Method for preparing shikimic acid extract from folium ginkgo extract chromatography waste liquid

The technology of extract and ginkgo leaf, applied in the field of preparing shikimic acid extract, can solve the problems of unsuitability for ginkgo leaf extraction workshop, high cost of adsorbent, difficulty in scale, etc., and achieves good application value, low cost and large processing capacity Effect

Inactive Publication Date: 2018-06-22
ZHEJIANG WANBANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the high solubility of shikimic acid in water, solvent extraction often has the problem of low separation efficiency
Silica gel column chromatography has disadvantages such as high cost of adsorbent and difficulty in scale-up
Chen Ying and others reported that anion exchange resin can be used as material to purify shikimic acid in pine needle extract of Pinus massoniana. Although the separation efficiency is very high and the purity of shikimic acid obtained is also high, this process requires dilute lye for elution, and Ginkgo biloba is required. There are new types of solvents in the extract workshop, and the production cycle is longer, which is not suitable for the Ginkgo biloba extract workshop

Method used

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  • Method for preparing shikimic acid extract from folium ginkgo extract chromatography waste liquid
  • Method for preparing shikimic acid extract from folium ginkgo extract chromatography waste liquid
  • Method for preparing shikimic acid extract from folium ginkgo extract chromatography waste liquid

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Pass the collected chromatographic washing liquid through the ADS21 type macroporous resin chromatography column at a flow rate of 0.5BV / h, and the ratio of the height to diameter of the chromatographic column is 9:1. The macroporous resin is rinsed at a flow rate of h, and then 4.0BV of 60% ethanol aqueous solution is used for eluting at a flow rate of 0.5BV / h, the eluate is collected, and the collected solution is concentrated and dried under reduced pressure to obtain final product. After HPLC analysis, the obtained Shikimic acid extract with a mass fraction of shikimic acid of 37.7%. The HPLC chart of the eluent is shown in image 3 shown.

Embodiment 2

[0030] The collected chromatographic washing liquid is directly passed through the ADS21 type macroporous resin chromatographic column at a flow rate of 2.0BV / h, and the height-to-diameter ratio of the chromatographic column is 12:1. The macroporous resin was rinsed at a flow rate of 6.0BV / h, and then eluted with 40% ethanol aqueous solution of 6.0BV at a flow rate of 1.5BV / h, the eluate was collected, and the collected solution was concentrated and dried under reduced pressure to obtain final product. After HPLC analysis, A shikimic acid extract with a mass fraction of shikimic acid of 35.0% was obtained.

Embodiment 3

[0032] Pass the collected chromatographic flow-through directly through the NK109 macroporous resin chromatography column at a flow rate of 1.0BV / h. The column height-to-diameter ratio is 12:1. The macroporous resin is rinsed at a flow rate of BV / h, and then eluted with 6.0 BV of 10% ethanol aqueous solution at a flow rate of 2.0 BV / h, and the eluate is collected, and the collected liquid is concentrated and dried under reduced pressure, and analyzed by HPLC , to obtain a shikimic acid extract with a mass fraction of shikimic acid of 43.2%.

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Abstract

The invention relates to a method for preparing shikimic acid extract from folium ginkgo extract chromatography waste liquid. The method specifically comprises the following steps: 1) the shikimic acid extract is prepared from the waste liquid, generated during preparation of folium ginkgo extract with the chromatography technology, as a raw material, wherein the waste liquid comprises chromatography flow-through liquid or washing liquid in the chromatography process; 2) the folium ginkgo extract chromatography waste liquid is enriched and purified by a chromatography column, a washing liquidis added for impurity removal, shikimic acid is eluted with an eluent, an eluate is collected and concentrated, and the shikimic acid extract with shikimic acid mass fraction of 35% or higher is obtained.

Description

technical field [0001] The invention relates to a method for preparing shikimic acid extract from ginkgo biloba extract chromatography waste liquid. Background technique [0002] Shikimic acid is a small molecule organic acid, which exists widely in nature, has anti-inflammatory and analgesic effects, and can inhibit platelet aggregation, venous thrombosis and cerebral thrombosis by affecting the metabolism of arachidonic acid. In 1997, people synthesized Tamiflu for the first time by utilizing the similarity between shikimic acid and oseltamivir phosphate (trade name: Tamiflu). Since the six-membered alicyclic skeleton of the three chiral centers of shikimic acid is very similar to that of Tamiflu, So it is an excellent raw material for the synthesis of Tamiflu. [0003] Shikimic acid is often extracted from the traditional Chinese medicine star anise. However, due to the fact that star anise has few branches worldwide and the production cycle is long, the extraction proce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C51/42C07C51/47C07C62/32
CPCC07C51/42C07C51/47C07C62/32
Inventor 瞿海斌龚行楚陈安陈莉许海军舒志恒杨韬炜
Owner ZHEJIANG WANBANG PHARMA
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