Fully autotrophic biological denitrification device and method

An autotrophic biological denitrification and bioreactor technology, applied in the field of biological denitrification, can solve problems such as difficult control, failure to meet denitrification requirements, and inability to remove nitrate, achieve high denitrification rate and reduce energy consumption and cost effects

Active Publication Date: 2022-04-19
XIAMEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the actual operation process, it is necessary to control dissolved oxygen, hydraulic retention time and other measures to make the nitrification process only reach the nitrite step and prevent further oxidation of nitrite to nitrate. Although this type of denitrification technology does not require the addition of organic matter , but it is difficult to control
In addition, in addition to ammonia nitrogen, the actual wastewater usually contains a lot of nitrate nitrogen. The traditional anammox denitrification method cannot remove nitrate nitrogen in wastewater, and cannot meet the denitrification requirements.

Method used

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  • Fully autotrophic biological denitrification device and method
  • Fully autotrophic biological denitrification device and method
  • Fully autotrophic biological denitrification device and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Such as figure 1 As shown, a fully autotrophic biological denitrification device includes a bioreactor 1 and a gas generator 2, the bioreactor 1 includes a water inlet 11 and a water outlet 12, and the interior of the bioreactor 1 is filled with A porous carrier 3, the porous carrier 3 includes a first reaction section 31 and a second reaction section 32 respectively adsorbed with corresponding denitrification microorganisms, the gas generator 2 is used to generate a mixed gas and introduce the mixed gas into the Inside bioreactor 1.

[0040] A gas disperser 4 is placed near the water inlet 11 in the bioreactor 1, and the gas disperser 4 is used to disperse the mixed gas. The side wall of the bioreactor 1 is respectively provided with a first sampling port 13 and a second sampling port 14 at the first reaction section 31 and the second reaction section 32, and the first sampling port 13 and the second sampling port 14 is used for real-time sampling and observation of ...

Embodiment 2

[0045] 1. Screening of hydrogen oxidizing denitrifying bacteria

[0046] The source of the strains to be screened is the sludge in the denitrification tank of a sewage treatment plant, and an appropriate amount of strains to be screened is added to the prepared autotrophic denitrification medium. The composition of the autotrophic denitrification medium is: KNO 3 1g / L, NaHCO 3 1.2g / L,KH 2 PO 40.2g / L. Use 150mL anaerobic bottle for airtight culture, the liquid volume in the anaerobic bottle is 50mL, and the bottle is filled with excess hydrogen. Sampling and measuring the change of its nitrate concentration every day, until the nitrate concentration is reduced to below 0.5mg N / L, add nitrate and hydrogen, after adding two nitrates, transfer the bacterial solution, the transfer amount is 20% ( 10 mL), the composition of the medium was the same as above. After all the nitrates were reduced to nitrogen, transfer again, repeat this three times, and then use the method of anae...

Embodiment 3

[0050] Biocarrier immobilization of hydrogen oxidizing denitrifiers and anammox bacteria:

[0051] The bioreactor of this embodiment has a diameter of 10 cm and a height of 100 cm, and is filled with ceramic porous materials with a diameter of about 5 mm, and the voids of the ceramic materials are 60%.

[0052] The simulated wastewater contains ammonia nitrogen, nitrite nitrogen and nitrate nitrogen, and the specific components are as follows: NH 4 Cl 0.31g / L, NaNO 2 0.35g / L, KNO 3 0.5g / L, NaHCO 3 1.2g / L,KH 2 PO 4 0.2g / L.

[0053] A gas disperser is installed at the bottom of the bioreactor, and the mixed gas of hydrogen and carbon dioxide produced by methanol cracking is fed into it. The gas produced by cracking methanol includes 70% hydrogen and about 25% carbon dioxide.

[0054] An appropriate amount of ceramic porous material is put into the pure cultured Thauera mechernichensis denitrifying bacteria and the bacteria solution of the enriched and purified anammox bac...

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Abstract

According to the method, hydroxide denitrifying bacteria and anaerobic ammonium oxidation bacteria are adopted for combined denitrification, ammonium radicals and nitrate radicals in wastewater are converted into nitrogen, nitrate nitrogen and ammonia nitrogen in the wastewater are deeply removed at the same time, an organic carbon source does not need to be additionally added, and complete autotrophy is achieved; the system can be operated for a long time, the total nitrogen in the effluent of the bioreactor is less than or equal to 1mgN/L, and the water quality can reach the surface water class III standard.

Description

technical field [0001] The invention relates to the technical field of biological denitrification, in particular to a fully autotrophic biological denitrification device and method. Background technique [0002] Existing biological denitrification of wastewater mainly adopts nitrification-denitrification technology. First, ammonium is oxidized to nitrite by aerobic ammonia oxidizing bacteria, and then nitrite is oxidized to nitrate by aerobic nitrite oxidizing bacteria. That is, the process of nitrosation and nitrification, and then through the way of adding organic carbon sources, acetic acid, methanol, etc. are introduced, and the nitrate is reduced to nitrogen by denitrifying bacteria, that is, the process of denitrification. The nitrification process is an aerobic autotrophic process, and the denitrification process is an anaerobic heterotrophic process. This type of nitrification-denitrification denitrification technology generally has long process routes, high energy c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/28C02F3/34C02F101/16
CPCC02F3/286C02F3/34C02F2101/16C02F2305/06
Inventor 徐方成李欣叶玲
Owner XIAMEN UNIV
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