Combined prebiotic preparation and application thereof

Abstract

The invention provides a combined prebiotic preparation and application thereof, and belongs to the technical field of feed additives. The combined prebiotic preparation comprises the following components in percentage by weight: 87-95% of prebiotics, 1-3% of astragalus membranaceus, 1-3% of bighead atractylodes rhizome and 1-3% of liquorice. The prebiotics are one or a mixture of more of fructo-oligosaccharide, galactooligosaccharide and yeast cell wall polysaccharide according to an equal ratio, the flavoring agent is one or a mixture of more of banana essence, meat essence and citral according to an equal ratio, the sweetening agent is one or a mixture of more of saccharin sodium and neotame according to an equal ratio, and the sweetening agent is one or a mixture of more of citric acid sodium and neotame according to an equal ratio. The prepared combined prebiotic preparation is used for mixing feed and feeding livestock or poultry, has the effects of promoting growth and development, improving the gastrointestinal environment, enhancing disease prevention and control and the like, and achieves the purposes of promoting the growth of bred animals and improving the health level.

Description

Technical Field

[0001] The invention belongs to the technical field of feed additives and relates to a combined prebiotic preparation and application thereof. Background Art

[0002] In recent years, as the use of chemical drugs such as antibiotics has caused drug resistance and residue problems, the demand for safe, environmentally friendly and efficient natural additives in the animal feed field has continued to grow. In this context, prebiotics and Chinese herbal medicines, as green and harmless additives, have attracted much attention because of their ability to regulate intestinal flora, promote healthy development, and improve feed utilization and weight gain effects in farmed animals.

[0003] Prebiotics are a type of functional ingredient that is not digested and absorbed by the host. They improve the overall health of animals by promoting the proliferation of beneficial bacteria in the intestines. The benefits of prebiotics include the following aspects: 1. Improve intestinal health: Prebiotics can promote the growth of beneficial bacteria, inhibit harmful bacteria, balance intestinal microorganisms, maintain intestinal health, help reduce digestive problems such as diarrhea, and improve the health of the animal's digestive system; 2. Enhance immunity: Prebiotics can indirectly stimulate the immune system, enhance the animal's disease resistance, and reduce the incidence of diseases by improving the intestinal environment. Prebiotics can activate local immunity and help animals better resist pathogens; 3. Improve feed conversion rate: By improving intestinal flora, prebiotics can promote the absorption and utilization of nutrients, improve feed conversion efficiency, and increase weight gain; 4. Reduce dependence on antibiotics: Prebiotics are a safe alternative to antibiotics that can effectively reduce dependence on antibiotics and reduce the risk of drug-resistant bacteria.

[0004] As natural feed additives, Chinese herbal medicine has the advantages of promoting animal health, improving intestinal health, increasing growth rate, containing no antibiotic residues, and being environmentally friendly. Astragalus is rich in active ingredients such as polysaccharides, saponins, and flavonoids, and has the effects of enhancing immunity, anti-oxidation, and anti-inflammatory. It can improve the disease resistance of animals and reduce the risk of infection. Atractylodes macrocephala is known for its ability to regulate the spleen and stomach and improve the function of the digestive system. It can promote appetite and improve the digestion and absorption rate of feed. Licorice has a broad spectrum of antibacterial, anti-inflammatory, detoxifying and sedative effects, and can enhance the efficacy of other Chinese herbal medicine ingredients (known as "harmony of all medicines"), and can also protect the intestinal mucosa and promote intestinal health. The combination of the three Chinese herbal medicines can achieve mutual promotion of multiple effects and meet the needs of healthy, safe and sustainable breeding. The combined use of Chinese herbal medicines and prebiotics is conducive to covering up their own bad odors and increasing feed intake.

[0005] Therefore, the present application proposes a combination of prebiotics and Chinese herbal medicines through a reasonable formula and ingredient ratio, which can be added to feed to effectively improve the growth of farmed animals. Summary of the invention

[0006] The purpose of the present invention is to address the above-mentioned problems in the existing technology and propose a combined prebiotic preparation and its application. The prebiotic preparation prepared by combining prebiotics with Chinese herbal medicine can effectively promote weight gain and improve the health level of farmed animals.

[0007] The purpose of the present invention can be achieved by the following technical solutions:

[0008] A combined prebiotic preparation, composed of a combination of prebiotics and Chinese herbal medicines, characterized in that it comprises the following components and their weight percentages in the combined prebiotic preparation: 87-95% of prebiotics, 3-9% of Chinese herbal medicines, 1%-3% of flavoring agents, and 0.1-1% of sweeteners.

[0009] Preferably, the prebiotics are one or more of fructooligosaccharides, galacto-oligosaccharides and yeast cell wall polysaccharides mixed in equal proportions.

[0010] Preferably, the Chinese herbal medicine is astragalus, atractylodes macrocephala, and liquorice, and the weight percentage of the combined prebiotic preparation is: 1%-3% astragalus, 1%-3% atractylodes macrocephala, and 1%-3% liquorice.

[0011] Preferably, the flavoring agent can be a mixture of one or more of banana flavor, meat flavor, and citral in equal proportions.

[0012] Preferably, the sweetener may be a mixture of one or more of saccharin sodium and neotame in equal proportions.

[0013] Preferably, the components of the combined prebiotic preparation are all in powder form and are mixed.

[0014] The purpose of the present invention can also be achieved by the following technical solutions:

[0015] An application of a combined prebiotic preparation, characterized in that the prepared combined prebiotic preparation is used for mixing feed to feed livestock or poultry.

[0016] Compared with the prior art, the present invention has the following advantages:

[0017] (1) Promoting growth and development: The prebiotic ingredients in the present invention and the Chinese herbal medicine ingredients such as astragalus, white atractylodes, and liquorice have the effects of enhancing immunity and promoting growth, and can effectively increase daily weight gain and final weight.

[0018] (2) Improve gastrointestinal environment: Prebiotics and Chinese herbal ingredients can improve the digestion and absorption rate of feed by regulating intestinal flora, promoting the growth of beneficial bacteria and inhibiting harmful bacteria. This not only increases the utilization of nutrients, but also reduces feed consumption and improves economic benefits.

[0019] (3) Enhanced disease prevention and control effects: By optimizing the immune system and intestinal microecological environment, the present invention can effectively improve the antioxidant capacity of animals, effectively prevent and control the occurrence of diseases without the use of antibiotics, help reduce the incidence of common intestinal infections and respiratory diseases, and ensure the healthy growth of animals. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 1 is a Venn diagram of the operational classification units of different groups of intestinal flora in an embodiment of the present invention.

[0021] Figure 2 It is a principal coordinate analysis diagram of the intestinal flora in an embodiment of the present invention.

[0022] Figure 3 It is a stacked bar chart of relative abundance species of different groups in the intestinal flora phylum in the embodiment of the present invention.

[0023] Figure 4 It is a stacked bar chart of relative abundance species of different groups in the intestinal flora genus in the embodiment of the present invention.

[0024] Figure 5 It is a hidden Dirichlet distribution diagram of the intestinal flora in an embodiment of the present invention. DETAILED DESCRIPTION

[0025] The following are specific embodiments of the present invention and the accompanying drawings to further describe the technical solution of the present invention, but the present invention is not limited to these embodiments.

[0026] Embodiment 1

[0027] The combined prebiotic preparation comprises the following components (based on 1 kg): 488.7 g yeast cell wall polysaccharide, 450 g fructooligosaccharide, 20 g astragalus, 20 g atractylodes, 20 g astragalus, 1 g flavoring agent, and 0.3 g sweetening agent. Each component is mixed in powder form, meat flavoring agent is selected as the flavoring agent, and saccharin sodium is selected as the sweetening agent.

[0028] Embodiment 2

[0029] like Figure 1-2 As shown, the combined prebiotic preparation is used in mixed feed for feeding livestock or poultry, which can effectively promote weight gain and improve the health level of farmed animals.

[0030] The experiment was conducted with mice as the target:

[0031] A control group, a low-dose group, a medium-dose group and a high-dose group were set up. The control group was fed with a basic diet, and the low-dose group, the medium-dose group and the high-dose group were fed with 10 mg / kg, 20 mg / kg and 40 mg / kg of the above test sample on the basis of the basic diet, mixed evenly and fed. Each group was repeated 8 times and fed continuously for 16 weeks. During the feeding period, the mice were free to eat and drink water. The temperature of the mouse room was maintained at 25±1℃, the relative humidity was 45-55%, and the light and dark alternation time was 12 / 12h. After 16 weeks, the growth performance, antioxidant capacity and cecal microbial indicators of each group were measured.

[0032]

[0033] Table 1 Results of mouse growth performance measurement

[0034] Different lowercase and uppercase letters in the same column indicate statistically significant differences among the groups (P<0.05, P<0.01), the same below.

[0035]

[0036]

[0037] Table 2 Results of mouse organ index determination

[0038] Group Control group Low dose Medium dose High dose <![CDATA[MDA / (μmol·L -1 )]]> 18.40±3.03 18.30±1.74 17.48±3.22 17.21±4.42 <![CDATA[SOD / (U·mL -1 )]]> <![CDATA[143.13±17.60 B ]]> <![CDATA[171.75±16.81 A ]]> <![CDATA[106.69±15.67 C ]]> <![CDATA[113.50±7.37 C ]]> <![CDATA[ALT activity / (U·L -1 )]]> 16.19±3.13 15.72±3.17 16.13±3.19 16.98±2.81 <![CDATA[AST activity / (U·L -1 )]]> <![CDATA[26.82±6.06 A ]]> <![CDATA[18.88±4.00 B ]]> <![CDATA[21.24±2.43 B ]]> <![CDATA[24.06±8.35 AB ]]>

[0039] Table 3 Results of the determination of antioxidant capacity in mice

[0040] As shown in Table 1, from the performance of final body weight and average daily weight gain, the low-dose group>high-dose group>medium-dose group>control group, among which the final body weight and average daily weight gain of the low-dose group increased significantly (P<0.05), which increased by 11.3% and 51.5% respectively compared with the control group, and the average daily feed intake and feed-to-weight ratio performed well. The medium-dose group and the high-dose group had no significant effect on the growth performance of mice (P>0.05). Observation of the feed-to-weight ratio and average daily feed intake shows that the combined prebiotic preparation achieves weight gain by improving feed utilization.

[0041] As shown in Table 2, there was no significant difference in cardiac index and renal index among the groups (P>0.05); the liver index of the high-dose group was significantly lower than that of the other groups (P<0.05); the spleen index of the low-dose group and the high-dose group was significantly (P<0.05) lower than that of the control group and the medium-dose group.

[0042] As shown in Table 3, there was no significant difference in MDA and ALT concentrations among the groups (P>0.05); T-SOD concentration in the low-dose group was significantly higher than in other groups (P<0.01), and in the medium-dose and high-dose groups was significantly lower than in the control group (P<0.01), and AST activity in the low-dose and medium-dose groups was significantly lower than in the control group (P<0.01). The low-dose combined prebiotic preparation relieves oxidative stress by enhancing the body's SOD enzyme activity, thereby improving the body's antioxidant capacity in mice.

[0043] Group Control group Low dose group Medium dose group High dose group Chao1 Index <![CDATA[259.29±21.35 B ]]> <![CDATA[312.24±13.82 A ]]> <![CDATA[285.77±14.67 AB ]]> <![CDATA[293.02±27.82 AB ]]> ACE Index <![CDATA[257.06±18.34 Bc ]]> <![CDATA[305.67±9.78 Aa ]]> <![CDATA[281.02±13.54 ABb ]]> <![CDATA[285.45±15.40 ABab ]]> Shannon Index 3.43±0.94 3.84±0.42 3.44±0.38 3.80±0.26 Simpson Index 0.87±0.13 0.94±0.23 0.92±0.03 0.95±0.02

[0044] Table 4 Results of intestinal flora α diversity determination

[0045] Chao1 index and ACE index are used to describe the richness of microbial colonies in samples, while Shannon index and Simpson index are used to describe the diversity of microbial colonies in samples. As shown in Table 4, Chao1 index and ACE index of the low-dose group were significantly higher (P<0.01) than those of the other three groups, while Shannon index and Simpson index were not significantly different among the groups (P<0.05). The distance between groups in the principal coordinate diagram reflects the degree of similarity between groups. Figure 2 It can be seen that the medium-dose group is closer to the control group, while the low-dose group is farther away from the control group, reflecting the large difference in the community composition between the two.

[0046]

[0047] Table 5 The bacterial phyla with large differences and their relative abundance

[0048]

[0049] Table 6 The bacterial phyla with large differences and their relative abundance

[0050] Depend on Figure 3 It can be seen that, specifically at the phylum level, the top four phyla in the control group, medium-dose group and high-dose group were Firmicutes, Bacteroidetes, Desulfobacteria and Verrucomicrobia; the top four phyla in the low-dose group were Firmicutes, Bacteroidetes, Desulfobacteria and Actinobacteria. As shown in Table 5, compared with the control group, the relative abundance of Verrucomicrobia in the high-dose group was extremely significantly (P<0.01) decreased; the Actinobacteria in the low-dose group was extremely significantly (P<0.01) increased.

[0051] Depend on Figure 4 It can be seen that at the genus level, the top four bacterial genera in the control group, low-dose group, medium-dose group and high-dose group were Staphylococcus, Desulfovibrio, unclassified_f_Lachnospiraceae and uncultured_f_Lachnospiraceae.

[0052] As shown in Table 6, compared with the control group, the relative abundance of Bifidobacterium and Faecalibacterium in the low-dose group was significantly increased (P<0.01), and the relative abundance of Akkermansia muciniphila in the high-dose group was significantly increased (P<0.01).

[0053] Compared with the control group, the low-dose group significantly increased the relative abundance of Bifidobacterium and Faecalibacterium prausnitzii, among which the relative abundance of Bifidobacterium increased from 0.00% to 5.82%, and the relative abundance of Faecalibacterium prausnitzii increased from 0.00% to 8.73%. This result shows that the low-dose combined prebiotic preparation has a significant promoting effect on the proliferation of these two types of beneficial bacteria. Bifidobacterium is one of the important probiotics in the intestine. It can effectively reduce the pH value of the intestine by producing metabolites such as organic acids (such as lactic acid and acetic acid) and bacteriocins, thereby inhibiting the growth of harmful bacteria. In addition, Bifidobacterium also has the ability to decompose cellulose and protein, thereby improving the intestinal microenvironment, enhancing digestion and absorption function, and promoting intestinal peristalsis. Faecalibacterium prausnitzii is a common beneficial microorganism in the intestine and is also the main butyrate-producing bacteria. Butyrate, as a short-chain fatty acid, can maintain the intestinal barrier function, enhance the integrity of the intestinal mucosa, and has a significant anti-inflammatory effect. In addition, the abundance of Faecalibacterium prausnitzii is closely related to the diarrhea rate and weight gain level of animals. The higher the abundance, the faster the weight gain, and the lower the incidence of diarrhea. This test case not only optimizes the intestinal flora structure by directed proliferation of Bifidobacterium and Faecalibacterium prausnitzii, but also indirectly improves the growth performance of animals by improving the intestinal environment and function. In summary, this combination of prebiotic preparations is used in mixed feeds and has been verified on mice to improve feed utilization. All doses have been shown to promote weight gain and improve health levels in farmed animals.

[0054] The specific embodiments described herein are merely examples of the spirit of the present invention. Those skilled in the art may make various modifications or additions to the specific embodiments described or replace them in similar ways, but they will not deviate from the spirit of the present invention or exceed the scope defined by the appended claims.

Claims

1. A combined prebiotic preparation, composed of a combination of prebiotics and Chinese herbal medicines, characterized in that: The said prebiotic preparation comprises the following components and their weight percentages in combination: 87-95% of prebiotics, 3-9% of Chinese herbal medicine, 1%-3% of flavoring agent, and 0.1-1% of sweetener.

2. The combined prebiotic preparation according to claim 1, characterized in that The prebiotics are one or more of fructooligosaccharides, galacto-oligosaccharides and yeast cell wall polysaccharides mixed in equal proportions.

3. The combined prebiotic preparation according to claim 1, characterized in that The Chinese herbal medicines are astragalus, atractylodes macrocephala and liquorice, and the weight percentage of the combined prebiotic preparation is: 1%-3% of astragalus, 1%-3% of atractylodes macrocephala and 1%-3% of liquorice.

4. The combined prebiotic preparation according to claim 1, characterized in that The flavoring agent is a mixture of one or more of banana flavor, meat flavor and citral in equal proportions.

5. The combined prebiotic preparation according to claim 1, characterized in that The sweetener is a mixture of one or more of saccharin sodium and neotame in equal proportions.

6. The combined prebiotic preparation according to claim 1, characterized in that The components of the combined prebiotic preparation are all in powder form and are mixed.

7. A use of a combined prebiotic preparation according to any one of claims 1 to 6, characterized in that: The prepared combined prebiotic preparation is used for mixing feed and feeding livestock or poultry.

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