Method for mass production of seedling of Jatropha curcas L.
A tissue culture, large-scale technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., to achieve the effect of good application prospects, short cycle and low cost
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[0012] See the following examples: take the stem tip of the tung tree, wash it with clean water, rinse it under running water for 10 minutes, then transfer it to a clean workbench, blot the water, sterilize it with 70% alcohol for 30 seconds, and then transfer it to 0.1% alcohol sterilized in HgCl solution for 6 minutes, rinsed with sterile distilled water for 5 times, and inoculated the shoot tips into the following medium with 30% cornstarch by weight: MS+6-BA0.5mg / L+NAA 2mg / L+sucrose 30g / L, solidified with 4% agar, cultured under light, pH adjusted to 6, temperature 25±1°C, light intensity 1500LX, light time 12 hours a day. About one week after inoculation, the explants can be seen to expand obviously, forming a callus with a diameter of about 1 cm. After the callus was cut, it was transferred to the same medium for subculture. The seedlings were produced from the tissue-cultured shoots of Tunga tungii multi-cycle multiplied in large quantities, and the medium for rooting...
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