Process for efficiently purifying shikimic acid by utilizing continuous ion exchange technology

Abstract

The invention discloses a process for efficiently purifying shikimic acid by utilizing a continuous ion exchange technology, and the process comprises: carrying out solid-liquid separation to obtain afermentation filtrate, and carrying out pre-purification on the fermentation filtrate by virtue of cation exchange resin to obtain a pre-purified liquid a; and pre-purifying the pre-purified liquid athrough a continuous ion exchange system to obtain a pre-purified liquid b; and finally, carrying out fine purification and spray drying on the pre-purified liquid b to obtain the shikimic acid. Theprocess has the advantages that the process is simple and stable, continuous and automatic production can be realized, the chromatographic purity and the content of the prepared shikimic acid finishedproduct are both higher than 99%, the utilization rate of the resin is greatly improved, the total usage amount of the resin is reduced by about 80%, an eluent is not used in the pre-purification process, wastewater is greatly reduced, the production cost is greatly reduced, the market competitiveness of the product is enhanced, and the process is suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the technical field of separation and purification of fermented products, and in particular relates to a process for efficiently purifying shikimic acid by using continuous ion exchange technology. Background technique [0002] Shikimic acid (Shikimic acid, formula I) was first extracted by Eykman in 1885 from the dried ripe fruit of the star anise plant, and its chemical name is 3,4,5-trihydroxy-1-cyclohexene-1-carboxylic acid , the molecular formula is C 7 h 10 o 5 , with a molecular weight of 174.15. [0003] [0004] Shikimic acid can inhibit platelet aggregation, arterial and venous thrombosis and cerebral thrombosis by affecting the metabolism of arachidonic acid. It also has anti-inflammatory and analgesic functions. A key intermediate in the synthesis of Tamiflu, a drug for the prevention and treatment of bird flu. In the past ten years, the avian influenza epidemic has continued to spread around the world, and ...

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Problems solved by technology

[0009] 3) This patent has no other pre-purification steps after being treated by ceramic membrane and ultrafiltration membrane, and directly uses anion exchange resin for adsorption and elution purification, which has low processing capacity of anion exchange resin, low chromatographic purity of the obtained eluent, batch Unsuitable for industrialized production due to large fluctuations in operations between operations

[0010] 4) 3-dehydroshikimic acid is an inherent impurity in the biosynthetic pathway of shikimic acid, which is similar in structure to shikimic acid. Lower resolution from shikimic acid

This process has the following disadvantages: 1. After the fermentation broth is centrifuged, the supernatant is directly concentrated under reduced pressure, which consumes a lot of energy and costs; 2. After the centrifugation, no other pre-purification steps are taken to directly purify the anion exchange resin, resulting in resin processing capacity. Very low; 3. Using twice crystallization, the total yield of the process is low, and it does not have the feasibility of industrialization

Therefore, most of the exchange groups on the anion exchange resin are bound by impurity anions, which leads to a very low effective adsorption capacity of the anion exchange resin for shikimic acid.

Moreover, these impurity anions with strong binding force also reduce the separation degree of 3-dehydroshikimic acid and shikimic acid on anion exchange resin, resulting in serious crossover of eluent and low elution yield of shikimic acid with high chromatographic purity

Poor quality eluents, causing more stress in subsequent purification steps

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