Tissue cultivating method of spiceberry melaleuca alternifolia

A technology of Melaleuca alternifolia and tissue culture, applied in the biological field, can solve problems such as long breeding cycle, lack of provenance, and difficult cuttings

Inactive Publication Date: 2007-09-12
厦门涌泉科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to many factors such as lack of provenance, difficult rooting of cuttings, long breeding cycle, and genetic va

Method used

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Embodiment Construction

[0011] A specific example of using the present invention to carry out the tissue culture of the spice plant Melaleuca alternifolia.

[0012] A. Explant treatment and induction culture

[0013] Cut the axillary buds from the parent body of Melaleuca alternifolia with excellent properties as explants, first clean and disinfect the explants, the specific steps are soaking in washing powder for 10 minutes, and rinsing under clear water for 30 minutes; use 75% Soak in alcohol for 20 seconds, rinse twice with sterile water, and then use 0.1% HgCl 2 Disinfect for 7 minutes, rinse with sterile water for 5 times, absorb the water and cut into stems of about 1cm, then inoculate them on the induction medium on the ultra-clean workbench for induction and differentiation culture, culture conditions: light intensity: 2500-3000Lux , the temperature is 26±1°C. The ratio of induction medium is: 7 / 10MS-MS, 6-BA0.1-0.3mg / L, NAA0.1-0.3mg / L.

[0014] B. Subculture

[0015] After about 30 days ...

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Abstract

The present invention discloses tissue culture process of alternate leaf cajeputtree as one kind of perfume plant. The tissue culture process includes the following steps: cutting axillary bud as the explant from the mother body, washing and sterilizing; the subsequent inducing culturing in inducing culture medium comprising 7/10MS-MS, 6-BA 0.1-0.3 mg/L and NAA 0.1-0.3 mg/L; secondary culturing in secondary culture medium comprising 7/10 MS-MS, 6-BA 0.4-0.6 mg/L and NAA 0.05-0.15 mg/L; and final rooting culturing in rooting culture medium comprising 2/5MS -3/5MS, and IBA 0.5-0.7 mg/L. The tissue culture process has the culturing conditions including illumination intensity of 2500-3000Lux, and temperature of 26+/-1 deg.c. The tissue culture process is suitable for fast propagation in industrial scale.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically refers to a method for tissue culture of spice plant Melaleuca alternifolia. Background technique [0002] Melaleuca altennifolia (Melaleuca altennifolia) is a plant of the genus Melaleuca Myrtaceae, which is native to Australia and is a small evergreen tree suitable for popularization and planting in South China. The tea tree oil extracted from its fresh branches and leaves has the properties of a disinfectant, which can effectively inhibit many ubiquitous pathogenic cells and molds. However, due to many factors such as lack of provenance, difficult rooting of cuttings, long breeding cycle and genetic variation, it is difficult to meet the growing market demand in a short period of time with conventional propagation methods. [0003] Plant tissue culture (Tissue culture) refers to the use of the totipotency of plant cells to take out organs (parts), tissues and cells from plants, s...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 赖桂星林良科石斗开朱文辉陈文炳王建红郭逸成
Owner 厦门涌泉科技有限公司
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