Method and apparatus for magnetically separating selected particles, particularly biological cells
a technology of selected particles and magnetic separation, which is applied in the direction of separation processes, instruments, other chemical processes, etc., can solve the problems of reducing the effectiveness of the technique for research or clinical purposes, and achieve the effect of less damage to the membran
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[0021] The apparatus illustrated in FIG. 1 is particularly useful for magnetically separating certain types of target cells, such as lymphocytes, red blood cells, and / or macrophages, from a blood sample.
[0022] The illustrated apparatus includes a sample container 10 to contain the blood sample. Before or after the blood sample is introduced into container 10, it is mixed with magnetic particles, preferably the commercially-available magnetic microbeads, having a selective affinity to magnetically stain or label the target cells in the blood sample within container 10.
[0023] The apparatus further includes another container 11 which serves as a supply of a buffer liquid to be used in the magnetic separation process. The buffer liquid in container 11 may be any of the commercially-available buffer liquids, such as normal saline solution, PBS, and the like.
[0024] The apparatus illustrated in FIG. 1 further includes a feed tube 12 for feeding the buffer liquid from the buffer container 1...
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