Pichia pastoris for efficiently converting methanol to produce single cell protein and application of pichia pastoris

A single-cell protein and Pichia pastoris technology, which is applied in the field of Pichia pastoris that efficiently converts methanol to produce single-cell protein, can solve the problems of long fermentation time, low methanol conversion efficiency, and low single-pot yield

Active Publication Date: 2013-04-17
义马煤业集团煤生化高科技工程有限公司
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Problems solved by technology

[0008] In view of the above situation, in order to overcome the defects of the prior art, the object of the present invention is to provide a strain of Pichia pastoris that efficiently converts methanol to produce single-cell protein and its application, so as to effectively solve the problem of low methanol conversion efficiency and long fermentation time in the methanol protein fermentation production process. , The problem of low yield per tank

Method used

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Embodiment Construction

[0024] The specific implementation manners of the present invention will be described in detail below in combination with actual conditions.

[0025] The application of a strain of Pichia pastoris that efficiently transforms methanol to produce single-cell protein, the specific steps are as follows:

[0026] (1) A strain of Pichia pastoris that efficiently converts methanol to produce single-cell proteins is named Pichia pastoris ( Pichia pastoris ) The strain of HGD-01 has been preserved in the China Center for Type Culture Collection, and the preservation number is: CCTCC NO: M2012342;

[0027] (2), the method of producing single-cell protein using Pichia pastoris HGD-01 strain:

[0028] one Cultivation of high-grade seeds: Inoculate 0.5mL of Pichia pastoris HGD-01 strain on the slant medium of YPD test tube (test tube size 180mm×20mm) by streaking method, culture at 30°C for 48 hours, and take 2 cultured Rinse all the slant seeds with 10mL sterile water for each tube, i...

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Abstract

The invention relates to pichia pastoris for efficiently converting methanol to produce single cell protein and an application of the pichia pastoris. The classification designation is pichia pastoris HGD-01. The pichia pastoris is preserved in China Center for Type Culture Collection with the preservation number of CCTCC NO: M2012342. As the pichia pastoris strain is used for methanol protein production, the whole fermentation period is 70-84h, the fermentation time is shortened, and the yield of a single fermentation tank is increased to 140kg of dry weight of the mycoprotein per ton of fermentation liquid.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a strain of Pichia pastoris capable of efficiently transforming methanol to produce single-cell protein and its application. Background technique [0002] At present, all countries in the world attach great importance to the development of protein resources and research on their application technologies, and have made great progress. Among them, the fastest industrialization is single-cell protein production, which is considered to be one of the most effective ways to solve the shortage of human protein resources. [0003] Single cell protein (SCP), also known as microbial protein, refers to the total protein extracted from purely cultured microbial cells, which can be used as a supplement for human and animal protein. Single-cell protein can be produced by fermentation of waste liquids from sugar, paper, starch, wood processing, etc., but the output is too small due to the limitati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12N1/00C12R1/84
Inventor 乔国厚王兰甫苟万晓胡元森张寅曹敏樊崇张国杰许宗浩卫红伟田亚鹏朱广有王霞
Owner 义马煤业集团煤生化高科技工程有限公司
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