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Method for producing oil by mixed culture of yeast and alga

A technology of mixed culture and yeast, which is applied in the field of microbial fermentation, can solve few problems, and achieve the effects of high oil yield, simple operation and high practical value

Inactive Publication Date: 2011-06-01
BEIJING UNIV OF CHEM TECH
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  • Abstract
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  • Claims
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Problems solved by technology

[0006] However, at present, there are few studies on the production of oil by the mixed culture of two microorganisms, especially whether the mixed culture of heterotrophic yeast and autotrophic algae is more conducive to microbial growth and oil synthesis. There are few relevant research reports, and the use of industrial wastewater ( Starch, monosodium glutamate, citric acid, papermaking and other industrial wastewater) mixed culture microorganisms to produce oil reports are also very few

Method used

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  • Method for producing oil by mixed culture of yeast and alga
  • Method for producing oil by mixed culture of yeast and alga

Examples

Experimental program
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Effect test

Embodiment 1

[0034] The comparison of embodiment 1 Rhodotorula viscosus and Spirulina platensis fermented in mixed culture medium and separate culture to produce oil

[0035] The microorganisms used were Rhodotorula viscosus CGMCC No.2258 (purchased from the China General Microorganism Culture Collection Center) and Spirulina platens FACHB-Collection No.791 (purchased from the Type Culture Collection Committee of the Chinese Academy of Sciences). species library). The formula of seed liquid culture medium is:

[0036] Rhodotorula viscosus: glucose 4g; yeast powder 0.15g; (NH 4 ) 2 SO 4 0.2g; KH 2 PO 4 0.7g; Na 2 SO 4 0.2g; MgSO 4 ·7H 2 O 0.15g, dilute to 100ml with water, adjust pH to 5.5.

[0037] Spirulina platensis: NaHCO 3 16.8g; K 2 HPO 4 0.5g; NaNO 3 2.5g; NaCl 1.0g; MgSO 4 ·7H 2 O 0.2g; K 2 SO 4 1.0g; FeSO 4 ·7H 2 O 10mg; EDTA 80mg; CaCl 2 4 mg; H 3 BO 3 2.86mg; (NH 4 ) 6 Mo 7 o 24 0.02mg; MnCl 2 4H 2 O 1.8 mg; CuSO 4 ·5H 2 O 0.125 mg; ZnSO 4 ·7H 2 O...

Embodiment 2

[0051] Example 2 Mixed cultivation of Rhodosporidium toruloides and Spirulina platensis in a stirred tank to produce microbial oil by batch fermentation

[0052] The microorganism used is Rhodosporidium toruloides (Rhodosporidium toruloides) CGMCC No.2.1609, purchased from China General Microorganism Culture Collection and Management Center, Spirulina platensis (same example 1). The formula of seed liquid culture medium is:

[0053] Rhodosporidium toruloides: glucose 20g; yeast powder 1.5g; (NH 4 ) 2 SO 4 0.1g; KH2 PO 4 0.4g; MgSO 4 ·7H 2 O1.5g, dilute to 1L with water, adjust pH to 6.0.

[0054] Spirulina platensis: NaHCO 3 16.8g; K 2 HPO 4 0.5g; NaNO 3 2.5g; NaCl 1.0g; MgSO 4 ·7H 2 O 0.2g; K 2 SO 4 1.0g; FeSO 4 ·7H 2 O 10mg; EDTA 80mg; CaCl 2 4 mg; H 3 BO 3 2.86mg; (NH 4 ) 6 Mo 7 o 24 0.02mg; MnCl 2 4H 2 O 1.8 mg; CuSO 4 ·5H 2 O 0.125 mg; ZnSO 4 ·7H 2 O 0.22mg, dilute to 1L with water.

[0055] The mixed medium is an optimized medium, the formul...

Embodiment 3

[0063] Example 3 Mixed cultivation of rhodotorula viscosus and chlorella fed-batch fermentation to produce microbial oil in a stirred tank

[0064] The microorganisms used were Rhodotorula viscosus (same example 1) and Chlorella vulgaris FACHB-Collection No.791 (Chlorella vulgaris was purchased from the Freshwater Algae Species Bank of the Type Culture Collection Committee of the Chinese Academy of Sciences).

[0065] The formula of seed liquid culture medium is:

[0066] Rhodotorula viscosus: glucose 4g; yeast powder 0.15g; (NH 4 ) 2 SO 4 0.2g; KH 2 PO 4 0.7g; Na 2 SO 4 0.2g; MgSO 4 ·7H 2 O 0.15g, dilute to 100ml with water, adjust pH to 5.5.

[0067] Chlorella: NaNO 3 1.5g; K 2 HPO 4 ·3H 2 O 0.04g; MgSO 4 ·7H 2 O 0.075g; CaCl 2 2H 2 O 0.036g; Citric acid 0.006g; Ferric ammonium citrate 0.006g; EDTA 0.001g; 2 CO 3 0.02g; A 5 1mL; add water to make up to 1L. A 5 Recipe: H 3 BO 3 2.86g; MnCl 2 ·H 2 O 1.81g; ZnSO 4 ·7H 2 O 0.222g; CuSO 4 ·5H 2 O 0.0...

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Abstract

The invention discloses a method for producing oil by mixed culture of yeast and alga. In the method, industrial waste water is used as a culture medium for the mixed culture of yeast and alga, so as to produce microbial oil. The method is characterized in that compared with the single culture of two microorganisms, by the mixed culture of yeast and alga, the chemical oxygen demand (COD) of the waste water can be more effectively degraded and the yeast and alga are also promoted to reliably utilize the nutrient components in the industrial waste water, thereby being beneficial to the synthesis of the oil. The method for producing the microbial oil has the advantages of low cost and high practicable value, is simple to operate, namely, the pollution of high-concentration industrial waste water on environment can be reduced, and waste materials are changed into valuable things so as to produce shortage product oil, thereby achieving the purpose of recycling the waste materials.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, in particular to a method for cultivating oil microorganisms to ferment and produce oil. Background technique [0002] With the increasing pressure on human social resources and environmental problems, especially with the increasingly serious global energy shortage and environmental deterioration, people are actively developing renewable and clean new energy to replace fossil fuels. Biodiesel is a kind of energy with great development. Potential renewable clean energy. The main sources of biodiesel oil, animal oil and vegetable oil, can no longer meet the production needs. Therefore, the production of oil through microbial transformation of renewable resources and the development and research of microbial oil, a new oil resource, not only enrich the traditional oil industry technology. , and will also be an important way for industrialized production of oil. [0003] Oleaginous microorgani...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P7/64C12R1/72C12R1/865C12R1/645C12R1/89
Inventor 张栩赵桂丽谭天伟苗金鑫
Owner BEIJING UNIV OF CHEM TECH
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