Method for extracting microbial oil

The technology of a microbial oil and an extraction method, which is applied to the field of microbial oil extraction, can solve the problems of high time consumption, high energy consumption, reduction of target fatty acid content, etc., achieves large-scale production, is beneficial to large-scale production, and improves oil extraction efficiency. Effect

Active Publication Date: 2011-03-16
CABIO BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the technology of using microbial fermentation to produce polyunsaturated fatty acid oil is very mature, but there are still some problems in the extraction process of microbial oil, which needs to be improved
[0004] The patent application of CN101133144A discloses the use of plant seeds and organisms to mix and dry to obtain oils containing unsaturated fatty acids by pressing. The main problem of this process is that the fatty acids in the plant seeds themselves reduce the content of target fatty acids. The outer wall of somatic cells is relatively hard, and the actual oil extraction effect is not optimistic
[0005] The patent application of CN1217029A discloses using the method of preparing

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) After inoculating the slant with dinoflagellate strains, carry out liquid shake flask culture, and then transfer to a 70L automatic fermenter, wherein the composition of the medium is: tryptone 1g / L, yeast extract 2g / L L, glucose 30g / L, KH 2 PO 4 3g / L, NaCl 30g / L, MgSO 4 .7H 2 O 10g / L, KCl 1g / L, CaCl 2 0.3g / L, NaHCO 3 1g / L, FeSO 4 0.01g / L, glutamic acid 40g / L, the rest is water, adjust the pH value of the medium to 6~8, maintain the temperature of the fermenter at 25~28°C, and the air flow per minute is 0.8 times the volume of the fermentation broth , cultivated for 126h, and the fermentation was completed to obtain a fermented liquid;

[0030] (2) Adjust the temperature of the fermentation broth to 40°C, adjust the pH value of the fermentation broth to 3.5 with phosphoric acid, and continue stirring for 6 hours to autolyse the microbial cells in the fermentation broth to obtain 50L of fermentation broth;

[0031] (3) Take 50L of fermentation broth obtain...

Embodiment 2

[0037] (1) After inoculating the slant with dinoflagellate strains, carry out liquid shake flask culture, and then transfer to a 70L automatic fermenter, wherein the composition of the medium is: tryptone 1g / L, yeast extract 2g / L L, glucose 30g / L, KH 2 PO 4 3g / L, NaCl 30g / L, MgSO 4 .7H 2 O 10g / L, KCl 1g / L, CaCl 2 0.3g / L, NaHCO 3 1g / L, FeSO 4 0.01g / L, glutamic acid 40g / L, the rest is water, adjust the pH value of the medium to 6~8, maintain the temperature of the fermenter at 25~28°C, and the air flow per minute is 0.8 times the volume of the fermentation broth , cultivated for 126h, and the fermentation was completed to obtain a fermented liquid;

[0038] (2) Adjust the temperature of the fermentation broth to 50°C, adjust the pH value of the fermentation broth to 3.5 with sulfuric acid, and continue stirring for 6 hours to autolyse the microbial cells in the fermentation broth to obtain 50L of fermentation broth;

[0039] (3) Take 50L of fermentation broth obtained...

Embodiment 3

[0046] (1) After the dinoflagellate ampoule tank is used to inoculate the inclined surface, the liquid shake flask culture is carried out, and then transferred to the first-level seed tank for culture, and then transferred to the 50m 3 Fermenter culture, wherein the composition of the medium is: tryptone 1g / L, yeast extract 2g / L, glucose 30g / L, KH 2 PO 4 3g / L, NaCl 30g / L, MgSO 4 .7H 2 O 10g / L, KCl 1g / L, CaCl 2 0.3g / L, NaHCO 3 1g / L, FeSO 4 0.01g / L, glutamic acid 40g / L, the rest is water, adjust the pH of the medium to 6~8, maintain the temperature of the fermenter at 25~28°C, and the air flow per minute is 1.5 times the volume of the fermentation broth , cultivated for 120h, and the fermentation was completed to obtain a fermented liquid;

[0047] (2) Adjust the temperature of the fermentation broth to 55°C, and adjust the pH value of the fermentation broth to about 4.0 with hydrochloric acid for 12 hours to autolyze the microbial cells in the fermentation broth to ob...

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Abstract

The invention provides a method for extracting microbial oil, which comprises the following steps: (1) allowing an oil-producing microbial strain to ferment and obtaining fermentation liquor; (2) regulating the pH value of the fermentation liquor obtained by the step (1) to 3.5 to 4.5, and allowing microbes to autolyze; (3) extracting by adding an extracting solvent into the fermentation liquor obtained by the step (2), and collecting upper mixed oil; (4) extracting again by adding the extracting solvent into the lower solution obtained by the extraction and separation in the step (3), and collecting upper mixed oil; and (5) mixing the upper mixed oil obtained by the step (3) and the upper mixed oil obtained by the step (4), distilling, recovering the extracting solvent and obtaining the microbial oil product. The method can extract the oil in the cells of microbes effectively, has high oil extraction rate and high oil extraction efficiency, makes process simple and is favorable for large-scale production.

Description

technical field [0001] The invention relates to a method for extracting microbial oil. Background technique [0002] Polyunsaturated fatty acids (PUFA) play a vital role in the growth and development of the human body and health. To maintain the normal functions of various tissues, the human body must ensure that there are sufficient fatty acids. If it is lacking, it will cause a series of symptoms, including growth retardation, abnormal skin scales, mental retardation, etc. For example, docosahexaenoic acid (DHA), as an important PUFA, plays a very significant role in enhancing memory, thinking ability, and improving intelligence. When the diet lacks DHA for a long time, it will have adverse effects on people's intelligence, memory ability and thinking ability. Population epidemiological studies have found that people with high DHA content in their bodies have stronger psychological endurance and higher intellectual development index. [0003] At present, the technology ...

Claims

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Application Information

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IPC IPC(8): C12P7/64C11B1/10C12R1/645C12R1/89
Inventor 尚耘汪志明马凡提向丽肖子豪唐孝鹏
Owner CABIO BIOTECH WUHAN CO LTD
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