A kind of preparation method of ferrous chelating antimicrobial peptide

Abstract

The invention discloses a preparation method for a ferrous chelated antibacterial peptide, especially to a preparation method for a hairtail enzymatic hydrolysis protein ferrous chelated antibacterial peptide. The method comprises the following steps: 1) adding leftovers of hairtail into distilled water, then adding papain for enzymatic hydrolysis and adjusting the pH value of enzymatic hydrolysis liquid to be 5.5 to 6.5; and 2) adding ferrous chloride into the enzymatic hydrolysis liquid, carrying out oscillating chelation for 30 to 60 min at a chelation temperature of 30 to 50 DEG C, then carrying out centrifugation and removing a precipitate so as to obtain a crude extract of the hairtail enzymatic hydrolysis protein ferrous chelated antibacterial peptide. To obtain a refined product of the hairtail enzymatic hydrolysis protein ferrous chelated antibacterial peptide, the crude extract undergoes the following purification steps: 1) freeze drying the crude extract of the hairtail enzymatic hydrolysis protein ferrous chelated antibacterial peptide, eluting and separating the crude extract by using sephadex chromatography, subjecting chromatographic components with antimicrobial activity to reverse high performance liquid chromatographic elution and collecting eluate; and 2) subjecting the eluate to vacuum freeze drying so as to obtain the hairtail enzymatic hydrolysis protein ferrous chelated antibacterial peptide. The method provided by the invention has the characteristics of simple operation, a high separation and recovery rate and low cost and enables high value utilization of waste produced in processing of hairtail to be realized.

Description

technical field

[0001] The invention relates to a preparation method of a ferrous chelating antibacterial peptide, in particular to a preparation method of a hairtail proteolytic hydrolysis of a ferrous chelating antibacterial peptide. Background technique

[0002] Hairtail is one of the most important marine economic fish in our country. Hairtail is rich in nutrition and delicious. In addition to being eaten directly, hairtail can also be processed into canned food and instant food. However, the head, bones, and viscera of hairtail will be discarded during processing. If not used, this part accounts for more than half of the weight of hairtail. At present, the utilization of these leftovers is mostly focused on the production of animal feed, fish sauce, hydrolyzed protein, etc., but these leftovers are rich in unsaturated fatty acids and high-quality protein resources. Such processing methods not only waste high-quality protein resources, but also increase production costs....

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