Method for mass production of seedling of Jatropha curcas L.
A tissue culture, large-scale technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., to achieve the effect of good application prospects, short cycle and low cost
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[0012] See the following example: Take the stalk tip of Tung tree, wash it with clean water, rinse it under running water for 10 minutes, then turn it to a clean workbench to absorb the water, sterilize it with 70% alcohol for 30 seconds, and then transfer it to 0.1% Sterilized in HgCl 2 solution for 6 min, rinsed with sterile distilled water 5 times, and inoculated the stem tip into the following medium with 30% by weight corn starch: MS+6-BA0.5mg / L+NAA 2mg / L+sucrose 30g / L, solidified with 4% agar, cultivated under light, adjusted to pH 6, temperature 25±1°C, light intensity 1500LX, light time 12 hours per day. Approximately swelling can be seen about one week after the inoculation of the explants, forming a callus with a diameter of about 1 cm. The callus was cut and transferred to the same medium for subculture. Seedlings are produced by tissue culture buds of T. japonicus with multi-cycle proliferation. The medium for rooting is IBA 0.3mg / L+MS, and rooting is carried out afte...
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