20 results about "Cell wall polysaccharide" patented technology

The present application relates to a kind of green tea cell wall polysaccharide with hypoglycemic activity extraction and purification method and the green tea cell wall polysaccharide obtained, and five different tea polysaccharides are extracted by sequential extraction method using water, CDTA, saturated sodium carbonate and 1M NaOH and 4M NaOH solution, and the best hypoglycemic activity of green tea cell wall polysaccharide is screened by sugar metabolism enzyme activity inhibition, and further purified by gel column chromatography.Compared with prior art, the whole operation process of the present application has relatively mild reaction condition, can greatly retain the activity of green tea cell wall polysaccharide and reduce the loss of polysaccharide component to the maximum, has the advantages of high extraction rate, low energy consumption, small solvent consumption, simple operation and low economic cost, etc., lays a foundation for the study of the physicochemical properties and biological activity of green tea cell wall polysaccharide, and has important significance for the utilization of green tea cell wall polysaccharide in food, medical care and cosmetics and other fields.

The invention discloses application of a flammulina velutipes transcription factor gene AreA in regulation and control of cell wall polysaccharide synthesis and sporocarp taste. Flammulina velutipes as an important edible mushroom is widely welcomed due to rich nutrition, but the edible experience of consumers is seriously influenced and the market potential of the flammulina velutipes is limited due to the problems that the flammulina velutipes is poor in chewiness, easy to plug teeth and the like. The silent strain of the enoki mushroom areA gene is successfully constructed through an RNA interference technology, it is found that the polysaccharide content of cell walls and the toughness of sporocarp can be reduced through reduction of gene expression, then the taste of the sporocarp is improved, and the market competitiveness of the sporocarp is improved. According to the invention, the areA gene in the flammulina velutipes is effectively silenced through the specifically designed siRNA fragment, so that the polysaccharide content of the cell wall and the toughness of the sporocarp are reduced, and a theoretical basis is provided for reducing the toughness of the cell wall of the flammulina velutipes by using a genetic engineering technical means.

The invention discloses a preparation method of a physical adsorption and biodegradation composite mildew removing agent. The preparation method comprises the following steps: preparing modified montmorillonite: modifying sodium montmorillonite by using hexadecyl trimethyl ammonium bromide; culturing a bacillus subtilis fermentation culture: culturing bacillus subtilis CICC20683 by adopting a two-step method of seed culture and fermentation culture, and collecting thalli; culturing a lactic acid bacteria fermentation culture: culturing lactic acid bacteria CICC6078 by adopting a two-step method of seed culture and fermentation culture, and collecting thalli; and composite preparation: uniformly mixing 30-50 parts by weight of modified montmorillonite, 10-20 parts by weight of yeast cell wall polysaccharide, 20-30 parts by weight of bacillus subtilis fermentation culture, 10-20 parts by weight of lactic acid bacteria fermentation culture, 5-10 parts by weight of astragalus polysaccharide and 3-5 parts by weight of vitamin C, carrying out vacuum drying at 40 DEG C until the moisture content is lower than 8%, and crushing to 80 meshes. The preparation method is simple in process and convenient to operate, the used raw materials and equipment are common industrial raw materials and equipment, the production cost is low, and the preparation method has a good market application prospect.

[Problems] To provide plant-base milk with reduced powdery feel and altered taste.[Solution] The present technique provides an enzyme agent for reducing the powdery feel of a plant-base beverage or food or a plant-base beverage or food material, which contains a cell wall polysaccharide degrading enzyme, and a plant-base beverage or food or a plant-base beverage or food material using the enzyme agent for reducing powdery feel. The present technique also provides a method for producing a plant-base beverage or food or a plant-base beverage or food material, and a method for reducing the powdery feel of a plant-base beverage or food or a plant-base beverage or food material, which include a step of allowing a cell wall polysaccharide degrading enzyme to act on a plant-base raw material.

The invention relates to a compound feed additive used in the laying period of breeding hens and a preparation method thereof, and belongs to the technical field of breeding hens breeding and hatching. The invention discloses a compound feed additive. Comprising the following components in parts by weight: 15 to 25 parts of clostridium butyricum, 25 to 35 parts of bacillus subtilis, 12 to 18 parts of bacillus licheniformis, 5 to 15 parts of vitamin B family, 2 to 4 parts of choline dihydrogen citrate, 2 to 5 parts of herba epimedii extract, 0.2 to 0.4 part of nano L-selenomethionine, 4 to 6 parts of bile acid, 5 to 8 parts of yeast cell wall polysaccharide, 2 to 5 parts of organic zinc, 5 to 15 parts of trace elements and 1 to 3 parts of phytase. Through scientific proportioning and synergistic effect of multiple functional components, the feed improves intestinal health of breeding hens, improves reproductive performance, enhances immunity, promotes digestion and absorption of the breeding hens to nutrient substances in the feed, improves feed conversion rate and reduces feed-egg ratio.

The invention discloses phycobiliprotein and / or red algae oligosaccharide extracted based on a biological soluble wall and a preparation method of the phycobiliprotein and / or the red algae oligosaccharide, and belongs to the technical field of biological functional component extraction. The invention provides a preparation method for simultaneously extracting phycobiliprotein and phycobiliprotein of red algae, which is high in efficiency, low in loss rate and suitable for process amplification, specifically, cell wall polysaccharide in the red algae is matched with a compound enzyme in a matching manner, and multiple enzymes are cooperated, so that the contradiction problem between high-temperature dissolution of the cell wall polysaccharide of the red algae and heat sensitivity of phycobiliprotein is effectively solved, and the yield of the phycobiliprotein and the oligosaccharide of the red algae is improved. The wall breaking efficiency is improved, and cell wall breaking is promoted to form porous and macroporous cell wall structures; the outward migration of intracellular substances such as phycobiliprotein is promoted, so that higher extraction efficiency is obtained. According to the preparation method provided by the invention, the extraction amount of phycobiliprotein reaches 224 g / kg after production conversion, and the dissociation loss of phycobiliprotein polymers is effectively reduced. In addition, the phycobiliprotein is efficiently extracted, meanwhile, the red algae oligosaccharide product is obtained, and the process economic benefit and the raw material utilization rate are improved. The preparation method provided by the invention does not need special equipment, is simple and convenient in process, green, environment-friendly, low in cost and beneficial to industrial production.

The invention belongs to the technical field of sweet tea residue recovery, and particularly relates to a recovery process of polysaccharide in sweet tea residues and application of the recovery process. The process for recovering the polysaccharide in the sweet tea residues comprises the following steps: pretreating the sweet tea residues, performing enzymolysis treatment, and performing separation and purification treatment; the pretreatment comprises deep-eutectic solvent and pulsed electric field treatment; the eutectic solvent comprises choline chloride, chlorinated ethanolamine sodium hydrochloride and lactic acid. According to the recovery process of the polysaccharide in the sweet tea residues, provided by the invention, pretreatment comprises deep-eutectic solvent and pulsed electric field treatment, so that the deep-eutectic solvent permeates into cell structures in the sweet tea residues, and the cell structures are swelled; reversible micropores can be formed in cell membranes and cell walls of the strigose hydrangea leaf residues by applying the pulsed electric field, so that the permeability is greatly improved, the deep eutectic solvent can act deeper, then weak bonds of polysaccharide of the cell walls are broken, and the yield of the polysaccharide is further improved (19% or above).

The present application relates to the technical field of lithium metal battery, in particular to a current collector, a preparation method and application thereof, the current collector comprises a substrate, the surface of the substrate is coated with a coating layer, the coating layer comprises strawberry DNA with cell wall polysaccharide attached; the preparation method comprises the following steps: S1, preparing a second mixed solution containing strawberry DNA; S2, preparing a dry mixture from the second mixed solution; S3, weighing the dry mixture, adding Tris buffer solution, adjusting pH with the Tris buffer solution, and configuring into a third mixed solution; S4, centrifugal rotation of the third mixed solution in a centrifuge, and taking supernatant as a coating solution; S5, dropping the coating solution on the surface of the polished substrate, and baking in an oven to coat the substrate surface, obtaining the current collector; the current collector can not only effectively inhibit lithium dendrite growth and greatly reduce the short circuit risk of lithium metal battery, but also significantly improve the stability and coulomb efficiency of the battery, further improving the performance of the battery.

The invention relates to the field of fruit extracts, in particular to a preparation method for obtaining a fruit extract from whole fruits, which comprises the following steps: S1, pulping: pulping cleaned whole fruits to obtain fruit pulp; s2, mixing: adding pectinase and cellulase into the fruit pulp to start enzymolysis, and performing ultrasonic treatment on the mixed pulp to strengthen mass transfer of enzyme molecules and accelerate degradation of cell wall polysaccharides; s3, distilling, namely distilling the slurry subjected to ultrasonic treatment; s4, blanking is conducted, distilled distillate is extracted, and the fruit extract is obtained.The problems that in a traditional mode, the fragrance and fragrance concentration of the fruit extract are not high, and the fragrance is not lasting are solved, and the effect of improving the fragrance, the fragrance concentration and the lasting fragrance of the fruit extract can be achieved.

This application discloses a polysaccharide for the cell wall of Citrus reticulata peel, its preparation method, and its application. The preparation method of the polysaccharide includes: weighing and drying Citrus reticulata peel, pulverizing, and sieving; extracting the Citrus reticulata peel powder by reflux in an organic solvent, washing the filter residue to remove fat, and then removing starch; washing and drying the filter residue after starch removal to obtain Citrus reticulata peel cell wall material; extracting the Citrus reticulata peel cell wall material with an extraction solution, adjusting the pH of the extraction solution to neutral, concentrating, dialyzing, and freeze-drying to obtain the polysaccharide. The Citrus reticulata peel cell wall polysaccharide is prepared by this method. The application of the Citrus reticulata peel cell wall polysaccharide is its use in the preparation of skin care products or pharmaceuticals that have antioxidant properties and promote skin damage repair.

The invention relates to the technical field of Laifeng ginger planting, and specifically provides a Laifeng ginger optimal harvesting time determination method based on texture characteristics and cell wall polysaccharides, and the method comprises the following steps: S1, time node determination: starting harvesting from the 60th day after sowing, then harvesting for one period every 30-60 days, and at least harvesting for four periods; s2, detection: selecting tender ginger of Laifeng ginger harvested at different periods in the step S1, and detecting the brittleness value, the moisture content, the transverse relaxation time of bound water, the total pectin content and the cellulose content; s3, single score conversion: converting the detection values into corresponding single scores according to a preset five-level segmentation scoring rule; and S4, comprehensively judging different brittleness harvesting periods by adopting a weighted scoring function. According to the method, a three-in-one harvesting standard of time nodes, quantitative indexes and visual characteristics is formed, traditional experience dependence is broken, technical support is provided for large-scale and standardized planting, and standardized development of the Laifeng ginger industry is promoted.

The present application provides a kind of cell wall polysaccharide extracted from Pu'er tea and its extraction method and application.The first aspect of the present application provides a kind of method for extracting cell wall polysaccharide from Pu'er tea, first remove water-soluble polysaccharide and macromolecular substances such as protein;Second, a certain amount of amylase is used to further process the non-water-soluble residue to remove starch;Then, ethylenediaminetetraacetic acid combined with alkali solution is used to extract acidic polysaccharide, and it is found through test that the cell wall polysaccharide extracted by the method has immunoregulatory effect, and the activity is higher than that of polysaccharide extracted by traditional method.Therefore, by the method provided by the present application, the cell wall polysaccharide in Pu'er tea is developed and utilized, which helps to improve the industrial added value, broaden the product category, has higher application prospect and potential value.

The invention discloses an oral vaccine for preventing infectious spleen and kidney necrosis of mandarin fish, and belongs to the technical field of aquatic vaccines. The vaccine is composed of an inactivated virus or recombinant antigen protein of an antigen component infectious spleen and kidney necrosis virus (ISKNV), a slow-release carrier sodium alginate-chitosan composite microcapsule, a compound of an immunologic adjuvant-glucan and yeast cell wall polysaccharide, and a mixture of a protective agent sucrose and mannitol. By optimizing an antigen preparation process and screening a biocompatible slow-release carrier and a high-efficiency adjuvant, the technical problems that an existing oral vaccine antigen is easily degraded by gastrointestinal tract digestive juice and the immunogenicity is insufficient are solved, and targeted release and high-efficiency absorption of the antigen after oral administration are realized; the mandarin fish can be obviously induced to generate specific humoral immune and cellular immune response, and the immune relative protection rate reaches 85% or above. The vaccine is convenient to take orally, small in stress to mandarin fish and low in production cost, and can be applied to prevention of infectious spleen and kidney necrosis in mandarin fish culture on a large scale.

The invention discloses an active microorganism feed composite additive and a preparation method, and relates to the technical field of feed processing. The feed additive is prepared from the following raw materials in parts by weight: 2-4 parts of bacillus amyloliquefaciens, 1.5-3 parts of lactobacillus plantarum, 1-2 parts of saccharomyces boulardii, 4-6 parts of xylooligosaccharide, 6-10 parts of yeast cell wall polysaccharide, 10-15 parts of modified starch, 6-9 parts of trehalose, 2-3 parts of tea polyphenol, 3-5 parts of folium cortex eucommiae extract, 2-3 parts of rosemary extract, 2-3 parts of zinc amino acid chelate, 0.5-1 part of selenium amino acid chelate, 1-2 parts of vitamin B complex and 0.5-1 part of vitamin E meanwhile, the formula does not contain antibiotics, artificially synthesized hormones and harmful additives, the probiotics, the plant extract and other raw materials all meet the feed safety standard, the tea polyphenol, the folium cortex eucommiae extract and the rosemary extract replace artificially synthesized antioxidants, oxidative deterioration of the feed can be inhibited, the quality guarantee period of the feed can be prolonged, meanwhile, residues of harmful substances are avoided, and the feed is safe and environmentally friendly. The quality safety of livestock and poultry products is ensured.

The invention belongs to the technical field of biological fermentation, and provides beer yeast vitamin B enriched fermentation liquor and a preparation method thereof. The preparation method comprises the following steps: extracting beer yeast cell wall polysaccharide by adopting an alkaline method, carrying out high-pressure homogeneous nano-fibrosis treatment, endowing negative charges through phosphorylation modification of sodium trimetaphosphate, and constructing a shell layer on the surface of a positive electricity coating beer yeast body formed by epsilon-polylysine through electrostatic deposition; core-shell ion cross-linked immobilized particles are formed by calcium ion cross-linking and used for fermentation, efficient enrichment with the vitamin B1 content being 1-50 mg / L and the vitamin B2 content being 0.5-30 mg / L is achieved, the particle shell thickness is 3-20 microns, the anti-shearing stability is guaranteed, efficient cross-shell mass transfer of substrate oxygen and products is considered, and the yield of the product is increased. The technical problems that structural stability and mass transfer efficiency of traditional immobilized fermentation are difficult to consider at the same time, rheological control of a high-solid-content system is limited, and long-term stability of a shell layer in a dynamic fermentation environment is insufficient are solved, and the method has wide application value.

The invention provides a traditional Chinese medicine extraction and leaching method, and relates to the field of traditional Chinese medicine extraction. Comprising the following steps: S1, crushing medicinal materials, S2, performing enzymolysis at 40-50 DEG C for 20-30 minutes, S3, adding a decoction extracting solution into medicinal material powder, performing decoction at 90-100 DEG C for 20-30 minutes, performing filtration, and collecting decoction liquid and filter residues, filling percolation tanks of a multi-stage countercurrent percolation device with the filter residues respectively, introducing a percolation extracting solution for countercurrent percolation treatment, and collecting percolation liquid and discharged filter residues; s4, adding the decoction extracting solution into the filter residues, decocting at 90-100 DEG C for 20-30 minutes, filtering to obtain decoction and filter residues, and repeating the step S3 on the filter residues; and S5, repeating the steps S3 and S4 for multiple times, and combining the percolation liquid and the decoction liquid to obtain the leachate. Cell wall polysaccharide is degraded through enzymolysis pretreatment, then decoction and multi-stage countercurrent percolation are combined, and the extraction effect is better than that of single percolation; the extraction efficiency of the effective components is improved, the extraction time can be shortened, the use amount of the extracting solution is reduced, and the cost is saved.

The present application belongs to the technical field of microbial detection and analysis, and relates to a kit for detecting bauman acinetobacter and a use method thereof. The kit is an innovative dual-mode (fluorescence / colorimetric) diagnostic system specially designed for rapid detection of bauman acinetobacter, and the core is to construct a "magnetic sandwich" composite composed of a SEQ ID NO. 1 aptamer modified magnetic bead and a boron acid functionalized MOF-on-MOF heterostructure material. Through the high specificity biological recognition of the aptamer to the bacterial surface antigen, the system cooperates with the covalent affinity of the phenylboronic acid group and the bacterial cell wall polysaccharide to form a double locking mechanism, which not only significantly improves the capture efficiency and signal strength, but also effectively overcomes the limitations of a single recognition mode.

The invention relates to a fresh rehmannia root cell wall polysaccharide composition, an extraction method and application, and belongs to the field of plant extraction application, cell wall polysaccharides are gradually extracted from FRR by adopting a sequential extraction method, and three cell wall pectic polysaccharides (WSP, CSP and NSP) and two hemicellulosic polysaccharides (KSH-1 and KSH-2) are successfully obtained. The result shows that the CSP and the NSP show relatively uniform molecular weight distribution. In the aspect of composition, the WSP, the CSP and the NSP consist essentially of Ara and GalA, while the KSH-1 and the KSH-2 contain essentially Ara and Xyl. The FRR-sourced polysaccharide has a good prospect as a functional food or medicine component with antioxidant and immunomodulatory functions.

The invention belongs to the technical field of livestock feed, and discloses a composite boar feed additive and a preparation process thereof. The additive is prepared from tea polyphenol, rosmarinic acid, quercetin, enteric sodium butyrate, yeast cell wall polysaccharide, embedded bacillus subtilis metabolite and a modified starch carrier, the activity of urease and deaminase of posterior intestinal ammonia-producing bacteria is inhibited through multi-component synergism, and nitrogen is regulated and controlled to be converted into mycoprotein. The preparation process comprises the following steps: dissolving and attaching active ingredients, coating enteric-coated pellets, embedding double-layer microcapsules and performing multi-stage mixing. According to the invention, enzyme inhibition functions of tea polyphenol, rosmarinic acid and quercetin are integrated with intestinal barrier strengthening and pH regulation of sodium butyrate, flora regulation of yeast cell wall polysaccharide and targeted bacteriostasis functions of embedded bacillus subtilis metabolites, and accurate release in specific sections of intestinal tracts is realized through enteric coating and microcapsule technologies; on the premise that the protein level of daily ration is not reduced and the reproductive performance is not damaged, the ammonia nitrogen emission is obviously reduced.

This study aims to elucidate the active mechanism responsible for the intestinal barrier protection effect of Bifidobacterium adolescentis. [Solution] An intestinal barrier protectant comprising a polysaccharide having a repeating structure of the cell wall polysaccharide fraction and / or intracytoplasmic fraction of Bifidobacterium adolescentis, rhamnose, glucose, and galactose in a ratio of 4-3:1:2-1, with a fractional molecular weight of 1-30,000 determined by ultrafiltration membrane, or uridine diphosphate-N-acetylmuramic acid-L-alanine-D-glutamic acid-L-lysine as an active ingredient.