Method for extracting ribonucleic acid and cell wall polysaccharides in single cell protein by using one-step method

A technology for single-cell protein and ribonucleic acid, applied in the field of extracting ribonucleic acid and cell wall polysaccharides in single-cell protein, can solve the problems of low product quality, complicated extraction process, high energy consumption, etc. high rate effect

Inactive Publication Date: 2010-09-15
黄山市迎客松生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the previous extraction processes were limited to the extraction of one product, and a large number of other substances with high added value were discarded. In particular, the previous extraction process was complex and energy-intensive, and a variety of chemical agents that could cause secondary pollution were generally added. Cause low product quality and environmental pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Extraction of ribonucleic acid and cell wall polysaccharides in single-cell proteins

[0017] 10 grams of single-cell protein prepared with brewer's yeast was added with 20% water of its dry weight, mixed well and then slowly frozen to -2°C. Place it in an 80°C ultrasonic-assisted water bath with a power of 140W and a frequency of 42KHz to thaw at 35°C, and repeat the process of freezing and melting three times to break the wall. Then heat up to 95°C, add 12% sodium chloride, keep warm for 120min, cool down rapidly to 4°C, and centrifuge to obtain the supernatant and cell wall polysaccharide precipitate; wash the precipitate with water twice, dry, weigh, and obtain a total of 2.037 g of cell wall polysaccharides.

[0018] The isoelectric point of the above supernatant was controlled with hydrochloric acid, the protein was precipitated at the isoelectric point pH 4.2, and the centrifuged protein was discarded. The obtained supernatant continued to control the...

Embodiment 2

[0019] Example 2 Extraction of ribonucleic acid and cell wall polysaccharides in single-cell proteins

[0020] 10 grams of single-cell protein prepared with brewer's yeast was added with 10% water of its dry weight, mixed well and then slowly frozen to 0°C. Thaw in a 50°C ultrasonic-assisted water bath with a power of 100W and a frequency of 30KHz for 10 minutes, and break the wall once during the freezing and melting process. Heat up to 75°C, add 5% sodium chloride, keep warm for 60min, cool to 0°C, and centrifuge to obtain the supernatant and cell wall polysaccharide precipitate; the precipitate is washed twice with water, dried, and weighed to obtain a total of 1.087 grams of cell wall polysaccharides.

[0021] The above supernatant precipitates protein at the isoelectric point pH 4.2, centrifuged protein is discarded, the obtained supernatant continues to control the isoelectric point, and the isoelectric point pH 2.5 is allowed to stand for 2 hours to precipitate RNA, wa...

Embodiment 3

[0022] Example 3 Extraction of ribonucleic acid and cell wall polysaccharides in single-cell proteins

[0023] 10 grams of single-cell protein prepared with brewer's yeast was added with 30% water of its dry weight, mixed well and then slowly frozen to -3°C. Thaw in a 100°C ultrasonic-assisted water bath with a power of 600W and a frequency of 58KHz for 80 minutes, and repeat the process of freezing and dissolving 5 times. Heat up to 100°C, add 18% sodium chloride, keep warm for 140min, cool to 4°C, and centrifuge to obtain the supernatant and cell wall polysaccharide precipitate; the precipitate is washed twice with water, dried, and weighed to obtain a total of 1.687 grams of cell wall polysaccharides.

[0024] The above supernatant precipitated protein at the isoelectric point pH 4.2, and discarded the centrifuged protein. The obtained supernatant continued to control the isoelectric point, stood still at the isoelectric point pH 2.5 for 2 hours, washed with ethanol, and d...

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Abstract

The invention discloses a method for extracting ribonucleic acid (RNA) and cell wall polysaccharides, in particular to a single cell protein prepared by using brewers' yeast. The method comprises the steps of: adding water with 10-30 percent of dry weight of the single cell protein and then fully mixing, freezing at a low speed, placing into an ultrasonic assisting water bath, repeatedly thawing and breaking cell walls; adding sodium chloride with weight ratio of 5-18 percent, rapidly cooling, and eccentrically separating to obtain supernate and cell wall polysaccharides precipitates; and washing the precipitates by using water and then drying to obtain dry products of the cell wall polysaccharides. The protein is precipitated at an isoelectric point PI4.2 in the supernate, the RNA is participated at an isoelectric point PI2.5, and the dry product of the RNA is obtained by the supernate. Because a mixture of amino acid is separated by adopting methods of repeatedly freezing and thawing for breaking the single cell protein and regulating the isoelectric points, the invention has the characteristics of high separation and extraction efficiency, high purity and simple and convenient method.

Description

technical field [0001] The invention relates to a method for extracting ribonucleic acid and cell wall polysaccharide in single-cell protein. Background technique [0002] Single-cell protein is also called microbial protein, which is an artificially cultivated microbial cell. Single-cell protein is not a pure protein, but a cytoplasmic mass composed of a mixture of proteins, fats, carbohydrates, nucleic acids, nitrogenous compounds, vitamins, and inorganic compounds. It is rich in nutrients, especially the single-cell protein produced by fermentation of brewer's yeast, which has a high content of ribonucleic acid and cell wall polysaccharides, and the brewer's yeast used in this method is a recognized non-toxic and harmless widely used in medicine , industrial and food fields with higher biological safety strains. Most of the previous extraction processes were limited to the extraction of one product, and a large number of other substances with high added value were disca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C07H21/02C07H1/08
Inventor 李丰伯杜继龙吴卫东张俊
Owner 黄山市迎客松生物科技有限公司
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