41 results about "Cell wall polysaccharide" patented technology

The invention provides an ultrasonic-assisted red pomelo polyphenol extraction method and application. The polyphenol component in the red pomelo is extracted through the following steps: firstly, performing activation pretreatment on red pomelo dry powder by adopting a low-temperature plasma device, and destroying a cell wall structure so as to improve the accessibility of combined polyphenol; then cellulase, pectinase, hemicellulase and beta-glucosidase are added into a biodegradable deep eutectic solvent to synergistically hydrolyze the cell wall polysaccharide, and combined polyphenol is released; then applying pulse type ultrasonic wave to assist extraction under a low-temperature condition, so that polyphenol is fully dissolved out and enters the eutectic solvent; and finally, carrying out solid-liquid separation to obtain the red pomelo polyphenol extract. Compared with a conventional heat extraction method, the method has the advantages that the extraction rate of the polyphenol of the red pomelo is remarkably increased at a lower temperature, the degradation of the polyphenol is effectively avoided, and the content of the total polyphenol and the antioxidant activity are increased; the method is green and environment-friendly, and can be applied to the health industry as an antioxidant.

The present invention relates to the field of feed, and in particular to a feed additive for preventing diarrhea in young ruminants and its application in liquid feed and solid feed. The composition provided by the present invention comprises 5-10wt.% of flavonoids, 5-10wt.% of plant essential oils and 80-90wt.% of yeast cell wall polysaccharides. The composition of the present invention is formulated with flavonoids, plant essential oils and yeast cell wall polysaccharides, and prevents the occurrence of calf diarrhea by regulating the calf's immune system, intestinal mucosal barrier system and intestinal microbial system, improving the calf's body immunity, enhancing the intestinal mucosal barrier function and promoting the balance of intestinal flora, thereby achieving the purpose of promoting the growth and development of calves and healthy cultivation.

The invention belongs to the technical field of microbial fermentation, and particularly discloses a method for degrading anti-nutritional factors of rapeseed dregs through probiotic fermentation and application of the method, and the method comprises the following steps: respectively inoculating aspergillus niger, saccharomyces cerevisiae, bacillus velezensis and lactobacillus paracasei into a liquid culture medium, and culturing to obtain corresponding fermentation seed solutions; inoculating the aspergillus niger fermentation seed solution into rapeseed meal, and performing aerobic solid-state fermentation; then inoculating the bacillus velezensis fermentation seed solution, the saccharomyces cerevisiae fermentation seed solution and the lactobacillus paracasei fermentation seed solution, and carrying out anaerobic solid-state fermentation to obtain a semi-finished product of fermented rapeseed meal; and drying the fermented rapeseed meal semi-finished product to obtain a fermented rapeseed meal finished product. Compared with the prior art, the method has the advantages that a multi-strain step-by-step fermentation method is adopted, antinutritional factors in the rapeseed dregs can be degraded by utilizing a complex enzyme system of microorganisms in the fermentation process, meanwhile, polysaccharide, crude protein and the like of cell walls can be hydrolyzed, and the nutritional value of the rapeseed dregs is increased.

The invention discloses a premix feed additive for laying hens and a preparation method thereof, and the premix feed additive for laying hens is prepared from the following raw materials in parts by weight: 10-20 parts of allicin, 30-50 parts of yeast cell wall polysaccharide, 50-80 parts of kelp powder, 20-40 parts of radix astragali extract, 10-30 parts of humic acid, 5-10 parts of zinc methionine and 2-8 parts of selenium powder. 5-10 parts of a probiotic metabolite and 2-5 parts of a plant essential oil compound. The preparation method of the premix feed additive for laying hens comprises the following preparation processes: S1, preparing allicin; s2, preparing yeast cell wall polysaccharide; s3, preparing kelp powder; s4, preparing a radix astragali extract; s5, preparing humic acid; s6, preparing methionine; s7, preparing selenium powder; and S8, preparing a probiotic metabolite. The additive provided by the invention can realize nutrition balance, meet the nutritional requirements of the laying hens in all stages of growth and egg laying, and improve the feed utilization rate, thereby improving the egg yield of the laying hens and the quality of eggs.

The present application relates to a kind of green tea cell wall polysaccharide with hypoglycemic activity extraction and purification method and the green tea cell wall polysaccharide obtained, and five different tea polysaccharides are extracted by sequential extraction method using water, CDTA, saturated sodium carbonate and 1M NaOH and 4M NaOH solution, and the best hypoglycemic activity of green tea cell wall polysaccharide is screened by sugar metabolism enzyme activity inhibition, and further purified by gel column chromatography.Compared with prior art, the whole operation process of the present application has relatively mild reaction condition, can greatly retain the activity of green tea cell wall polysaccharide and reduce the loss of polysaccharide component to the maximum, has the advantages of high extraction rate, low energy consumption, small solvent consumption, simple operation and low economic cost, etc., lays a foundation for the study of the physicochemical properties and biological activity of green tea cell wall polysaccharide, and has important significance for the utilization of green tea cell wall polysaccharide in food, medical care and cosmetics and other fields.

The invention discloses a pig breeding feed composition based on bacterium-enzyme synergy. The pig breeding feed composition comprises a piglet feed, a growing pig feed and a fattening pig feed, the piglet feed, the growing pig feed and the fattening pig feed at least comprise bacillus coagulans, clostridium butyricum, phytase, amylase, trypsin and NSP enzyme, and the bacillus coagulans can produce lactic acid and bacteriostats to enhance the intestinal barrier function of bred pigs; the clostridium butyricum can produce butyric acid and can regulate the balance of intestinal flora; phytase can decompose phytic acid and release mineral substances such as phosphorus, calcium and zinc; amylase can hydrolyze starch into soluble sugar, so that the energy utilization rate is increased; trypsin is used for degrading protein into small peptide and amino acid, is adaptive to the high-protein requirement of bred pigs, and can degrade cell wall polysaccharide of plant feed, reduce chyme viscosity and release embedded nutrients together with NSP enzyme; the invention also discloses an application of the breeding feed composition for pigs in breeding pig products.

The invention discloses application of a flammulina velutipes transcription factor gene AreA in regulation and control of cell wall polysaccharide synthesis and sporocarp taste. Flammulina velutipes as an important edible mushroom is widely welcomed due to rich nutrition, but the edible experience of consumers is seriously influenced and the market potential of the flammulina velutipes is limited due to the problems that the flammulina velutipes is poor in chewiness, easy to plug teeth and the like. The silent strain of the enoki mushroom areA gene is successfully constructed through an RNA interference technology, it is found that the polysaccharide content of cell walls and the toughness of sporocarp can be reduced through reduction of gene expression, then the taste of the sporocarp is improved, and the market competitiveness of the sporocarp is improved. According to the invention, the areA gene in the flammulina velutipes is effectively silenced through the specifically designed siRNA fragment, so that the polysaccharide content of the cell wall and the toughness of the sporocarp are reduced, and a theoretical basis is provided for reducing the toughness of the cell wall of the flammulina velutipes by using a genetic engineering technical means.

The invention discloses a preparation method of a physical adsorption and biodegradation composite mildew removing agent. The preparation method comprises the following steps: preparing modified montmorillonite: modifying sodium montmorillonite by using hexadecyl trimethyl ammonium bromide; culturing a bacillus subtilis fermentation culture: culturing bacillus subtilis CICC20683 by adopting a two-step method of seed culture and fermentation culture, and collecting thalli; culturing a lactic acid bacteria fermentation culture: culturing lactic acid bacteria CICC6078 by adopting a two-step method of seed culture and fermentation culture, and collecting thalli; and composite preparation: uniformly mixing 30-50 parts by weight of modified montmorillonite, 10-20 parts by weight of yeast cell wall polysaccharide, 20-30 parts by weight of bacillus subtilis fermentation culture, 10-20 parts by weight of lactic acid bacteria fermentation culture, 5-10 parts by weight of astragalus polysaccharide and 3-5 parts by weight of vitamin C, carrying out vacuum drying at 40 DEG C until the moisture content is lower than 8%, and crushing to 80 meshes. The preparation method is simple in process and convenient to operate, the used raw materials and equipment are common industrial raw materials and equipment, the production cost is low, and the preparation method has a good market application prospect.

Provided is a vegetable milk having a reduced pink feeling and a changed taste. The present technology provides: an enzyme agent for reducing the pink feeling of a plant food or beverage or a plant food or beverage material, which contains a cell wall polysaccharide-degrading enzyme; and a plant food or beverage or a plant food or beverage material, which uses the enzyme agent for reducing the pink feeling. Also provided are: a method for producing a plant food or beverage or a plant food or beverage material, said method comprising a step for causing a cell wall polysaccharide-decomposing enzyme to act on a plant raw material; and a method for reducing the pink feeling of a plant food or beverage or beverage material.

The invention relates to the technical field of glycopeptide preparation, and particularly provides a preparation method of soluble edible and medicinal fungus beta-glucan. Comprising the following steps: S1, carrying out pretreatment on liquid fermented mycelia, sporocarps of artificially cultivated edible and medicinal fungi or artificially cultivated mushroom bran mycelia; S2, carrying out enzymolysis on materials generated by pretreatment by adopting a protease reaction solution, depolymerizing the compact mycelia of the edible and medicinal fungi, sporocarps of the edible and medicinal fungi and cell wall proteoglycan protein complex tissues of the mycelia in the mushroom bran; s3, processing materials generated by enzymolysis; s4, carrying out enzymolysis on the proteoglycan protein compound suspension obtained in S3 by adopting a polysaccharide hydrolase reaction solution; according to the invention, an enzyme engineering technology and a modern separation technology are provided, macromolecular proteoglycan which is difficult to absorb and utilize is changed into smaller molecular glycopeptide, functional components in the mushroom liquid fermentation liquor are increased, and the content of effective components such as beta-glucan is increased.

[Problems] To provide plant-base milk with reduced powdery feel and altered taste.[Solution] The present technique provides an enzyme agent for reducing the powdery feel of a plant-base beverage or food or a plant-base beverage or food material, which contains a cell wall polysaccharide degrading enzyme, and a plant-base beverage or food or a plant-base beverage or food material using the enzyme agent for reducing powdery feel. The present technique also provides a method for producing a plant-base beverage or food or a plant-base beverage or food material, and a method for reducing the powdery feel of a plant-base beverage or food or a plant-base beverage or food material, which include a step of allowing a cell wall polysaccharide degrading enzyme to act on a plant-base raw material.

The invention relates to a compound feed additive used in the laying period of breeding hens and a preparation method thereof, and belongs to the technical field of breeding hens breeding and hatching. The invention discloses a compound feed additive. Comprising the following components in parts by weight: 15 to 25 parts of clostridium butyricum, 25 to 35 parts of bacillus subtilis, 12 to 18 parts of bacillus licheniformis, 5 to 15 parts of vitamin B family, 2 to 4 parts of choline dihydrogen citrate, 2 to 5 parts of herba epimedii extract, 0.2 to 0.4 part of nano L-selenomethionine, 4 to 6 parts of bile acid, 5 to 8 parts of yeast cell wall polysaccharide, 2 to 5 parts of organic zinc, 5 to 15 parts of trace elements and 1 to 3 parts of phytase. Through scientific proportioning and synergistic effect of multiple functional components, the feed improves intestinal health of breeding hens, improves reproductive performance, enhances immunity, promotes digestion and absorption of the breeding hens to nutrient substances in the feed, improves feed conversion rate and reduces feed-egg ratio.

The invention relates to the technical field of feed additives, in particular to a feed additive for improving egg shell quality of laying hens and a preparation method thereof. The feed additive for improving the egg shell quality of the laying hens is prepared from the following components in parts by weight: 2 to 4 parts of taurine, 3 to 5 parts of radix angelicae sinensis, 3 to 5 parts of herba leonuri, 1 to 3 parts of iron glycinate, 2 to 3 parts of manganese glycinate, 0.5 to 1 part of zinc methionine, 0.1 to 0.4 part of selenium yeast, 0.1 to 0.2 part of 25-hydroxyD3, 3 to 5 parts of bile acid, 2 to 5 parts of enterococcus faecium, 1 to 2 parts of yeast cell wall polysaccharide, 2 parts of compound enzyme and 20 to 30 parts of montmorillonite. 40-50 parts of stone powder, 1 part of ethoxyquin and 10 parts of rice husk powder.

The invention discloses phycobiliprotein and / or red algae oligosaccharide extracted based on a biological soluble wall and a preparation method of the phycobiliprotein and / or the red algae oligosaccharide, and belongs to the technical field of biological functional component extraction. The invention provides a preparation method for simultaneously extracting phycobiliprotein and phycobiliprotein of red algae, which is high in efficiency, low in loss rate and suitable for process amplification, specifically, cell wall polysaccharide in the red algae is matched with a compound enzyme in a matching manner, and multiple enzymes are cooperated, so that the contradiction problem between high-temperature dissolution of the cell wall polysaccharide of the red algae and heat sensitivity of phycobiliprotein is effectively solved, and the yield of the phycobiliprotein and the oligosaccharide of the red algae is improved. The wall breaking efficiency is improved, and cell wall breaking is promoted to form porous and macroporous cell wall structures; the outward migration of intracellular substances such as phycobiliprotein is promoted, so that higher extraction efficiency is obtained. According to the preparation method provided by the invention, the extraction amount of phycobiliprotein reaches 224 g / kg after production conversion, and the dissociation loss of phycobiliprotein polymers is effectively reduced. In addition, the phycobiliprotein is efficiently extracted, meanwhile, the red algae oligosaccharide product is obtained, and the process economic benefit and the raw material utilization rate are improved. The preparation method provided by the invention does not need special equipment, is simple and convenient in process, green, environment-friendly, low in cost and beneficial to industrial production.

Provided is an intestinal bacterium for protecting intestinal barrier function by including Bifidobacterium adolescentis as an active ingredient, which exhibits higher efficacy than conventional strains, wherein as an active component of the intestinal barrier protective agent, there is a cell wall polysaccharide fraction and / or cytoplasmic fraction of Bifidobacterium adolescentis, comprising a polysaccharide having a repeating structure of rhamnose, glucose, and galactose at a ratio of 4-3:1:2-1, fractionated using an ultrafiltration membrane to obtain molecules with a molecular weight of 10,000-30,000, or uridine diphosphate-N-acetylmuramic acid-L-alanine-D-glutamic acid-L-lysine.

The invention provides a method for green extraction of waste beer yeast cell wall polysaccharide by using a deep eutectic solvent, relates to the field of biomass resource utilization, and aims to solve the problems of environmental pollution, high cost and insufficient resource utilization in the prior art. The method comprises the following steps: pretreating a yeast raw material and a deep eutectic solvent to selectively dissolve mannan; performing solid-liquid separation for the first time to obtain insoluble beta-glucan and a first supernatant containing mannan; an anti-solvent is added into the supernate for precipitation, and mannan is obtained through secondary solid-liquid separation; and finally, treating the separated liquid to recover the eutectic solvent. The two polysaccharides are cooperatively extracted, the solvent is recycled, comprehensive utilization of resources is achieved, and the process is environmentally friendly, low in cost and high in efficiency.

The invention provides a combined prebiotic preparation and application thereof, and belongs to the technical field of feed additives. The combined prebiotic preparation comprises the following components in percentage by weight: 87-95% of prebiotics, 1-3% of astragalus membranaceus, 1-3% of bighead atractylodes rhizome and 1-3% of liquorice. The prebiotics are one or a mixture of more of fructo-oligosaccharide, galactooligosaccharide and yeast cell wall polysaccharide according to an equal ratio, the flavoring agent is one or a mixture of more of banana essence, meat essence and citral according to an equal ratio, the sweetening agent is one or a mixture of more of saccharin sodium and neotame according to an equal ratio, and the sweetening agent is one or a mixture of more of citric acid sodium and neotame according to an equal ratio. The prepared combined prebiotic preparation is used for mixing feed and feeding livestock or poultry, has the effects of promoting growth and development, improving the gastrointestinal environment, enhancing disease prevention and control and the like, and achieves the purposes of promoting the growth of bred animals and improving the health level.

The invention belongs to the technical field of sweet tea residue recovery, and particularly relates to a recovery process of polysaccharide in sweet tea residues and application of the recovery process. The process for recovering the polysaccharide in the sweet tea residues comprises the following steps: pretreating the sweet tea residues, performing enzymolysis treatment, and performing separation and purification treatment; the pretreatment comprises deep-eutectic solvent and pulsed electric field treatment; the eutectic solvent comprises choline chloride, chlorinated ethanolamine sodium hydrochloride and lactic acid. According to the recovery process of the polysaccharide in the sweet tea residues, provided by the invention, pretreatment comprises deep-eutectic solvent and pulsed electric field treatment, so that the deep-eutectic solvent permeates into cell structures in the sweet tea residues, and the cell structures are swelled; reversible micropores can be formed in cell membranes and cell walls of the strigose hydrangea leaf residues by applying the pulsed electric field, so that the permeability is greatly improved, the deep eutectic solvent can act deeper, then weak bonds of polysaccharide of the cell walls are broken, and the yield of the polysaccharide is further improved (19% or above).

The present application relates to the technical field of lithium metal battery, in particular to a current collector, a preparation method and application thereof, the current collector comprises a substrate, the surface of the substrate is coated with a coating layer, the coating layer comprises strawberry DNA with cell wall polysaccharide attached; the preparation method comprises the following steps: S1, preparing a second mixed solution containing strawberry DNA; S2, preparing a dry mixture from the second mixed solution; S3, weighing the dry mixture, adding Tris buffer solution, adjusting pH with the Tris buffer solution, and configuring into a third mixed solution; S4, centrifugal rotation of the third mixed solution in a centrifuge, and taking supernatant as a coating solution; S5, dropping the coating solution on the surface of the polished substrate, and baking in an oven to coat the substrate surface, obtaining the current collector; the current collector can not only effectively inhibit lithium dendrite growth and greatly reduce the short circuit risk of lithium metal battery, but also significantly improve the stability and coulomb efficiency of the battery, further improving the performance of the battery.

The invention discloses a method for evaluating the content of yeast cell wall polysaccharide in a saccharomyces cerevisiae culture, and belongs to the technical field of polysaccharide content determination. The method for evaluating the content of the yeast cell wall polysaccharide in the saccharomyces cerevisiae culture comprises the following steps: S1, sufficiently and uniformly mixing a sample with hydrochloric acid, and performing high-pressure hydrolysis; s2, carrying out derivatization treatment on the hydrolysate by using 5-methyl-2-phenyl-1, 2-dihydropyrazole-3-ketone, and carrying out hydrolysis treatment on the hydrolysate by using the 5-methyl-2-phenyl-1, 2-dihydropyrazole-3-ketone; s3, detecting mannose, glucose and galactose in the derivative sample by using a high performance liquid chromatography-tandem mass spectrometry method; and S4, according to a calibration formula provided by the distribution characteristics of the hydrolyzed mannose and the hydrolyzed galactose in the yeast and the raw materials, obtaining the content of the mannose and the beta-glucan. The method provided by the invention can effectively overcome the interference of polysaccharides from other sources, more accurately evaluate the content of active polysaccharides from the yeast cell wall in the yeast culture, and provide effective technical support for quality control, detection and evaluation of a yeast culture product.

The invention relates to the field of fruit extracts, in particular to a preparation method for obtaining a fruit extract from whole fruits, which comprises the following steps: S1, pulping: pulping cleaned whole fruits to obtain fruit pulp; s2, mixing: adding pectinase and cellulase into the fruit pulp to start enzymolysis, and performing ultrasonic treatment on the mixed pulp to strengthen mass transfer of enzyme molecules and accelerate degradation of cell wall polysaccharides; s3, distilling, namely distilling the slurry subjected to ultrasonic treatment; s4, blanking is conducted, distilled distillate is extracted, and the fruit extract is obtained.The problems that in a traditional mode, the fragrance and fragrance concentration of the fruit extract are not high, and the fragrance is not lasting are solved, and the effect of improving the fragrance, the fragrance concentration and the lasting fragrance of the fruit extract can be achieved.

This application discloses a polysaccharide for the cell wall of Citrus reticulata peel, its preparation method, and its application. The preparation method of the polysaccharide includes: weighing and drying Citrus reticulata peel, pulverizing, and sieving; extracting the Citrus reticulata peel powder by reflux in an organic solvent, washing the filter residue to remove fat, and then removing starch; washing and drying the filter residue after starch removal to obtain Citrus reticulata peel cell wall material; extracting the Citrus reticulata peel cell wall material with an extraction solution, adjusting the pH of the extraction solution to neutral, concentrating, dialyzing, and freeze-drying to obtain the polysaccharide. The Citrus reticulata peel cell wall polysaccharide is prepared by this method. The application of the Citrus reticulata peel cell wall polysaccharide is its use in the preparation of skin care products or pharmaceuticals that have antioxidant properties and promote skin damage repair.

The application discloses a cassava whole cell powder preparation and main food replacement method, and belongs to the field of special diet food development. 2+ The intermediate thin layer adhered between cell clusters is decomposed by pectinase to prepare single and complete cassava cells, and Ca The solution is used for impregnating cells to improve the binding capacity between cell wall polysaccharide components, strengthen the cell wall, and then the cell wall is used as a main raw material to be compounded with rice protein powder and crushed rice powder, so that a cassava whole cell powder recombined rice is prepared by extrusion technology.

The invention relates to the technical field of Laifeng ginger planting, and specifically provides a Laifeng ginger optimal harvesting time determination method based on texture characteristics and cell wall polysaccharides, and the method comprises the following steps: S1, time node determination: starting harvesting from the 60th day after sowing, then harvesting for one period every 30-60 days, and at least harvesting for four periods; s2, detection: selecting tender ginger of Laifeng ginger harvested at different periods in the step S1, and detecting the brittleness value, the moisture content, the transverse relaxation time of bound water, the total pectin content and the cellulose content; s3, single score conversion: converting the detection values into corresponding single scores according to a preset five-level segmentation scoring rule; and S4, comprehensively judging different brittleness harvesting periods by adopting a weighted scoring function. According to the method, a three-in-one harvesting standard of time nodes, quantitative indexes and visual characteristics is formed, traditional experience dependence is broken, technical support is provided for large-scale and standardized planting, and standardized development of the Laifeng ginger industry is promoted.

The invention discloses kluyveromyces marxianus high-yield cell wall polysaccharide and application thereof in improvement of colitis, and belongs to the technical field of fungal polysaccharide. Experiments prove that the Kluyveromyces marxianus JSA16 separated from the traditional fermented yak milk has good adaptive capacity to environment and can produce cell wall polysaccharide at high yield. Through screening, when cane sugar is used as a carbon source, the sugar yield of the JSA16 is the highest and is 177.47 mg / g. Through analysis, the extracted cell wall polysaccharide is mainly composed of mannose and accounts for 88.1% of the total sugar content. Experiments prove that the polysaccharide can significantly improve the mouse colitis symptom induced by DSS, and has a wide application prospect. The invention provides a new method for clinical treatment of colitis.

The invention relates to the technical field of feed mildew removing agents, in particular to a feed mildew removing agent based on a lysozyme-chitosan compound and a preparation method of the feed mildew removing agent, and the feed mildew removing agent comprises the following raw materials: lysozyme, quaternized-beta-cyclodextrin grafted chitosan, organic modified montmorillonite, yeast cell wall polysaccharide and corn starch. According to the invention, the organic modified montmorillonite enhances the ion adsorption capacity of strong polar toxins such as aflatoxin by enlarging the interlayer spacing; the quaternized-beta-cyclodextrin grafted chitosan ingeniously fuses the characteristics of a cationic polymer and a cyclic molecule, a quaternized chitosan chain segment adsorbs various toxins through electrostatic interaction, and the problem that a traditional adsorbent is narrow in spectrum is solved; quaternized chitosan has an antibacterial property, can effectively destroy a mould cell membrane structure, and forms a double sterilization mechanism with cell wall hydrolysis of lysozyme, so that mould proliferation is inhibited from the source, and generation of toxins is reduced.

The invention relates to the technical field of lithium metal batteries, in particular to a current collector and a preparation method and application thereof.The current collector comprises a matrix, the surface of the matrix is coated with a coating layer, and the coating layer comprises strawberry DNA attached with cell wall polysaccharide; the preparation method comprises the following steps: S1, preparing a second mixed solution containing strawberry DNA; s2, preparing a dry mixture from the second mixed solution; s3, weighing the dried mixture, adding a Tris buffer solution, and adjusting the pH value by using the Tris buffer solution to prepare a third mixed solution; s4, putting the third mixed solution into a centrifugal machine for centrifugal rotation, and taking supernatant liquid as coating liquid; step S5, coating liquid is dropped on the surface of the ground substrate, and is baked in a baking oven to coat the surface of the substrate, so that the current collector is obtained; according to the current collector, the growth of lithium dendrites can be effectively inhibited, and the short-circuit risk of a lithium metal battery is greatly reduced; and the stability and coulombic efficiency of the battery can be remarkably improved, and the performance of the battery is further improved.

The present invention provides a cherry blossom fermentation product, a preparation method thereof, a composition and an application, belonging to the technical field of cosmetics. It includes: S1. Freeze the cherry blossoms in liquid nitrogen, thaw them, and break their cell walls to obtain cherry blossom powder; S2. Add the cherry blossom powder to an aqueous solution of a deep eutectic solvent for extraction, filter to obtain solid residue 1; add sodium phosphate to the filtrate for precipitation to obtain solid residue 2, and dry the filtrate to prepare an extract; S3. Culture Streptococcus thermophilus to obtain a seed solution of the strain; S4. Inoculate and ferment the seed solution of the strain, centrifuge to collect the bacterial sludge, and freeze-dry the supernatant to prepare a Streptococcus thermophilus cherry blossom ferment; S5. Enzymatically hydrolyze the bacterial sludge, break its cell walls under high pressure, add ethanol to the cell wall-breaking solution for precipitation to obtain cell wall polysaccharide, and dry the filtrate to prepare a lysate; S6. Mix the extract, the Streptococcus thermophilus cherry blossom ferment, the cell wall polysaccharide and the lysate to prepare a product, which has the effects of moisturizing and repairing, anti-glycation, antioxidant, brightening the skin, anti-aging, anti-wrinkle and anti-damage.

The present application provides a kind of cell wall polysaccharide extracted from Pu'er tea and its extraction method and application.The first aspect of the present application provides a kind of method for extracting cell wall polysaccharide from Pu'er tea, first remove water-soluble polysaccharide and macromolecular substances such as protein;Second, a certain amount of amylase is used to further process the non-water-soluble residue to remove starch;Then, ethylenediaminetetraacetic acid combined with alkali solution is used to extract acidic polysaccharide, and it is found through test that the cell wall polysaccharide extracted by the method has immunoregulatory effect, and the activity is higher than that of polysaccharide extracted by traditional method.Therefore, by the method provided by the present application, the cell wall polysaccharide in Pu'er tea is developed and utilized, which helps to improve the industrial added value, broaden the product category, has higher application prospect and potential value.

The application discloses a preparation method of cassava whole cell powder special-diet ingredient, and belongs to the field of special-diet food development. The application provides a kind of anti-digestion cassava whole cell powder special-diet food ingredient, which utilizes pectinase to enzymatically hydrolyze and separate cassava tuber tissues, to prepare single complete cassava cells, and utilizes the non-covalent force between cell wall polysaccharides and phenolic substances to promote the cell wall to adsorb phenolic active substances, to enhance the strength of the cell wall and the inhibitory effect on digestive enzymes, and at the same time, the cassava whole cell powder is treated by wet heat, to promote the rearrangement of intracellular starch molecular chains of the cassava whole cell, to further enhance the anti-digestion capacity of the starch.