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Method for producing single-cell protein feed by means of fermentation of sweet potato residues

A technology for protein feed and sweet potato residue is applied in the field of preparation of single-cell protein feed, which can solve the problems of heavy environmental pollution and difficult development and utilization of sweet potato residue, and achieves the effects of broad market prospects, good growth and reproduction conditions, and increased content.

Inactive Publication Date: 2013-09-04
JISHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the technical problems of difficult development and utilization of a large amount of sweet potato dregs and heavy environmental pollution, the inventor finally invented a method for producing single-cell protein feed by fermenting sweet potato dregs through a large number of experiments

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] (1) Activation and expansion of strains. Wort juice, agar and distilled water are made into a test tube slant culture medium, inserted into Geotrichum candidum under aseptic conditions, cultivated until a white fluffy or powdery film grows on the slant, then sweet potato slices, glucose and Distilled water was used to make a culture solution, and under aseptic conditions, an inoculation needle was used to pick up Geotrichum candidum from a slant test tube, put it into a bottle containing the culture solution, and culture it on a shaker at 28°C for 48 hours for use.

[0012] (2) Ingredients and fermentation. Add 30% corn flour, 3% urea, 0.5% pectinase, 0.4% cellulase and a certain amount of sterile water to the fresh sweet potato residue to make the mixture have a moisture content of 60%. After mixing well, insert the liquid strain obtained in (1), the inoculum amount is 5%, ferment at 28°C for 80 hours, dry and sterilize.

[0013] The single-cell protein feed obtained...

Embodiment 2

[0015] (1) Activation and expansion of strains. Wort juice, agar and distilled water are made into a test tube slant culture medium, inserted into Geotrichum candidum under aseptic conditions, cultivated until a white fluffy or powdery film grows on the slant, then sweet potato slices, glucose and Distilled water was used to make a culture solution, and Geotrichum candidum was picked from a slant test tube with an inoculation needle under sterile conditions, inserted into a bottle containing the culture solution, and cultured on a shaking table at 26°C for 70 hours for use.

[0016] (2) Ingredients and fermentation. Add 38% corn flour, 4.5% urea, 0.4% pectinase, 0.3% cellulase and a certain amount of sterile water to the fresh sweet potato residue to make the mixture have a moisture content of 58%. After mixing well, add the liquid strain obtained in (1), the inoculum amount is 4%, ferment at 27°C for 110 hours, dry and sterilize.

[0017] The single-cell protein feed obtain...

Embodiment 3

[0019] (1) Activation and expansion of strains. Wort juice, agar and distilled water are made into a test tube slant culture medium, inserted into Geotrichum candidum under aseptic conditions, cultivated until a white fluffy or powdery film grows on the slant, then sweet potato slices, glucose and Distilled water was used to make a culture solution, and under aseptic conditions, an inoculation needle was used to pick up Geotrichum candidum from a slant test tube, put it into a bottle containing the culture solution, and culture it on a shaker at 29°C for 28 hours for later use.

[0020] (2) Ingredients and fermentation. Add 26% cornflour, 2.5% urea, 0.7% pectinase, 0.5% cellulase and a certain amount of sterile water to the fresh sweet potato residue, so that the water content of the mixture is 63%. After mixing well, add the liquid strain obtained in (1), the inoculum amount is 7%, ferment at 29°C for 40 hours, dry and sterilize.

[0021] The single-cell protein feed obtain...

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Abstract

The invention discloses a method for producing single-cell protein feed by means of fermentation of sweet potato residues. The method comprises the following steps. (1) Activation and amplification culture of strains. Geotrichum candidum strains are activated by test-tube slant medium which is made from wort, agar and distilled water. Liquid strains are made by using a culture solution which is made from sweet potato slices, glucose and distilled water. (2) Material distribution and fermentation. Corn flour, urea, pectinase, cellulase and a certain amount of sterile water are added into fresh sweet potato residues and stirred. The liquid strains are then inoculated. The mixture are fermented for 20-120 h at the temperature of 25-30 DEG C, and dried and sterilized to obtain the single-cell protein feed. The single-cell protein feed has a protein content of 18-28% and is higher in protein content. The single-cell protein feed is nontoxic and harmless to human and animals. The single-cell protein feed can be used as feedstuffs of animals such as pigs, chickens or the like and has a wide market prospect.

Description

technical field [0001] The invention relates to a method for preparing single-cell protein feed, in particular to a method for producing single-cell protein feed by fermenting sweet potato dregs. Background technique [0002] Sweet potato is also called sweet potato, sweet potato, sweet potato, sweet potato, and sweet potato. It is an annual plant of Convolvulaceae, a trailing herb, more than 2 meters long, lying on the ground and slanting. Sweet potato is used as raw material to make sweet potato starch, vermicelli or starch factory produces sweet potato starch, and every 100kg of fresh potato produces about 75kg of potato residue. A large amount of sweet potatoes are used to make sweet potato starch and vermicelli in my country every year, and these workshops and factories have left a large amount of sweet potato dregs. Due to the short processing cycle, strong seasonality and large output of sweet potatoes, the potato residues cannot be effectively utilized and piled up ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00A23K1/14
Inventor 田宏现
Owner JISHOU UNIVERSITY
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