42 results about "Autotrophic organism" patented technology

The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently convert carbon dioxide and light into n-alkanes, and in particular the use of such organisms for the commercial production of n-alkanes and related molecules.

The invention discloses a device and a method for denitrification of single stage autotroph in low-cellulose nitrate (CN) high-ammonia nitrogen waste water. The device comprises a raw water tank, a water inlet pump, a reactor, a secondary sedimentation tank and a sludge reflux pump, wherein overcurrent holes are arranged in the water flow direction of the reactor in an up-and-down alternative form and connected with various grid chambers, an anoxic zone grid chamber is arranged at the front end of the reactor, and an aerobic zone grid chamber is arranged at the back end of the reactor; the anoxic zone grid chamber is provided with a stirrer and an agitator blade; the aerobic zone grid chamber is provided with the stirrer, the agitator blade, an aeration riser pipe and an intermediate perforated clapboard; and the aeration riser pipe is internally provided with an aeration head, and sponge filling material is filled below the intermediate perforated clapboard, wherein filling ratio is 30-50%. In the method, shortcut nitrification is achieved above the aerobic zone through low dissolved oxygen (DO is 0.5 <-1>mg / L) and free ammonia (FA) inhibition so that ammonia nitrogen is converted to nitrite nitrogen; and anoxicammoxidation biomembrane acts on the lower part of the aerobic zone, the nitrite nitrogen and ammonia nitrogen are converted to nitrogen, thereby achieving denitrification of autotroph. The method has the advantages of low oxygen consumption, less sludge output and no extra carbon source.

An economical method for recovering nitrogen from liquid waste using autotrophic organisms and minimal energy inputs and without chemical additives. Solids are separated from anaerobically digested liquid waste. The resulting translucent liquid is introduced to a culture of autotrophic microorganisms in the presence of natural or artificial light, thereby accumulating biomass and producing a liquid effluent with elevated pH. The elevated-pH, liquid effluent is heated and stripped of ammonia, thereby producing a water vapor and stripped ammonia gas stream. The water vapor ammonia gas stream is condensed to form a liquid / ammonia condensate. The autotrophic microorganisms are advantageously cultivated in a photobioreactor comprising a plurality of axially spaced-apart, growth plates mounted for rotation to a shaft. The pH of the culture is adjustable within a preferred range of 8.0 to 10.5 by adjusting the light intensity and rotational speed.

An efficient system and process for removing nitrogen from wastewater while enriching seed sludge in the mainstream treatment process. Bioaugmentation of seed autotrophic organisms facilitate the nitrification reactions by enhancing the rates of reaction advantageously within a smaller volume or within a shorter activated sludge solids retention time. Likewise, bioaugmentation of seed denitrification organisms will also enhance rate of reaction within a smaller volume or shorter activated sludge solids retention time. Separate treatment of high ammonia digester reject water is an efficient method to treat nitrogen in recycle streams as well as to enrich the seed nitrifying and denitrifying cultures.

The invention provides a preparation method of transgenic dunaliella salina for improving the photosynthesis efficiency, which is characterized by comprising the following steps: colonizing or reforming genes related to carbon fixation and photosynthesis in a photosynthetic autotroph; transferring the genes into a chloroplast genome or nuclear genome of the dunaliella salina for expressing; successively and selectively culturing; and finally obtaining a stable transgenic dunaliella salina strain. By introducing independent or combined expression of high-efficiency genes related to carbon fixation and photosynthesis, the invention improves the concentration and the transferring speed of CO2 in the transgenic dunaliella salina and ensures that the capability of the transgenic dunaliella salina using the CO2 for photosynthesis can be improved, thereby promoting the autotrophic growth of the transgenic dunaliella salina, accelerating and increasing the accumulation of biomass liveweight of dunaliella salina cells and meeting the requirement on scale production of biodiesel and metabolin thereof as well as genetic engineering products.

The invention relates to the technical field of wastewater treatment, in particular to a sewage treatment device and method for enhancing autotrophic nitrogen removal and synchronous phosphorus recovery of municipal sewage. The sewage treatment device provided by the invention comprises a chemical phosphorus removal area and an autotrophic biological nitrogen removal area which communicate in sequence, wherein the chemical phosphorus removal area comprises a mixing area, a flocculation precipitation area and an anaerobic fermentation area; the autotrophic biological nitrogen removal area comprises an inner aerobic area and a biological membrane carrier distributed on the outer side of the inner aerobic area; a dosing device is arranged at the bottom of the autotrophic biological nitrogen removal area. The device further comprises a backflow system, wherein a water inlet of the baclflow system communicates with the autotrophic biological nitrogen removal area, and a water outlet of thebaclflow system communicates with the mixing area of the chemical phosphorus removal area. When the device provided by the invention is used for removing nitrogen and phosphorus from municipal sewage,the material consumption and energy consumption can be reduced, and the nitrogen and phosphorus removal efficiency can be improved.

The invention discloses a process for autotrophic biological denitrification of urban secondary effluent. The process specifically comprises the following steps: dissolving pyrite in a pyrite dissolving tank, feeding the dissolved pyrite and urban secondary effluent to be treated into a biological membrane denitrification tank, and carrying out autotrophic biological denitrification and synergistic phosphorus removal. Pyrite and biological nitrogen removal are separated, an acid is added into the pyrite dissolving tank to promote dissolution of pyrite and improve the solubility of pyrite, ferrous ions, elemental sulfur and sulfur ions which can serve as electron donors are generated at the same time, rapid growth of sulfur autotrophic microorganisms is achieved, and the nitrogen and phosphorus removal efficiency is improved; and the dosage of the pyrite is adjusted through a flow adjuster and is added according to the nutrient proportion requirement of the autotrophic denitrification microorganisms, and waste of the pyrite is reduced. The process overcomes the defects of low solubility of pyrite, low denitrification rate and difficulty in controlling the proportion of the nutrientmedium in the denitrification process in the traditional autotrophic denitrification system, enhances the nitrogen and phosphorus removal speed, and shortens the hydraulic retention time of a reactionzone.

The present disclosure relates to methods and compositions for engineering photo autotrophic organisms to convert carbon dioxide and light into fatty acid esters and other molecules, including biofuels. The molecules are then secreted by the organism into a growth medium.

The invention relates to an apparatus for treating coking wastewater through immobilized MBR autotroph nitrogen removal, and a method thereof. The apparatus is composed of a water feeding system, an aeration system, a membrane bioreactor, a water discharging system and a water-bath circulating system. An autotroph nitrogen removal technology is carried out by adopting the membrane bioreactor, a microbe immobilization technology is introduced, and a carrier used by microbe immobilization is arranged in the membrane bioreactor to improve the running performances of the reactor, so microenvironment suitable for growth of aerobic ammonia oxidizing bacteria and anaerobic ammonium oxidation bacteria is provided, and the membrane pollution is alleviated. The method has the advantages of no external carbon source, good wastewater treatment effect and low cost.

The invention discloses a chlorella-containing 3D printed wire and a preparation method thereof. The 3D printed wire is composed of 59-91 parts by weight of a base resin, 8-40 parts by weight of chlorella and 0.05-2 parts by weight of white oil. The preparation method comprises: (1) drying, (2) weighing, (3) high speed kneading, (4) melting granulation, (5) melt extrusion, (6) cooling traction, (7) coiling, and (8) printing of a specific structure based on a FDM technology. The preparation method utilizes a small-sized single-cell autotrophic organism which is rich in functional groups, can becombined with dye molecules or heavy metal ions and has a low price. Through an efficient and accurate FDM technology, a porous support skeleton of fine particles is prepared. The preparation methodis simple and realizes low energy consumption. The chlorella-containing 3D printed wire as an adsorbent solves the problem that after the polysaccharide in forms of a solution, powder, microsphere andgel treats the dye, the adsorbent is difficult to separate from the solution so that secondary pollution is produced. The chlorella-containing 3D printed wire realizes green circulation and high efficiency use.

The invention discloses a device with complex function to intensify the desulfuration of autotrophic organisms, belonging to the water treatment technical field. The device mainly comprises a cathode chamber and an anode chamber which are separated by a proton membrane; wherein, the anode chamber is internally put with dimensional stability anode; the cathode chamber mainly comprises an uniform water-locator and a multifunction three-dimensional compound biology cathode which mainly comprises a hydrogen supply system air inlet chamber, membrane wires, an electric conduction porous flexible carbon material clad on the surface of the membrane wires and a diversion rod which is fixed on one side of the flexible carbon material and is convenient for external circuit to be connected; sulfate reducing bacteria is attached to the electric conduction porous flexible carbon material carrier and the surface of the membrane wires; the external circuit is supplied by power supply. The device has the advantages of high treatment efficiency, low consumption, stable operation and low investment.

The invention relates to a distributed domestic sewage integrated treatment system and process. The distributed domestic sewage integrated treatment system comprises pretreatment equipment, regulationequipment, integrated equipment and disinfection equipment which are connected in sequence, wherein the integrated equipment comprises an anaerobic tank, a primary aerobic tank, a primary anoxic tank, an autotrophic denitrification nitrogen removal tank, an adsorption phosphorus removal tank and a filter tank which are connected in sequence. The process has the beneficial effect of deep nitrogenand phosphorus removal. Compared with other similar integrated processes, the process has the advantages that an integrated autotrophic biological nitrogen and phosphorus removal process is adopted, the treatment efficiency on total nitrogen is higher, and the total nitrogen in effluent can be stably reduced to 5-8 mg / L and far exceeds the current emission standard, the anoxic denitrification process section provided by the invention is placed after aerobic nitrification, is different from a treatment mode of firstly denitrifying and then nitrifying and then refluxing nitrified liquid in a traditional denitrification process, and has the advantages of high denitrification efficiency, low denitrification energy consumption and simple process flow.

An apparatus for treating municipal sewage by using a partial nitrosation-anaerobic ammonia oxidation process is composed of an anaerobic ammonia-oxidizing bacterium enriching and culturing device anda continuous flow PN / A autotrophic biological denitrification device. The anaerobic ammonia-oxidizing bacterium enriching and culturing device comprises a water distribution tank, a liquid medicine barrel, an enriching and culturing reactor and a control system, the continuous flow PN / A autotrophic biological denitrification device comprises a water inlet pump, a PN / A reactor, a sedimentation tank, a sludge reflux pump and a sludge discharge pump, the water inlet pump pumps municipal sewage subjected to carbon removal pretreatment, an aeration device is arranged at the bottom of the PN / A reactor, the air pipe of the aeration device is connected with an air blower, the inlet of the PN / A reactor is connected with the water inlet pump, the outlet of the PN / A reactor is connected with the inlet of the sedimentation tank, a sludge discharge pipe at the bottom of the sedimentation tank is respectively connected with the sludge discharge pump and the sludge reflux pump, and a pipeline for connecting the sludge discharge pipe with the sludge reflux pump is connected to a pipeline for connecting the water inlet pump with the PN / A reactor. The apparatus has the advantages of high starting speed, low effluent nitrate concentration, stability in operation, and suitableness for biological denitrification of municipal sewage.

An efficient system and process for removing nitrogen from wastewater while enriching seed sludge in the mainstream treatment process. Bioaugmentation of seed autotrophic organisms facilitate the nitrification reactions by enhancing the rates of reaction advantageously within a smaller volume or within a shorter activated sludge solids retention time. Likewise, bioaugmentation of seed denitrification organisms will also enhance rate of reaction within a smaller volume or shorter activated sludge solids retention time. Separate treatment of high ammonia digester reject water is an efficient method to treat nitrogen in recycle streams as well as to enrich the seed nitrifying and denitrifying cultures.

The invention discloses a method for starting a one-stage SBR-anammox system. According to the method, a one-stage partial nitrosation anaerobic ammonia oxidation system is started in a manner of starting short-cut nitrosation and then starting anaerobic ammonia oxidation. By controlling the ammonia nitrogen concentration of inlet water, HRT and DO are respectively used for starting short-cut nitrification and anaerobic ammonia oxidation for 75d and 58d. The method comprises the following steps: (1) adding a certain proportion of filler into a reactor in a short-cut nitrification stage; (2) taking return sludge of a secondary sedimentation tank as inoculation sludge, taking simulated sewage as test water, strictly controlling DO (dissolved oxygen), temperature, pH (potential of hydrogen) and inflow matrix concentration, and directly starting short-cut nitrification after aeration succeeds; and (3) inoculating a small amount of anaerobic ammonia oxidation sludge into the reactor to quickly start anaerobic ammonia oxidation so as to start one-stage partial nitrosation anaerobic ammonia oxidation. The method has the effects that partial nitrosation anaerobic ammonia oxidation system is started by adding the filler, changing the impact load and balancing the dominant bacteria of the system under the operating conditions, so that full-autotrophic biological denitrification is realized, and the operating cost is reduced.

The invention provides a device for growing genetically enhanced aquatic photoautotrophic organisms in a stable culture, causing said organisms to produce ethanol, and then separating, collecting, and removing the ethanol in situ.

The municipal sewage denitrification treatment device comprises a reaction tank, the reaction tank is connected with a water inlet pipeline, a water outlet pipeline and a backflow pipeline, and the backflow pipeline is communicated with the water inlet pipeline through a backflow element; a surface aerator is arranged in the reaction tank; the reaction tank comprises a reaction area and a settling area, the settling area is located at the bottom of the reaction area, the reaction area is provided with a deflector, the deflector is provided with a plurality of baffling channels, the side walls of the baffling channels are rough surfaces, and microorganisms in the reaction tank can be attached to the side walls of the baffling channels. The invention further provides a treatment method adopting the municipal sewage denitrification treatment device, nitrosation is started by means of original microorganisms in the wastewater, the microorganisms are attached to the baffling channels to grow, anaerobic ammonia oxidation is started by inoculating anaerobic ammonia oxidation sludge, autotrophic biological denitrification of the municipal sewage is achieved, and material consumption of denitrification treatment is reduced.

The present disclosure relates to an integrated wastewater treatment apparatus and method, the apparatus comprises a first reactor module, a second reactor module, a sedimentation module and a gas-liquid separation module, the first reactor module comprises a first reactor and an anoxic reaction zone, an aerobic reaction zone, a first gas-gathering pressurized layer, a first water inlet pipe and an aeration device; the second reactor module comprises a second reactor, a second water inlet pipe, an anaerobic reaction zone and a second gas-gathering pressurized layer; the sedimentation module comprises a third reactor and a water outlet pipe; the gas-liquid separation module comprises a gas-liquid separator, an exhaust pipe, a first riser pipe, a second riser pipe and a return pipe. The apparatus can give full play to the advantages of the autotrophic biological denitrification process, meet the biochemical treatment requirements of wastewater with low C / N ratio.

The invention relates to a method for remediating lead-polluted soil by a microalgae-montmorillonite compound, which comprises the steps of S1, putting powdery montmorillonite into a microalgae solution which is cultured in advance, uniformly mixing and reacting to obtain a microalgae-montmorillonite compound mixed solution, and controlling the dry weight ratio of microalgae to montmorillonite to be 1: (1-5); and S2, adding the obtained microalgae-montmorillonite compound mixed solution into the lead-polluted soil, and carrying out remediation treatment for a period of time. The method can effectively reduce the free state and reducible state of lead in the high-concentration lead-polluted soil, and has excellent lead-polluted soil passivation performance. The microalgae-montmorillonite compound is environment-friendly, does not cause secondary pollution and can increase soil nutrients, and the microalgae belongs to autotrophic organisms and has relatively low nutritional requirements, so that the method has remarkable economic and ecological effects.

The invention relates to an integrated sewage treatment device which comprises a first reactor module, a second reactor module, a settling module and a gas-liquid separation module; the first reactormodule comprises a first reactor, an anoxic reaction zone, an aerobic reaction zone, a first gas collecting and pressurizing layer, a first water inlet pipe and an aeration device; the second reactormodule comprises a second reactor, a second water inlet pipe, an anaerobic reaction zone and a second gas collecting and pressurizing layer; the settling module comprises a third reactor and a water outlet pipe; the gas-liquid separation module comprises a gas-liquid separator, an exhaust pipe, a first riser pipe, a second riser pipe and a backflow pipe. The device provided by the invention can give full play to the advantages of an autotrophic biological denitrification process, meets the biochemical treatment requirements of sewage with a low C / N ratio, and has the advantages of high denitrification efficiency, low treatment energy consumption, avoidance of addition of chemicals, high space utilization rate, simplicity in maintenance and the like.

The invention discloses a device and a method for denitrification of single stage autotroph in low-cellulose nitrate (CN) high-ammonia nitrogen waste water. The device comprises a raw water tank, a water inlet pump, a reactor, a secondary sedimentation tank and a sludge reflux pump, wherein overcurrent holes are arranged in the water flow direction of the reactor in an up-and-down alternative form and connected with various grid chambers, an anoxic zone grid chamber is arranged at the front end of the reactor, and an aerobic zone grid chamber is arranged at the back end of the reactor; the anoxic zone grid chamber is provided with a stirrer and an agitator blade; the aerobic zone grid chamber is provided with the stirrer, the agitator blade, an aeration riser pipe and an intermediate perforated clapboard; and the aeration riser pipe is internally provided with an aeration head, and sponge filling material is filled below the intermediate perforated clapboard, wherein filling ratio is 30-50%. In the method, shortcut nitrification is achieved above the aerobic zone through low dissolved oxygen (DO is 0.5 <-1>mg / L) and free ammonia (FA) inhibition so that ammonia nitrogen is converted to nitrite nitrogen; and anoxicammoxidation biomembrane acts on the lower part of the aerobic zone, the nitrite nitrogen and ammonia nitrogen are converted to nitrogen, thereby achieving denitrification of autotroph. The method has the advantages of low oxygen consumption, less sludge output and no extra carbon source.

The present invention relates to autotrophic microorganisms with altered photorespiration and improved CO2 fixation as well as a method of producing said autotrophic microorganisms. Particularly, the autotrophic microorganisms show an improved growth rate, productivity and energy conversion efficiency.

An integrated autotrophic biological nitrogen removal collaborative chemical phosphorus removal reactor, which comprises a reactor body, a plurality of partitions and sleeves. The reactor body is provided with a water distribution unit, a deslagging unit, a reaction unit, a three-phase separation unit and a water outlet exhaust unit from the bottom to top. The reaction unit is divided by the partitions and the sleeves into a whole-process autotrophic denitrification reaction zone, a chemical phosphorus removal zone, a reflux dissolution zone and a denitrification nitrogen removal zone. The water distribution unit is provided with a water inlet and an aeration head. The deslagging unit is located at the bottom end of the reflux dissolution zone and chemical phosphorus removal zone of the reactor, and is provided with a slag discharging port. The three-phase separation unit is arranged on the top of the whole-process autotrophic nitrogen removal reaction zone and the denitrification zone and is provided with a flow guide partition. The water outlet exhaust unit comprises a water outlet tank, a water outlet, a gas collecting hood and an exhaust port. The invention has the beneficial effects that dissolved iron, slag and other industrial wastes are used to provide a nitrogen matrix, so as to save carbon sources, reach thorough denitrification, realize treatment of wastes with processes of wastes against one another, recycling of the phosphorus resource waste and achieve many things at one stroke.

The invention discloses a denitrification device and method for coupling a microbial fuel cell (MFC) with a whole-course autotrophic dynamic membrane reactor, and belongs to the technical field of biological sewage treatment. The device comprises an MFC assembly, a whole-course autotrophic bio-membrane reactor, an aeration control system and a dynamic membrane separation assembly, the MFC assembly is used for inlet water carbon removal pretreatment and power generation, the whole-course autotrophic bio-membrane reactor realizes total nitrogen efficient treatment, and the dynamic membrane separation assembly realizes mud-water separation. The method comprises the following steps: controlling the flow direction of wastewater, controlling system parameters and controlling water quality parameters, and finally realizing the synergistic effect of electrogenesis bacteria, ammonia oxidizing bacteria and anaerobic ammonia oxidizing bacteria to realize efficient water treatment. The device integrates the advantages of the MFC, the whole-course autotrophy and the membrane bioreactor, has the advantages of saving the aeration rate, being low in sludge yield, high in load resistance and the like, realizes electrochemical recovery of a carbon source, and is particularly suitable for treating high-ammonia-nitrogen industrial wastewater with a low carbon-nitrogen ratio.

According to the method, hydroxide denitrifying bacteria and anaerobic ammonium oxidation bacteria are adopted for combined denitrification, ammonium radicals and nitrate radicals in wastewater are converted into nitrogen, nitrate nitrogen and ammonia nitrogen in the wastewater are deeply removed at the same time, an organic carbon source does not need to be additionally added, and complete autotrophy is achieved; the system can be operated for a long time, the total nitrogen in the effluent of the bioreactor is less than or equal to 1mgN / L, and the water quality can reach the surface water class III standard.

The invention discloses a device with complex function to intensify the desulfuration of autotrophic organisms, belonging to the water treatment technical field. The device mainly comprises a cathode chamber and an anode chamber which are separated by a proton membrane; wherein, the anode chamber is internally put with dimensional stability anode; the cathode chamber mainly comprises an uniform water-locator and a multifunction three-dimensional compound biology cathode which mainly comprises a hydrogen supply system air inlet chamber, membrane wires, an electric conduction porous flexible carbon material clad on the surface of the membrane wires and a diversion rod which is fixed on one side of the flexible carbon material and is convenient for external circuit to be connected; sulfate reducing bacteria is attached to the electric conduction porous flexible carbon material carrier and the surface of the membrane wires; the external circuit is supplied by power supply. The device has the advantages of high treatment efficiency, low consumption, stable operation and low investment.