Tissue cultivating method of spiceberry melaleuca alternifolia

Abstract

The present invention discloses tissue culture process of alternate leaf cajeputtree as one kind of perfume plant. The tissue culture process includes the following steps: cutting axillary bud as the explant from the mother body, washing and sterilizing; the subsequent inducing culturing in inducing culture medium comprising 7 / 10MS-MS, 6-BA 0.1-0.3 mg / L and NAA 0.1-0.3 mg / L; secondary culturing in secondary culture medium comprising 7 / 10 MS-MS, 6-BA 0.4-0.6 mg / L and NAA 0.05-0.15 mg / L; and final rooting culturing in rooting culture medium comprising 2 / 5MS -3 / 5MS, and IBA 0.5-0.7 mg / L. The tissue culture process has the culturing conditions including illumination intensity of 2500-3000Lux, and temperature of 26+ / -1 deg.c. The tissue culture process is suitable for fast propagation in industrial scale.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically refers to a method for tissue culture of spice plant Melaleuca alternifolia. Background technique [0002] Melaleuca altennifolia (Melaleuca altennifolia) is a plant of the genus Melaleuca Myrtaceae, which is native to Australia and is a small evergreen tree suitable for popularization and planting in South China. The tea tree oil extracted from its fresh branches and leaves has the properties of a disinfectant, which can effectively inhibit many ubiquitous pathogenic cells and molds. However, due to many factors such as lack of provenance, difficult rooting of cuttings, long breeding cycle and genetic variation, it is difficult to meet the growing market demand in a short period of time with conventional propagation methods. [0003] Plant tissue culture (Tissue culture) refers to the use of the totipotency of plant cells to take out organs (parts), tissues and cells from plants, s...

AI Technical Summary

Problems solved by technology

However, due to many factors such as lack of provenance, difficult rooting of cuttings, long breeding cycle, and genetic variation, it is difficult to meet the growing market demand in a short period of time with conventional propagation methods.