Separation and extraction method of microbial oil

A technology of microbial oil and extraction method, which is applied in the field of microbial oil separation and extraction, can solve the problems of complex production process, low oil yield and high energy consumption, and achieves the effects of simple operation, high oil yield and simplified process steps.

Inactive Publication Date: 2008-12-17
ENERGY RES INST OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to solve the problems of complex production process, high energy consumption, high pollution and low oil yield in the prior art, and use the technology of concentrating the fermented liquid combined with high-pressure homogenization to break the cells, and then use two extractions to collect the Contains fat, and correspondingly obtains bacterial protein

Method used

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  • Separation and extraction method of microbial oil

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Experimental program
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Effect test

Embodiment 1

[0020] Using Candida terrestris as the starting strain, follow the steps below:

[0021] A, first carry out the preparation of secondary seed liquid in shake flask, then inoculate in the 30L fermentor that substratum is housed with prepared seed liquid, fermentation medium composition is yeast powder 15g / L, peptone 15g / L, Glucose 70g / L, cultured at pH 5.6, 30°C, and agitation speed of 250r / min for 7 days under aerobic conditions, and finally obtained the required fermentation broth, in which the biomass was 100g / Kg fermentation broth, containing 0.475g oil / g dry bacteria;

[0022] B, add the 5A type molecular sieve of 2.5Kg / Kg fermented liquid (Tianjin Damao Chemical Reagent Factory) in fermented liquid, remove 50% moisture in the fermented liquid to improve the concentration of thalline under stirring state, leave standstill to isolate molecular sieve , can be reused after drying;

[0023] C. Start the main motor of the high-pressure homogenizer (NS3015H, Niro Soavi Compan...

Embodiment 2

[0027] Using Candida terrestris as the starting strain, follow the steps below:

[0028] A, first carry out the preparation of secondary seed liquid in shake flask, then inoculate in the 30L fermentor that substratum is housed with prepared seed liquid, fermentation medium composition is yeast powder 15g / L, peptone 15g / L, Glucose 70g / L, cultured at pH 5.6, 30°C, and agitation speed of 250r / min for 7 days under aerobic conditions, and finally obtained the required fermentation broth, in which the biomass was 100g / Kg fermentation broth, containing 0.475g oil / g dry bacteria;

[0029] B. Add 2.5Kg / Kg 5A molecular sieve to the fermentation liquid, absorb and concentrate to remove 50% of the water in the fermentation liquid under stirring to increase the concentration of the bacteria, stand to separate the molecular sieve, and reuse it after drying ;

[0030] C. Start the main motor of the homogenizer, slowly rotate the secondary pressure regulating handle to make the final homog...

Embodiment 3

[0034] Using Candida terrestris as the starting strain, follow the steps below:

[0035] A, first carry out the preparation of secondary seed liquid in shake flask, then inoculate in the 30L fermentor that substratum is housed with prepared seed liquid, fermentation medium composition is yeast powder 15g / L, peptone 15g / L, Glucose 70g / L, cultured at pH 5.6, 30°C, and agitation speed of 250r / min for 7 days under aerobic conditions, and finally obtained the required fermentation broth, in which the biomass was 100g / Kg fermentation broth, containing 0.475g oil / g dry bacteria;

[0036] B. Add 2.5Kg / Kg 5A molecular sieve to the fermentation liquid, absorb and concentrate to remove 50% of the water in the fermentation liquid under stirring to increase the concentration of the bacteria, stand to separate the molecular sieve, and reuse it after drying ;

[0037] C. Start the main motor of the homogenizer, slowly turn the secondary pressure regulating handle to make the final homogeniz...

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Abstract

A method for separating and extracting microbial oil comprises the following steps: (1) microbial strains are inoculated for fermentation; (2) the obtained fermentation liquor is concentrated to remove 45 percent to 55 percent of water in the fermentation liquor so as to obtain the concentrated fermentaton liquor; (3) the concentrated fermentation liquor is fed into a high pressure homogenizer with the pressure of 70 to 130MPa to carry out cell-crushing; (4) an extractive solvent is added, and after two times of extraction, the upper layer organic solution phase is separated and collected to obtain mixed oil; and (5) at last, the solvent in the mixed oil is vaporized and recycled to obtain the microbial oil. The method combines the fermented solution concentration with the high pressure homogenization technology to carry out cell crushing, and then adopts the two-time extraction to collect the contained oil and obtain bacterial protein accordingly. The process improves the original method which needs the steps, such as the separation of strains, drying, grinding and granulation, etc., thus greatly simplifying the technology, improving efficiency and reducing production cost. The method homogenizes the fermented solution under high pressure after concentration, which promotes the utilization rate of facilities, and reduces energy consumption.

Description

technical field [0001] The invention relates to a separation process of a target product in the downstream process of bioengineering, in particular to a method for extracting microbial oil. Background technique [0002] Microbial oil is made by certain microorganisms, such as yeast, mold, algae, etc., under certain conditions, convert excess carbohydrates into glycerides and store them in the cells of the bacteria, and some dry bacteria can contain as much as 60% oil above. The fatty acid composition of most microbial oils is similar to that of common vegetable oils such as rapeseed oil, soybean oil, palm oil, etc. It mainly contains palmitic acid, stearic acid, oleic acid and polyunsaturated fatty acids, etc. The shortage of vegetable oil alleviates the trend that the oil market is in short supply and opens up a new path for the source of oil. [0003] Microbial oil exists in the cells and is an intracellular product. Only by using an appropriate method to initially crush...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C11B1/10C12R1/645
Inventor 张晓东李岩张杰许海朋孙立
Owner ENERGY RES INST OF SHANDONG ACAD OF SCI
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