Pichia pastoris for efficiently converting methanol to produce single cell protein and application of pichia pastoris

Abstract

The invention relates to pichia pastoris for efficiently converting methanol to produce single cell protein and an application of the pichia pastoris. The classification designation is pichia pastoris HGD-01. The pichia pastoris is preserved in China Center for Type Culture Collection with the preservation number of CCTCC NO: M2012342. As the pichia pastoris strain is used for methanol protein production, the whole fermentation period is 70-84h, the fermentation time is shortened, and the yield of a single fermentation tank is increased to 140kg of dry weight of the mycoprotein per ton of fermentation liquid.

Description

technical field

[0001] The invention relates to the field of bioengineering, in particular to a strain of Pichia pastoris capable of efficiently transforming methanol to produce single-cell protein and its application. Background technique

[0002] At present, all countries in the world attach great importance to the development of protein resources and research on their application technologies, and have made great progress. Among them, the fastest industrialization is single-cell protein production, which is considered to be one of the most effective ways to solve the shortage of human protein resources.

[0003] Single cell protein (SCP), also known as microbial protein, refers to the total protein extracted from purely cultured microbial cells, which can be used as a supplement for human and animal protein. Single-cell protein can be produced by fermentation of waste liquids from sugar, paper, starch, wood processing, etc., but the output is too small due to the limitati...