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Method for preparing shikimic acid extract from chromatographic waste liquor of ginkgo biloba extract

A ginkgo extract, extract technology, applied in the separation/purification of carboxylic acid compounds, chemical instruments and methods, silicon compounds, etc., can solve the problem of low content of shikimic acid extract

Inactive Publication Date: 2019-02-22
YANGTZE NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a method for preparing shikimic acid extract from ginkgo leaf extract chromatographic waste liquid, which solves the problem that the content of shikimic acid extract extracted from ginkgo leaf extract chromatographic waste liquid in the prior art is relatively high. low problem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A method for preparing shikimic acid extract from ginkgo biloba extract chromatography waste liquid, specifically implemented according to the following steps:

[0031] Step 1, take the chromatographic flow-through liquid 10L that produces when Ginkgo biloba extract is prepared by macroporous resin column chromatography with Ginkgo biloba extract, the content of shikimic acid in this waste liquid is 0.86%, waste liquid is concentrated under reduced pressure to The volume is 2L, and the pretreated waste liquid is obtained;

[0032] Step 2, using 1 L of chloroform to extract and pretreat the waste liquid twice, retaining the water phase to obtain water phase 1;

[0033] Then use 1L of benzene to extract the water phase one or two times, and keep the water phase to obtain the water phase two;

[0034] Finally, the aqueous phase II was extracted twice with 1 L of petroleum ether, and the aqueous phase was kept to obtain 1.76 L of aqueous phase III;

[0035] Step 3, boil 1...

Embodiment 2

[0040] A method for preparing shikimic acid extract from ginkgo biloba extract chromatography waste liquid, specifically implemented according to the following steps:

[0041] Step 1, take 2 L of chromatographic washing liquid produced when ginkgo leaf extract is prepared by macroporous resin column chromatography, the content of shikimic acid in the waste liquid is 0.63%, and the waste liquid is concentrated under reduced pressure to volume Be 400ml, obtain pretreatment waste liquid;

[0042] Step 2, use 200ml of chloroform to extract and pretreat the waste liquid 3 times, keep the water phase to obtain water phase 1;

[0043] Then use 200ml of benzene to extract the water phase 1 for 3 times, and keep the water phase to obtain the water phase 2;

[0044] Finally, extract the water phase 2 with 200ml of petroleum ether for 3 times, and continue to keep the water phase to obtain 351ml of the water phase 3;

[0045]Step 3, boil 351ml of the water phase three, and keep boiling...

Embodiment 3

[0050] A method for preparing shikimic acid extract from ginkgo biloba extract chromatography waste liquid, specifically implemented according to the following steps:

[0051] Step 1, take the mixed waste liquid 5L of the chromatographic flow-through liquid and the chromatographic washing liquid produced when the Ginkgo biloba extract is prepared by macroporous resin column chromatography, the content of shikimic acid in this waste liquid is 0.75 %, the waste liquid is concentrated under reduced pressure to a volume of 1L to obtain the pretreated waste liquid;

[0052] Step 2, use 500ml of chloroform to extract and pretreat the waste liquid twice, keep the water phase, and obtain the water phase 1;

[0053] Then use 500ml of benzene to extract the water phase one or two times, and continue to keep the water phase to obtain the water phase two;

[0054] Finally, extract the water phase 2 with 500ml of petroleum ether twice, and continue to keep the water phase to obtain 962ml ...

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Abstract

The invention discloses a method for preparing shikimic acid extract from chromatographic waste liquor of a ginkgo biloba extract. The method specifically comprises the following steps: taking waste liquor produced during preparation of the ginkgo biloba extract by macroporous resin column chromatography, and concentrating the waste liquor to the volume of 1 / 5 of the original volume so as to obtain pretreatment waste liquor; extracting the pretreatment waste liquor with chloroform so as to obtain an aqueous phase I; extracting the aqueous phase I with benzene to obtain an aqueous phase II; extracting the aqueous phase II with petroleum ether to obtain an aqueous phase III; diluting the aqueous phase III with water, adding a modified vermiculite adsorbent, stirring, standing, filtering andcollecting filter residues; adding water equivalent to an amount of 50 times that of the weight of the filter residues into the filter residues, performing ultrasonic treatment at a temperature of 60-80 DEG C, filtering after treatment completion, and collecting the filtrate; and performing vacuum concentration on the filtrate, and re-crystallizing, thereby obtaining the shikimic acid extract. According to the method disclosed by the invention, the shikimic acid in the waste liquor produced during preparation of the ginkgo biloba extract by the macroporous resin column chromatography can be well extracted, and the obtained product is high in quality and high in content and has wide application prospects.

Description

technical field [0001] The invention belongs to the technical field of plant purification, and in particular relates to a method for preparing shikimic acid extract from ginkgo biloba extract chromatography waste liquid. Background technique [0002] The composition of Ginkgo biloba is relatively complex, and the chemical components mainly include flavonoid glycosides, terpene lactones, polyprenyl alcohols, 6-hydroxykynurenic acid, organic acids, ginkgolic acids and alkylphenols, alkyl Phenolic acid (also known as ginkgolic acid) 4ˊ-methoxypyridoxine, etc. For a long time, people's research on ginkgo leaves has mainly focused on the extraction of ginkgo terpene lactones and flavonoid glycosides in ginkgo leaves, while other chemical The components are then discarded with the extraction waste. In recent years, since Roche announced that the special anti-avian flu drug "Tamiflu" is made of shikimic acid extracted from star anise as a synthetic raw material, shikimic acid has ...

Claims

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Application Information

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IPC IPC(8): C07C51/42C07C51/48C07C51/47C07C62/32B01J20/10B01J20/30
CPCB01J20/10C07C51/42C07C51/47C07C51/48C07C2601/16C07C62/32
Inventor 尚淑梅高晓旭张宗学杨银周丽娟田玉红陈世美
Owner YANGTZE NORMAL UNIVERSITY
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