Extraction process for shikimic acid in fermentation liquor

An extraction process and fermentation liquid technology, applied in the field of bioengineering, can solve the problems of not being able to use directly, low content of shikimic acid, etc., and achieve the effects of reducing workload, high exchange capacity, and reducing wastewater discharge

Inactive Publication Date: 2012-07-18
河南孟成生物药业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after the successfully constructed production strain is fermented, the content of shikimic acid in the fermented liquid obtained is low and cannot be used directly. Further subsequent extraction and purification treatment is required to obtain the finished product of shikimic acid with high purity.

Method used

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  • Extraction process for shikimic acid in fermentation liquor
  • Extraction process for shikimic acid in fermentation liquor
  • Extraction process for shikimic acid in fermentation liquor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] An extraction process for shikimic acid in a fermented liquid, specifically:

[0028] (1) 1000ml of fermentation broth (the specific gravity of fermentation broth is 0.98, the mass percentage of solid matter is 16%, of which shikimic acid is 30g) passed through Al 2 o 3 The ceramic membrane with a mass percentage of 99% is used for microfiltration, the pore size of the ceramic membrane is 0.1 μm, and the microfiltration flux is 10L / m 2 h.

[0029] (2) The filtrate obtained after microfiltration is ultrafiltered with a roll-type ultrafiltration membrane. The material of the ultrafiltration membrane is polyvinylidene fluoride. The molecular weight cut-off of the ultrafiltration membrane used is 5000 Daltons, and the ultrafiltration flux is 10L / m 2 h.

[0030] (3) The clear night obtained after ultrafiltration is exchanged and adsorbed with 717 anion exchange resin column, the column flow rate is 1BV / h, and the batch adsorption time is 8h; then it is eluted with 1mol / L ...

Embodiment 2

[0033] The difference from Example 1 is: when the eluate is concentrated under reduced pressure at 60° C. to a shikimic acid concentration of about 450 g / L in a concentration pot, powdered activated carbon is added for decolorization treatment, and the others are the same as in Example 1. Obtain 24.1g white powdery crystals at last, be exactly shikimic acid finished product (purity is 98.68%, yield 79.27%, see image 3 ).

Embodiment 3

[0035] An extraction process for shikimic acid in a fermented liquid, specifically:

[0036] (1) 1000ml of fermentation broth (the specific gravity of fermentation broth is 0.98, the mass percentage of solid matter is 18%, of which shikimic acid is 33g) passed through Al 2 o 3 The ceramic membrane with a mass percentage of 99% is used for microfiltration, the pore size of the ceramic membrane is 0.5μm, and the microfiltration flux is 30L / m 2 h.

[0037] (2) The filtrate obtained after microfiltration is ultrafiltered with a roll-type ultrafiltration membrane. The material of the ultrafiltration membrane is polyvinylidene fluoride. The molecular weight cut-off of the ultrafiltration membrane used is 5000 Daltons, and the ultrafiltration flux is 30L / m2 h.

[0038] (3) The clear night obtained after ultrafiltration is exchanged and adsorbed with 717 anion exchange resin column, the column flow rate is 0.5BV / h, and the batch adsorption time is 10h; then it is eluted with 1.5mol / ...

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Abstract

The invention relates to an extraction process for shikimic acid in fermentation liquor. The extraction process specially comprises the steps of conducting microfiltration on the fermentation liquor through a ceramic film to obtain filtrate, conducting exchange adsorption on the filtrate through anion exchange resin, then eluting the filtrate through acetic acid solution to obtain eluent, concentrating, destaining, crystallizing, crystal washing and drying the eluent to obtain finished shikimic acid. The process is simple and environment-friendly, and the finished shikimic acid extracted by the process is stable in quality, the purity of the finished shikimic acid is more than 98.5%, and the yield of the finished shikimic acid is more than 75%.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to an extraction process of shikimic acid in fermentation broth. Background technique [0002] Shikimic acid is the key raw material for Roche to produce Tamiflu. Shikimic acid currently used by Roche is mostly extracted from star anise. Due to the complicated steps of using petrochemical raw materials to synthesize shikimic acid, there is little practical production and application value except for scientific research work. In addition, shikimic acid can also be extracted and synthesized from another natural product, quinic acid. Quinic acid is derived from the bark of the cinchona tree, where it can be extracted and supplied in large quantities like shikimic acid. The cinchona tree is mainly produced in Zaire, an African country. However, due to the unstable political situation in Zaire, it is difficult to ensure a stable commercial supply of cinchona bark. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C62/32C07C51/42
Inventor 张蕴才武广君孟军虎任存邦王春阳徐灵芝董建辉石玲珑叶晓冲崔琳琅乔娟平李晓霞郭丹丹翟娇琳
Owner 河南孟成生物药业股份有限公司
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