Process for preparing microbe oil

A technology for microbial oils and oil-producing microorganisms, which is applied in the fields of biofuels, fat production, and biological raw materials to achieve the effects of low production cost, reduced raw material cost, and wide application range.

Inactive Publication Date: 2008-01-23
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no relevant report about the production of microbial oil with Jerusalem artichoke as raw material

Method used

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  • Process for preparing microbe oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Follow the following process steps to prepare microbial oil successively:

[0024] A. Raw material pretreatment

[0025] Clean the fresh Jerusalem artichoke tubers, mix them with water at a mass volume ratio of 1:1, crush them with a juice extractor, and adjust the slurry to obtain a solid-liquid mixture. Adjust the initial pH to 3.0, and sterilize at 121° C. for 15 minutes before use.

[0026] B. Acquisition of oleaginous bacteria

[0027] Rhodosporidium toruloides (Rhodosporidium toruloides) AS 2.1389 seed solution (containing 10 6 ~10 8 Cells / mL) were inserted into the solid-liquid mixture prepared according to the above-mentioned process A according to the inoculum amount of 5%, cultured with shaking at 30°C and 200r / min for 5 days, and centrifuged at 5000rpm for 10min to collect the bacterial precipitate.

[0028] C. Oil extraction

[0029] Add 5mL of 4mol / L HCl per gram of bacteria, and digest the bacteria at 70°C for 30 minutes.

[0030] After cooling, add m...

Embodiment 2

[0032] Fresh Jerusalem artichoke tubers are crushed, mixed with water at a mass-volume ratio of 1:5, crushed with a juice extractor, and slurried to obtain a solid-liquid mixture, adjusted to an initial pH of 6.0, and sterilized at 121° C. for 15 minutes before use.

[0033] Lipomyces starkeyi AS2.1608 seed solution (containing 10 6 ~10 8 Cells / mL) were inserted into the solid-liquid mixture prepared according to the above-mentioned process A according to the inoculation amount of 20%, 30°C, 200r / min shaking culture for 5 days, and centrifuged at 5000rpm for 10min to collect the bacterial precipitate.

[0034] The oil extraction process is the same as in Example 1.

[0035] According to this process condition, 100g of fresh Jerusalem artichoke can obtain 2.8g of oil.

Embodiment 3

[0037] A. Raw material pretreatment

[0038] The dried Jerusalem artichoke tubers are crushed, mixed with water at a mass volume ratio of 1:3 to obtain a solid-liquid mixture, pH 7.0, and sterilized at 121° C. for 15 minutes before use.

[0039] B. Acquisition of oleaginous bacteria

[0040] Mortierella isabellina (Mortierella isabellina) AS3.3410 seed solution (containing 10 6 ~10 8spore cells / mL) were inserted into the solid-liquid mixture prepared according to the above-mentioned A process at an inoculum size of 10%, cultured with shaking at 37°C and 220r / min for 6 days, and centrifuged at 5000rpm for 10min to collect the bacterial precipitate.

[0041] The oil extraction process is the same as in Example 1.

[0042] According to this process condition, 100g dried Jerusalem artichoke can get 10.2g oil.

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Abstract

The invention relates to a microbial oil preparation method, in particular to a microbial oil preparation method taking jerusalem artichoke tubers as the raw material. After wash, crush or slicing, pulp conditioning or water extraction, the same liquid mixture or extracted fluid is gained; an oil-producing microorganism live bacteria solution is inoculated to a sterilizated solid-liquid mixture or the extracted fluid, is cultivated by ventilation shake, and is fermented until the concentration of total reducing sugar in fermenting mash decreases below 1 per cent; the oil-containing thallus is collected, and the microbial oil is extracted by a regular organic solvent extraction method. The invention can decreases the microbial oil raw material cost, features the simple technique and high oil yield and 5.0 to 10.0g dry fungus and 2.5 to 5.0g microbial oil can be gained from per 100g fresh jerusalem artichoke tubers (with 70 per cent to 80 per cent water content).

Description

technical field [0001] The invention relates to a method for preparing microbial oil, in particular to a method for preparing microbial oil by using Jerusalem artichoke tubers as raw materials. Background technique [0002] Microbial oil, also known as single-cell oil (SCO), is made by certain microorganisms, such as yeast, mold, algae, etc., under certain conditions, convert excess carbohydrates into fatty acid glycerides and store them in the bacterial cells. Some bacteria can accumulate more than 50% of their cell dry weight in the medium with glucose as the carbon source. The fatty acid composition of most microbial oils is similar to that of common vegetable oils such as rapeseed oil, palm oil, and soybean oil, mainly containing palmitic acid, palmitoleic acid, stearic acid, oleic acid, and polyunsaturated fatty acids. The use of microorganisms to produce oil has the advantages of not being affected by seasons and climates, wide sources of raw materials, short producti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/00C12P7/64
CPCC12P7/649C11C3/003Y02E50/13C11B1/10C10L1/026C11C3/12Y02T50/678C10L1/08C11B1/025C12P7/6463C10G2300/1011Y02E50/10Y02P30/20
Inventor 华艳艳赵宗保刘波
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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