with distilled water and absolute ethanol three times in sequence, and dry at 60°C for 12 hours to prepare nanosheet transition metal tungsten disulfide;

[0032] (2) get the tungsten disulfide prepared by 0.1g step (1), 0.2mL 10mg / ml chloroauric acid, 0.5g sodium borohydride, be dissolved in secondary water, add 3mL mass fraction in fume hood and be 25% ammoniacal liquor, Ultrasonic oscillation for 30s, heating in a water bath at 60°C for 3.5 hours, cooling to room temperature, standing stil...

Embodiment 2

[0036] Embodiment 2: a kind of GR / WS 2 -AuNPs-WS 2 A method for preparing a composite molecularly imprinted sensor, comprising the following steps:

[0037] (1) Take 1.2g sodium tungstate and 1.5g thioacetamide, add 30ml of secondary water and mix evenly, stir with a magnetic stirrer for 2h, then transfer to a 50mL stainless steel reaction kettle, react at 200°C for 24h, take it out and cool naturally to room temperature, centrifuged at 5000r / min, washed with distilled water and absolute ethanol four times in sequence, and dried at 60°C for 12 hours to prepare nanosheet transition metal tungsten disulfide;

[0038] (2) Take 0.2g tungsten disulfide, 0.3mL 10mg / ml chloroauric acid, 0.5g sodium borohydride, dissolve in secondary water, add 3mL ammonia water with a mass fraction of 27% in the fume hood, ultrasonically oscillate for 50s, 60℃ Heated in a water bath for 3.5 hours under the same conditions, cooled to room temperature, stood still, filtered with suction, took the fil...

Embodiment 3

[0043] A GR / WS 2 -AuNPs-WS 2 A method for preparing a composite molecularly imprinted sensor, comprising the following steps:

[0044] (1) Take 1.5g sodium tungstate and 2.0g thioacetamide, add 30ml of secondary water and mix evenly, stir with a magnetic stirrer for 2h, then transfer to a 50mL stainless steel reaction kettle, react at 200°C for 36h, take it out and cool naturally to room temperature, centrifuged at 5000r / min, washed with distilled water and absolute ethanol for 5 times, and dried at 65°C for 12 hours to prepare nanosheet transition metal tungsten disulfide;

[0045] (2) Take 0.1g tungsten disulfide, 0.2mL 10mg / ml chloroauric acid, 0.6g sodium borohydride, dissolve in secondary water, add 4mL ammonia water with a mass fraction of 28% in the fume hood, ultrasonically oscillate for 30s, 60°C Heated in a water bath under the conditions for 3.5 hours, cooled to room temperature, stood still, filtered with suction, took the filter cake, and dried it in vacuum at -...

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Abstract

The invention discloses a preparation method of a GR / WS2-AuNPs-WS2 compound molecular imprinting sensor. The preparation method includes the steps: 1) preparing tungsten sulfide which is nanosheet transition metal; 2) preparing an AuNPs / WS2 compound; 3) preparing a GR / WS2-AuNPs-WS2 compound; 3) preparing a GR / WS2-AuNPs-WS2 compound modified electrode; 4) taking the GR / WS2-AuNPs-WS2 compound as a substrate of the modified electrode, L-phenylalanine as a template molecule, 2-ethyl-4-methylimidazole as a crosslinking agent and methacrylic acid as a functional monomer to prepare an L-phenylalanine molecular imprinting sensor. The invention further provides an application of the molecular imprinting sensor in detection and separation of L-phenylalanine. L-phenylalanine detection sensitivity of the sensor is greatly improved owing to the GR / WS2-AuNPs-WS2 compound, and the prepared L-phenylalanine molecular imprinting sensor is high in L-phenylalanine sensitivity and selectivity by taking advantages of molecular imprinting.

Description

technical field [0001] The invention relates to the interdisciplinary field of chemistry and materials, in particular to a GR / WS 2 -AuNPs-WS 2 Preparation method and application of composite molecular imprinted sensor. Background technique [0002] Phenylalanine is one of the essential amino acids for the human body. Only the L-type has physiological effects in the human body. Therefore, it is of great significance to detect and resolve the content of L-phenylalanine in the synthesized DL-phenylalanine. important meaning. At present, the commonly used methods for detecting phenylalanine include fluorescence method, high performance liquid chromatography, gas chromatography-mass spectrometry, etc. The above methods are fast and efficient, but at the same time, they have high cost, complicated operation, and cannot identify the two enantiomers. and other shortcomings. The electrochemical detection method has simple equipment and convenient operation, especially the molecul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N31/10G01N27/30
Inventor 王宗花卢冰夏建飞杨敏张菲菲
Owner QINGDAO UNIV
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